FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680635

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680635
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA170.822447992259313Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG120.5805515239477503Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG120.5805515239477503Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC110.5321722302854378Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT90.43541364296081275Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT60.29027576197387517Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG60.29027576197387517Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50.24189646831156267Clontech SMART CDS Primer II A (96% over 26bp)
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG40.1935171746492501Clontech Universal Primer Mix Long (96% over 26bp)
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG40.1935171746492501Clontech Universal Primer Mix Long (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT30.14513788098693758Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC30.14513788098693758Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.14513788098693758Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAACTCAAGAAATACGCGGGTGAGCTAAAAAAGAAGTGGGACAAGTTGG30.14513788098693758No Hit
CACCACAGCTTCTTGCTGGATGTCGGGTAGATAGCGGTACATGGTCCTAT30.14513788098693758No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGT30.14513788098693758Illumina Single End PCR Primer 1 (100% over 30bp)
TTGCCCGCCAACGCCGCGCATCGTTAGGAGCCATGGTTTTAGGGTTCATC30.14513788098693758No Hit
AACTAAGCAGAAGGCCCCTGACGGATGGCCTTTTTGCGTTTCTACAAACT30.14513788098693758No Hit
GAATGATGTTAAAGCTGAAACTCCAGTACTTTGGCCACCTCATGCGAAGA30.14513788098693758No Hit
CTCCGTAGGTGAGCCGGTGCACGAACACCTGCTGGCCGAGGTCGGAGAGG30.14513788098693758No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)