FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2067
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA221.0643444605708756Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG110.5321722302854378Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT90.43541364296081275Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG80.3870343492985002Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT60.29027576197387517Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50.24189646831156267Clontech Universal Primer Mix Long (96% over 26bp)
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC50.24189646831156267Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT50.24189646831156267Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG50.24189646831156267Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50.24189646831156267Clontech Universal Primer Mix Long (96% over 26bp)
CGAGCAACCCACACAAGTTCAACTACATGGACAACACCACCAACCAAGGT30.14513788098693758No Hit
GCTCAGTCGTGTCCGACTCTTTGCGACCCCATGAATCGCAGCATGCCAGG30.14513788098693758No Hit
CCACATCTCAGGCCCTCCAGGGCCCAGTCGGCAAGGGGAGGCTCCAGACC30.14513788098693758No Hit
GTGGGACGGCTCGCTGCCGTGGCCGTGGCGGACCGAGGGAACCGACTCCG30.14513788098693758No Hit
TAAAAAAACCCGCCGAAGCGGGTTTTTACGTTATTTGCGGATTAACGATT30.14513788098693758No Hit
TTTCTGCGTTGCTCTGCCCCGCCGATGGGCGGTATCTGCTCTATGCGTGA30.14513788098693758No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG30.14513788098693758Clontech Universal Primer Mix Long (96% over 26bp)
CTGCAGCGCGCCGAGGCCGAAGGCCCGGGCTTTCGTCTACCCACCAACCC30.14513788098693758No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT30.14513788098693758Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT30.14513788098693758Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)