FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680634

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680634
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG82.6058631921824107Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA82.6058631921824107Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT61.9543973941368076Clontech Universal Primer Mix Long (96% over 26bp)
GCTTGTGAAAATCTAGAAAAATATTATTCAACATCCCCACCTACTGGTAA30.9771986970684038No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.9771986970684038Clontech Universal Primer Mix Long (100% over 23bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG30.9771986970684038Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT30.9771986970684038Clontech SMART CDS Primer II A (100% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC30.9771986970684038Clontech SMART CDS Primer II A (96% over 26bp)
AGTCACACCTTCCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTACCA20.6514657980456027No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGAACGCACCC20.6514657980456027Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG20.6514657980456027Clontech Universal Primer Mix Long (96% over 26bp)
AAAGAAACCCCGGTGCGCAGGCGCACGACGGGGTAATAAATCATCTCGGC20.6514657980456027No Hit
CACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.6514657980456027Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.6514657980456027Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG20.6514657980456027Clontech Universal Primer Mix Long (96% over 26bp)
TCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.6514657980456027Clontech Universal Primer Mix Long (96% over 26bp)
AAGTAAAAACTAATCATAACAAACAAATCAGACAACATCCAAAGAATTTC20.6514657980456027No Hit
GTACATGGGTGATACGGCGCCACCGAGATCTACACTCTTTCCCTACACGA20.6514657980456027Illumina Single End PCR Primer 1 (100% over 31bp)
TCCATGTACTCTGCGTTGATACCACCGCTGTCTCTTATACACATCTGACG20.6514657980456027Clontech SMARTer II A Oligonucleotide (95% over 24bp)
GTACATGGGAATGAACGGCGACCACCGAGATCTACACTCTTCCCTACACG20.6514657980456027Illumina Single End PCR Primer 1 (96% over 28bp)
TGTCTAGATTGCGTAGTGGGCAAGTCAGGGCTTTTAGGGTACCCATTACC20.6514657980456027No Hit
CAATTACCCAGTGAATCTGGGATGAACTCTATGTGGCAAGTGGTGCTGTC20.6514657980456027No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20.6514657980456027Clontech SMART CDS Primer II A (100% over 26bp)
ATTTCCTTATCCTTCCATCCATTTATTCATTCACGGGTCACATCATCTAT20.6514657980456027No Hit
GCTGGAGAGCCGGCCGCTGCCCCCTCGTCGCCGCATCACACTCCCGTCCC20.6514657980456027No Hit
GACCTCTGCTTCCCAGGTTCAAGTAGTTCTCATGCCTCAGCCTCCTGAAT20.6514657980456027No Hit
CAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAGGGA20.6514657980456027Illumina Single End PCR Primer 1 (100% over 25bp)
GTACATGGGCAATGATACGGCGACCACCGAGATCTACACTCATTCCCTAC10.32573289902280134Illumina Single End PCR Primer 1 (97% over 40bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAACAAG10.32573289902280134No Hit
GAGTACATGGGAGGGCACGAGATACCTTTCCCTACACGACGCTCGTTTTT10.32573289902280134No Hit
GTACATGGGCAATGATACGGCGACCACCGAGATCTACACTCTTTCCCTAC10.32573289902280134Illumina Single End PCR Primer 1 (100% over 40bp)
GAAAAACGAGCGTCGTTGGAAGTGAGACGTGCCGTTCCCATGTACTCTGC10.32573289902280134No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTTGGGTTTAAAACC10.32573289902280134No Hit
ACGCAGAGTACATGGGAGCCGACCTTTACCAACACGACGCTCTTCCCTGC10.32573289902280134No Hit
GCGTTTAGTAAATGCGTTAACTAGGCTTTAAATGACGCAATTCTCCCTGC10.32573289902280134No Hit
GATAATAGTTTTTCAAATTATTCTTCAGGTACAACCTATACCAGACCCAC10.32573289902280134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAAGCAGTGGT10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134No Hit
GTGTAGACTGGGTCCGATCTCCCATGTACTCTGCGTTGATACCACTGCTT10.32573289902280134Clontech SMART CDS Primer II A (96% over 26bp)
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA10.32573289902280134Illumina Single End PCR Primer 1 (100% over 21bp)
ACGCTCTTGCCTCAGATTATGTCGGTACAAGAGATCGGAAGAGAGTCGTG10.32573289902280134No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATTAGGCGACCCCGAGTCTACACTCTGTCCCAAGTTCTGCA10.32573289902280134No Hit
GGCTCATGATGCGGCTCCGGCTTCAAAGGGATGCGCCTGCTTCCAGTGGC10.32573289902280134No Hit
GTATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAGAGTACATG10.32573289902280134Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACATGGGAAGCAGTCCTATCAACGCAGAGTACATGGGAAGCAGTGGTA10.32573289902280134No Hit
GTAGATCTCGGTGGTCGCCTCACCCATGTACTCTGCGTTGATACCACTGC10.32573289902280134Clontech SMART CDS Primer II A (95% over 24bp)
GAATCTACAATGAACTCAAACAAATTTACAAGAAAAAAACAAACAACCCC10.32573289902280134No Hit
TATCAACGCAGAGTACATGGGTAACGCGACACCGAGATCTACACTCTTTC10.32573289902280134Illumina Single End PCR Primer 1 (95% over 22bp)
GGCTGGAGAAAGCGGATCTCTTCGCCGTCCAGCTGCGCGGCGACCGGACG10.32573289902280134No Hit
ACGCTCTTGCCTCAGATTCTGTCGGTACCAGAGATCGGAAGAGCGTCGTG10.32573289902280134No Hit
GTACATGGGAAGCAGTGGTATCAACCCAGAGTACATGGGGCAGTGGTATC10.32573289902280134Clontech SMARTer II A Oligonucleotide (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTGGGTGGTGG10.32573289902280134No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATAC10.32573289902280134No Hit
GTATAAGTACTTAATACCATCTAGAACTCAATCAAAATGAGAGCTTTATT10.32573289902280134No Hit
GTGTAGATGTCGGGTCCCGTACCCCATGTACTCTGCGTTGATACCACTGC10.32573289902280134Clontech SMART CDS Primer II A (95% over 24bp)
CCGGTCACCACGCCGGGCACGTCGGCCAGCAGTTGCGCGTAGGACTCCTG10.32573289902280134No Hit
GTACATGGGAATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACA10.32573289902280134Illumina Single End PCR Primer 1 (100% over 41bp)
TACCACTGCTTCCCATGTACTCTGCGTTCCCATGTACTCTGCGTTGATAC10.32573289902280134No Hit
CACTTAGTCACAGTTTTATGGATAATTGGGATATTCTTTGGTATAGTTGG10.32573289902280134No Hit
CTCGGGGCGCCGATCATCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.32573289902280134No Hit
TCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGGGACAG10.32573289902280134Illumina Single End PCR Primer 1 (100% over 25bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTT10.32573289902280134No Hit
ACGCTCTTCCGATCTCTAAACAGGATGAGTCCGGGCATACCGGACTCATC10.32573289902280134No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
CCCTACACACGCTCTTCCGATCTGTTCTGGTATCGACAAGACCTGGGACA10.32573289902280134No Hit
GAAGAGTGTAATTCGTGGCGCCGTACATCCCATGTGCTCTGCGTTGATAC10.32573289902280134No Hit
GGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134Clontech Universal Primer Mix Long (95% over 23bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT10.32573289902280134No Hit
CTCTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGAGCGTCGTGTAG10.32573289902280134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAATGATACGGC10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGGAAGTGTTTTCAGACTGCAGTCACTCACATTTGGCTCAGATAAATTA10.32573289902280134No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTCATGGAA10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
CACCACGACTACGTGATGAATAACAATTTCGCCTTTGGCGGCGTCAATAC10.32573289902280134No Hit
GAGTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGCAGTGGTATC10.32573289902280134Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAATTCGGTTTAAACCA10.32573289902280134No Hit
GCCCACCTCGGGCTCCCAAAGTGCTGGGATTCCAGGAGTGAGCCACCGCG10.32573289902280134No Hit
GTGTAGGGAAAGAGTGTAATCTCGTGGCCCGTCACCCATGTACTCTGCGT10.32573289902280134No Hit
TGCCATGGCCGGCAGCAACGATCATGCACACGCTGGGCCGCGCCGTGATG10.32573289902280134No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
CCAGGTGCAAGGTGGACTCTTTCTGGATGTTGTAGTCAGACAGGGTACGA10.32573289902280134No Hit
GGTTGAATTAAGCCAATGCTTTTTGGTATGTGTGTTTGCAGATATTGGAA10.32573289902280134No Hit
CGCCCACACTATGATCGTCGTGGGTTCAAGTTCGCGGCCGTCTAGGTCCG10.32573289902280134No Hit
TGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134Clontech Universal Primer Mix Long (95% over 21bp)
GTGTGAACATCGCCACTGTCATTTTTCCAGAACTACTCCATCATCCCAAA10.32573289902280134No Hit
GTGGTATCAACGCAGAGTACATGGGATGTACGGCACCACCGCTGTCTCTT10.32573289902280134No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTG10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT10.32573289902280134Clontech Universal Primer Mix Long (95% over 21bp)
GCAGTAAATACTTGAAAGAAAACGATCTTCTGGCTTAAGTTTTTTCAATA10.32573289902280134No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
CAGTTGTATCAACGCAGAGTACATGGGAATGATACGGCGACCACCGAGAT10.32573289902280134Illumina Single End PCR Primer 1 (100% over 23bp)
CCTCCTCAGTCGACTGAGCGCAGCGGCTACGTGCGGCGCGTCGCGTACGC10.32573289902280134No Hit
GATCGGAAGAGCGTCGTGTAGGGAAGAGTGAGTCTCGGTGTCCTTCCCCA10.32573289902280134Illumina Single End PCR Primer 1 (96% over 25bp)
TCCTTTAACAAGCCCCGTTCTCAAAAGGCTGGGGGTATTTATGTAAGAAC10.32573289902280134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACACTGCGTTTTTACCC10.32573289902280134No Hit
GTCTACACTCTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGA10.32573289902280134Illumina Single End Sequencing Primer (96% over 27bp)
CTCTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGG10.32573289902280134Illumina Single End PCR Primer 1 (100% over 23bp)
ATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
CCTCGGCCTCCCAAAGTGCTGGGATTACAGGCATGAGCCACCGTGCCTGG10.32573289902280134No Hit
ACGCAGAGTACATGGAAGATACACGAGATCTACACTCTTTCCCTACACGA10.32573289902280134Illumina Single End PCR Primer 1 (100% over 27bp)
AGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTAA10.32573289902280134No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGC10.32573289902280134No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134Clontech Universal Primer Mix Long (95% over 22bp)
GCCCGCACCGCACCGGGCCGGAGGTGGTCGTACACCGAGGACTCGGCGAC10.32573289902280134No Hit
GATCTCGGTGGCGCGCTCCCATGTACTCTGCGTTGATACCACTGCTTCCC10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
CCTTTGAGTTGATGAACCAGAAGTGGCACACATGACTTCTGCTCACATCC10.32573289902280134No Hit
GTATCAACGCAGAGTACATGGGTACATGGGAAGCAGTGGTATCAACGCAG10.32573289902280134Clontech Universal Primer Mix Long (95% over 23bp)
GCCTCGGCCAAGCGCCAGGCCGTGACCAACCCCAAGAACACGCTGTACGC10.32573289902280134No Hit
TATCAACGCAGAGTACATGTTAAGCAGTGGTATCAACGCAGAGTACATGG10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACAGGGGAAGCAGTGGTATCAACGCAGTGTACATGGGAAGCAGTGGTA10.32573289902280134Clontech Universal Primer Mix Long (96% over 25bp)
GTATAACGCTGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TAGGGAAAGAGTGTAGATCTCGGTGGTCCCTACTTCCCATGTACTCTGCC10.32573289902280134Illumina Single End PCR Primer 1 (96% over 30bp)
ACGCAGAGTACATGGGAGCGCGCCCCGAGATCTACTTTTCCCTACACCTG10.32573289902280134No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAGGGGCTGT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GCAATGAACAGAGGCAGGAAATGGTTAGTCATGATGATGACTGTCTCTTA10.32573289902280134No Hit
GCCCAGGATGGTTTCCTGCCGGCAAACGAAGCCAAGCACTGGATCAGGGA10.32573289902280134No Hit
TCTCAGGACCGACTGACCCATGTTCAACTGCTGTTCACATGGAACCCTTC10.32573289902280134No Hit
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGGAG10.32573289902280134Illumina Single End PCR Primer 1 (95% over 22bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAATTTTATTTTG10.32573289902280134No Hit
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAGTAATC10.32573289902280134Clontech Universal Primer Mix Long (95% over 22bp)
TATCAACGCAGAGTACATGGGATCGCGACCACCAGTTACCTCTTCCCTAC10.32573289902280134No Hit
CCCATGAAGAAGGCCCCGTCCGGCCCGCTGGTGGCGTCGAACTGGGCCCG10.32573289902280134No Hit
GGAGCAACCGCTGGGCCAGCGCCCCTCGACTTCGCTCGGGACGAGCGGTG10.32573289902280134No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAATCGGCGTTTTTACC10.32573289902280134No Hit
GAGTACATGGGTGAGGCCGAGATCTACACCTTTCCCTACACGGAGCTGCC10.32573289902280134No Hit
AGATGGAGTCTGGCTCTGTTGCCCAGGCTGAAGTGCAGTCCAGTGGCACA10.32573289902280134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAG10.32573289902280134Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT10.32573289902280134No Hit
ATCCTCCTGTTCCGCCGCCGCGGCGAGGAGACCGAGGTCCTGCTCGGCCA10.32573289902280134No Hit
GCTCTGCAACTGCAGAATAGAAACTGATGCGTTCTGCAGAGGAGGCGTCT10.32573289902280134No Hit
GTACATGGGATGAATACGGCACCACCGAGATCTACACTCTTTCCTACACG10.32573289902280134Illumina Single End PCR Primer 1 (96% over 25bp)
CAACTACTTTTCTTCATAATGCATTCTTTAAAAAAAAAAAAAAAGCATGT10.32573289902280134No Hit
GGTTAGGGAAGTGGGTACTGTTTTTATACCACAGAGTAGTGGGTTAGAGA10.32573289902280134No Hit
GTACATGGGTGATACGGCGACCACCGAGATCTACACTCTTTCCTACCTGT10.32573289902280134Illumina Single End PCR Primer 1 (97% over 34bp)
GTAGGAGAATGGATAAACAAGCTGTGGTATATTTATACAATGGAAGAGAA10.32573289902280134No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTATGCGTTTTTACCACAGCTT10.32573289902280134No Hit
GTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGG10.32573289902280134No Hit
GTACATGGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGT10.32573289902280134Clontech Universal Primer Mix Long (96% over 27bp)
GCAGTGGTATCAACGCAGAGTACATGGGGTGACTGGAGTTCAGACGTGTG10.32573289902280134TruSeq Adapter, Index 6 (96% over 26bp)
ACGCAGAGTACATGGGTGGCCGATCTAAACATTTACCAACACGGGGCTGG10.32573289902280134No Hit
TATCAACGCAGAGTACATGGGAAAGCGAGATTACACCTTCCCTACACGAC10.32573289902280134No Hit
ACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAACTCGGGTTT10.32573289902280134No Hit
CCCCAAATGTCTACCATGAGCATGCACTGTTCTTTATAATCAGACAACAA10.32573289902280134No Hit
GCCAGTGATTGCTCGCCCCTTCCCCGTTCTGCCAGTCAGGCATGATCAGC10.32573289902280134No Hit
CTTTACTTCTTTCCAATTTGGATGCCTTTTCTTTTTCTTGCCTAATTGCT10.32573289902280134No Hit
GGTATCAACGCAGAGTACATGGGTGATACGGCGACCACCGAGATCTACAC10.32573289902280134Illumina Single End PCR Primer 1 (100% over 27bp)
TGCCGGCCACGGTCAACCCGGCCTCCAGCGCCAGCGCCCGCACCTGCGCC10.32573289902280134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT10.32573289902280134No Hit
GGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGA10.32573289902280134No Hit
GGAAGCAGTGGTATCAACGAAAAAAAAAAAAAGTACTCTGCGTTGATACC10.32573289902280134No Hit
GATCATGGGCCACCGTGCCGGCTGGATGGCCCTCGGCTCGGGCATCGCTG10.32573289902280134No Hit
GGTGATAGGCGACCACCGAGATCTACACTCTTTCCCTACACGACGCTCTT10.32573289902280134Illumina Single End PCR Primer 1 (97% over 44bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGA10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGCCGAGACTACACTCTTTCCCTACAGACGCTCTTCCGATCTC10.32573289902280134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.32573289902280134No Hit
CCCCCAGAAGTACTTCCGCTCAGAGAAGAAGGAGACGGCAAGCAGGGTGT10.32573289902280134No Hit
NTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTCGTATGCCGT10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACCCAATCTGAACAACAAAAAAATACACTGGGGAAAAAAAAAAGCGCAAA10.32573289902280134No Hit
TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCGGAGTACATGGAAATGATACG10.32573289902280134Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAGTACACTGCGTTGATACCAATTCTTC10.32573289902280134No Hit
GAGTACATGGGAAAGAGTGTAGATCTCGGTGATACGGCGACCACCGAGAT10.32573289902280134Illumina Single End PCR Primer 1 (95% over 24bp)
TCCCACGCCCATGGAGCCTCGCTCACTGCTAGCACAGCAGTCTGAGATGG10.32573289902280134No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTGTTAACACAGATTCCC10.32573289902280134No Hit
CGCCCCCACAGGGCCCGGGACGGGACCGAGGGGCACCGACCGAGGAGATG10.32573289902280134No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGGGGGTTTTTAAAAC10.32573289902280134No Hit
CTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGCGTCGTGTAGGGA10.32573289902280134Illumina Single End PCR Primer 1 (100% over 25bp)
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134Clontech Universal Primer Mix Long (95% over 22bp)
GCGCAGGCTCCTGGAGCAGCTCCTCGGGCAGGAAACCCTGTACGCCAGGG10.32573289902280134No Hit
GTAGAGGGCCATGTTGTCGCGGTTGATGCTGTCGGTGATGACCAGTACCA10.32573289902280134No Hit
GTACATGGGAGCAGTGGTATCAACGCGAGTACATGGGAAGCAGTGGTATC10.32573289902280134No Hit
CCGCTGGTCCCGACTTCAACAGATGGCTGATCCCGCCCGCAGCACTTGGC10.32573289902280134No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATCGGCGTTGAAACCA10.32573289902280134No Hit
GGGACGCCATCCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGCTTCCCATGTACTCTGCGTTGATACCACTG10.32573289902280134Clontech SMART CDS Primer II A (95% over 23bp)
GAAGCAGTGGTATAAACGCAGAGTACATGGGTGTAGGCCCGATCTACCTC10.32573289902280134Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GACCTGCCTTTATTTTATAAAACAACAGAATGAAATGGAAACATCTGAAT10.32573289902280134No Hit
GGGAAGCAGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134Clontech Universal Primer Mix Long (95% over 24bp)
CAACGCAGAGTACATGGGGCAGTGGTATCAACGCAGAGTACATGCTGTCT10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTAT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
TCACTTCCCTACACGACGCTCTTCCGATCTCAGACCATGATGCGGGGACT10.32573289902280134Illumina Single End PCR Primer 1 (100% over 26bp)
CAGGAACAAGCCATGGAGGGTCAAAGAAACTCCTGGTTGAGCTCAAAGTT10.32573289902280134No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
CCTTCTTGCTGCGGTCGCGACCCACTGAATGGGTCTCGTCGTGCTCGACC10.32573289902280134No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTA10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAAGAGCGTCGTGTAGGAAAGAGTGTAATCTCGGTGGTGCGCATCCCATG10.32573289902280134No Hit
GTACATGGGGTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAA10.32573289902280134No Hit
CCACCGAGATCTACACTCTTTCCCTACAGCAACTCCTGTCTCTTATACAC10.32573289902280134Illumina Single End PCR Primer 1 (96% over 28bp)
TCCCTACACGACGCTCGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGA10.32573289902280134No Hit
AGCTCGGTAACGCCAATCTTTCGTGGACTCATGAATCTAATCTTTGCACC10.32573289902280134No Hit
GAGTACATGGGGACGCACCACCGAGATTCCCATGTACTCTGCGTTGATAC10.32573289902280134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGCTGTCTCTTATA10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGATAACGCGCCGAGTCTACACTCTCCCAGCAACTCCAGTCCT10.32573289902280134No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATAA10.32573289902280134No Hit
GTGGGAGATGAGGGCAGCTAGAATAGGGAGAGACTAGAATCAGAGAACGC10.32573289902280134No Hit
AAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
CCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACATCTG10.32573289902280134No Hit
CTGCTGGTACCAGTAGACAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAG10.32573289902280134Illumina Single End PCR Primer 1 (100% over 28bp)
AGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTACATGGGACACCACCGAGATCTACACTCTTTCCCCTGTCTCTTATACA10.32573289902280134Illumina Single End PCR Primer 1 (96% over 25bp)
GGGCTTAGGTTATTCCATTAGCCTCTGGTCAGCCCTTCCTGACTCTACTG10.32573289902280134No Hit
GTACATGGGATGATACGCGACCACCGAGATCTAACTCTTTCCCTACACGA10.32573289902280134No Hit
GTACATGGCAATGATACGGCGACCACCGAGATCTACACTTTTCCCTACAC10.32573289902280134Illumina Single End PCR Primer 1 (96% over 33bp)
CCCTCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA10.32573289902280134Illumina Single End PCR Primer 1 (100% over 21bp)
CTGCTAGTGACTTGCTGGACGAGAAGGAAATCGCTAAAAATTTGATCCAG10.32573289902280134No Hit
GCCGTAACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
TGTGTACAGTCTGTGGGGCCGGTGTGAACCCACTATTTTGTGTCCTTGAG10.32573289902280134No Hit
ATATGGTAGTTGTGTTTGTAGTTTTTTGTTTTGTTTTGTTTTGTTTTTTG10.32573289902280134No Hit
TTGGGCTTCTCACTCATCAGGCTCCCCTTCTAAGCAGTTCGTGGTTAACT10.32573289902280134No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTACGCGGCGTTTATACCA10.32573289902280134No Hit
CTTGGAAACCAGCGATCACCTGCATGCAACTGAAAGTGCCAGGGGGTCAG10.32573289902280134No Hit
GCGCTACGGCGGTGTGTATACCATGGCTCTTGCGTATGCTGGCACCTGTC10.32573289902280134No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GATCTACACTCTTTCCTACAACGACGCTCTTGCCTCAGATTCTGTCGGTA10.32573289902280134No Hit
AGCTGGGACTGATGCGTGAGCAAGATATCAGCACCAAGCGCTTTCGCGAA10.32573289902280134No Hit
AGCAACCAGATGTCGTTGGCCGACATTTCAACCGTCTCATCGCGTTCCCG10.32573289902280134No Hit
CCTTATGGCTTGTGTGCGTGCTGGCGTCGGTCGGGAGGACGCCCATGAGG10.32573289902280134No Hit
GAAAGAAGCCAGAAGTTAAGAAAGTGGAGAAGAAGCGAGAAACATCAATG10.32573289902280134No Hit
CTGCTGGCCGAGGAGCTGGGCGTGCCGCCCACCGGGCAGATGACCCGGCT10.32573289902280134No Hit
CTCGTTTTAGGACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAGGGAA10.32573289902280134Illumina Single End PCR Primer 1 (100% over 26bp)
CTCTAGTACTGTATTAAAGTCAAAATATATTCTAGATTTTTTGTTTTGGC10.32573289902280134No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGTGATACGGCGA10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTGCTTACTCTTACAAAAATCAAACTTCTTCCTCTCCCCTTCCTCTCTC10.32573289902280134No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTATGCGTTTGATACCAC10.32573289902280134No Hit
GAGTACATGGGAATGATACGGCGACCACCGAGATTACACTCTTTCCCTAC10.32573289902280134Illumina Single End PCR Primer 1 (95% over 23bp)
GTCGTAGGGCGCGCGCGGCTCCTCGCACAGCAGCGTGACCGACAACGAGC10.32573289902280134No Hit
GTACATGGGCGTCTTCTACCGATCTCTGGTACCTGTCCCCAGTTTCTGCA10.32573289902280134No Hit
CTACAGGCACATGTCACCACGCCCAGTTAATTACACCTTTTCTTTAAATA10.32573289902280134No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAACTT10.32573289902280134Clontech SMARTer II A Oligonucleotide (95% over 24bp)
CCTTAGACATGCCGTGGTATTTTGTTTTTGTTAGCTCTTTTGCGAACTTT10.32573289902280134No Hit
GTCCATCACCTTTGGTGCTGGCAGCGTGACGACCGTGTCGGGCGCAGGGT10.32573289902280134No Hit
AGTCTCGGTGGTCGCCGTCACCCATGTACTCTGCGTTGATACTGTCTCTT10.32573289902280134No Hit
GACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGC10.32573289902280134No Hit
CTGTGGTACCAGTAGACAGTGAGATCGGAGACGTCGTGTAGGGAAAGATG10.32573289902280134No Hit
GGTGGACCCCACCGCATACGTGGGCGCTGTCCTTCCGGTCTGTCTCTTAT10.32573289902280134No Hit
CTTCACGACCGTGCTCGAGGGCCTGGAGGCCGCGGTGTCGGTGCTGTCCG10.32573289902280134No Hit
GTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGATAGGCGCCCCG10.32573289902280134Clontech SMARTer II A Oligonucleotide (96% over 25bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATTAAAAGGCGTTTATACCA10.32573289902280134No Hit
CACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCATGCTTCCCATGTACTCTGCGTT10.32573289902280134No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT10.32573289902280134Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCGTGACGAGGATCACGGGCACGTCGGGCACGCGCTCGCGGCACTGCGC10.32573289902280134No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACAATGCCTGTCAA10.32573289902280134No Hit
GAGCGTCGTGTAGGGAAAGATGTGTTCGTGCTATCCCATGTACTCTGCGT10.32573289902280134No Hit
GGACATGAGAAAAACCTAAAGCCCTAAAAAAATTACTCCTAAAGCAGGGG10.32573289902280134No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTATCCCTTTTTTGGTACCAAC10.32573289902280134Illumina Single End PCR Primer 1 (100% over 30bp)
TATCAACGCAGAGTACATGGGGAAGCAGTGGTATCAACGCAGAGTACTTT10.32573289902280134Clontech SMART CDS Primer II A (100% over 26bp)
GAGTAGACTCGGTGGTCGCCGTACCCATGTACTCTGCGTTGATACCACTG10.32573289902280134Clontech SMART CDS Primer II A (95% over 23bp)
GGACGGGGACGTAAGCCTGACCAAGTTCTGAGAACTGTTGCCTGGGATAA10.32573289902280134No Hit
GTCCCTGGTGTCCCTGGTCGGGGACGCCACCAAGGCGGCGATCTGGACGG10.32573289902280134No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAATCTGCGTTGAT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)
ATGAGTATCTGCAGATCAAGGTGGGCGGAGACCAGGCGCTGTTCCAGGCG10.32573289902280134No Hit
GTGGTATCAACGCAGAGTACATGGGTGAACGGCGACCACCCAGATCTACA10.32573289902280134Illumina Single End PCR Primer 1 (95% over 22bp)
ACGCTCTTCCGATCTCATGTTTACTGGTATCGGCAGCTCCGTACCAGTAA10.32573289902280134No Hit
GTCTCGGTGTCCCGAGCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.32573289902280134Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)