FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680660

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680660
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA64.137931034482759Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC32.0689655172413794Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG21.3793103448275863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG21.3793103448275863Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT21.3793103448275863Clontech Universal Primer Mix Long (96% over 26bp)
CTCGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAGGG21.3793103448275863Illumina Single End PCR Primer 1 (100% over 24bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT21.3793103448275863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21.3793103448275863Clontech SMART CDS Primer II A (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA21.3793103448275863Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC21.3793103448275863Clontech Universal Primer Mix Long (96% over 26bp)
GCATACATGTCCAGCGTCTCGGTCGAGATGCCGGGGCCGCCGCGAGTCAT21.3793103448275863No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT21.3793103448275863Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTAT21.3793103448275863Clontech Universal Primer Mix Long (95% over 21bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT21.3793103448275863Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA21.3793103448275863Clontech Universal Primer Mix Long (95% over 21bp)
GAGTACATGGCAAGCAGTGGTATCAACGCAGAGTACATGGCAAGCAGTGG10.6896551724137931Clontech Universal Primer Mix Long (100% over 26bp)
CAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAA10.6896551724137931No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGATGTAGACGGTGGCGCGTCTTCC10.6896551724137931Illumina Single End PCR Primer 1 (96% over 28bp)
CTCCCGGGCCAAGGTCCTGGCTGAGCTGGAGCCAAAACTCGCCGCTGCGT10.6896551724137931No Hit
CTCTAAGCCTCTTAGAAAACAGTGACAAGAGCTTTGGGACATTTGCTAGG10.6896551724137931No Hit
GTTGTAGTTATCTTCTAATTGTGGCGGAGCAATTGACCATGGTTCAATCT10.6896551724137931No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAACGCGCCGTTAAT10.6896551724137931No Hit
GTACATGGGATGATCGCACCGAGATCTACACTCTTCCTACACGACGCTCT10.6896551724137931Illumina Single End PCR Primer 1 (95% over 21bp)
GATGGATAGGACCGGACGCGACCATCGGCGCGCGCCGCGTGCAAGTGCAC10.6896551724137931No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGACGC10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GGCTGGGGCTGGCACCGCTGCCGCCGCTGTCGCCCGAGCGCAGCGTGTGA10.6896551724137931No Hit
CTTCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCA10.6896551724137931Illumina Single End PCR Primer 1 (100% over 23bp)
TCGGAAGAGCGTCGTGTAGGAAAGCAGTGGTATCAACGCAGAGTACATGG10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AGGGAGAACTGAGGGTGAGTTGGTTTGAGAGAATTGGAATGTCTAATAGA10.6896551724137931No Hit
ACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATCTGCGTTGAAA10.6896551724137931No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG10.6896551724137931No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAAAAATGA10.6896551724137931No Hit
GTTGATACCACTGCTTACCCATGTACTCTGCGTTGATACCACTGCTTACC10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG10.6896551724137931Illumina Single End PCR Primer 1 (100% over 22bp)
GATAAAGATCGAGCGCGCATTGGGCATCAGCTTGCGCACCTGTTCGGCGC10.6896551724137931No Hit
CCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAGGG10.6896551724137931Illumina Single End PCR Primer 1 (100% over 24bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTCTTATACA10.6896551724137931Clontech Universal Primer Mix Long (96% over 25bp)
ACAACGCACAGAAAATTGTAAAAAAAACATACACTACACCTGTCTCTTAT10.6896551724137931No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA10.6896551724137931Clontech Universal Primer Mix Long (95% over 22bp)
GTACATGGGATAACGCGACCACGAGATCTACACTCTTCCCTACACGACGC10.6896551724137931No Hit
TCTCGGGCCCTAATCCCATGTACTCTGCGTTGATACCACCTGTCTCTTAT10.6896551724137931Clontech SMART CDS Primer II A (95% over 21bp)
GGTCCNGAAAAACGAGAGCGTCGTGTAGGAAGAGGTAGATCTCGGGCGCC10.6896551724137931No Hit
GTGAAGCTGATTCTAAGAATATCCAACCCAGATTGGAGTAACTGGAGAGA10.6896551724137931No Hit
GAGTTTACACGACGCTCTTCCGTCTCTGGTACCTGGCAGAACTTGGGACA10.6896551724137931No Hit
CGTTTTTCAGGACCTGTTGGTACCAGATCGGAAGAGCGTCGTGTAGGGAA10.6896551724137931Illumina Single End PCR Primer 1 (100% over 26bp)
CTCTTACCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGG10.6896551724137931Illumina Single End PCR Primer 1 (96% over 30bp)
GTACTCTTGCGTTGATACCAGAAGCAGTGGTATCAACGCAGAGTACATGG10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGCCTGGGGAACGTGGGTCGGCCGGTGGACCGCCGAGGAGAACCGCCAC10.6896551724137931No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GGTCGGAAATCCAGAGGGTGCGGAACTGCAGCGGCGGACGCCCGCGATCG10.6896551724137931No Hit
AAAAAAAAAAAAAAAAAAAAAAGTACTCTGCGTTGATACCCATGATTCCC10.6896551724137931No Hit
ACAACCGCCCGAGCTTCAGTCTGACCGTCACGCGCGAGCGCGCTGGCGCT10.6896551724137931No Hit
TCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGTACCAG10.6896551724137931Illumina Single End PCR Primer 1 (100% over 25bp)
GGGAAGAACGTTCTGGGCAAAGGAAACACCAAATGCAAAGTAGCATGGTG10.6896551724137931No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT10.6896551724137931No Hit
TCCCTACACGACGCTCTTCCGATCTGTCCCAGGTCTTGTCGATACCAGAA10.6896551724137931Illumina Single End PCR Primer 1 (100% over 25bp)
TATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGG10.6896551724137931Clontech Universal Primer Mix Long (96% over 25bp)
GTACATGGGAAGCAGGTGGTATCAACGCAGAGTACTTTTTCTGTCTCTTA10.6896551724137931Clontech SMART CDS Primer II A (100% over 21bp)
GGCGTGCAGTGGTTCGCGGGCATCCAGTTCCTGCTGCTGGTCGGGTGCGC10.6896551724137931No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TGGGACGGCACACCGGATCTACACTCTTTACCAACAGGTCCAGAAAAACG10.6896551724137931No Hit
ATGGGAATGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACGAC10.6896551724137931Illumina Single End PCR Primer 1 (100% over 45bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTTTTTTTTTTTTTTTTGCGTTGATACCACTGCTTCCCATGTACTCT10.6896551724137931Clontech Universal Primer Mix Long (95% over 21bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTATAACAAATC10.6896551724137931No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCTGTCTCTTA10.6896551724137931Clontech SMART CDS Primer II A (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACTGTCTCTTATACACA10.6896551724137931No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCCTGTCTCTTAT10.6896551724137931Clontech Universal Primer Mix Long (95% over 21bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGTACGGCGA10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAGATGGGTTTAT10.6896551724137931No Hit
GGGAAAGAGTGTAGATCTCGGGTGCCGACCCATGTACTCTGCGTTGATAC10.6896551724137931Illumina Single End PCR Primer 1 (95% over 21bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAAAATGCGTTGATACCACTG10.6896551724137931No Hit
GTATCAACGCAGAGTACATGGGTGATACGCGACCACCGATATCTACACTC10.6896551724137931No Hit
CCCGGGATGTGCACGTCGACGACCGTCACGTTGACCTCGGTGACCTCCAT10.6896551724137931No Hit
GAACCGATGTTCCTGCTGATGCTCGGTGCCCTGTCTCTTATACACATCTG10.6896551724137931No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT10.6896551724137931Clontech Universal Primer Mix Long (100% over 23bp)
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GTACTTTTTTTTTTTTTTTTTTTTTTTTTGGTTTTTAACCAATCAACCCA10.6896551724137931No Hit
GCTCTGTGCCGAGGGCGCCGAGGATGATCGGCGTGACGACCAGACCGGAC10.6896551724137931No Hit
AAGCAGTGGTATCAACGCAGAGTACATGGGTGATACGGCGCCACCGAGCT10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCT10.6896551724137931Clontech Universal Primer Mix Long (96% over 25bp)
CTGGAGGAGTGGATCTACACCTCGTTCACCCTCGTTGCGCCGAAGCGGCT10.6896551724137931No Hit
GTCGTGGGTCAGCAACTCGCCACGGCCGATCAGGAAATTGGTTTGACTCA10.6896551724137931No Hit
GTACATGGGTGTCGGGGGGGGCCCCCCGGGGCACCAATATAAAAAACGAC10.6896551724137931No Hit
GAGTACATGGGACGGGCCACCGCGTTAAACACATTACCAACACCGGCATA10.6896551724137931No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGATCGGGACCCAGATCTACATCTT10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GAGTACATGGGCGCCGACCCCTTCCTACACGACGCTCTTCCGATCTCTGG10.6896551724137931Illumina Single End PCR Primer 1 (96% over 25bp)
GCAGAGTACATGGGAATGATACGGCGACCACCGAGATCTACACTCTTTAC10.6896551724137931Illumina Single End PCR Primer 1 (97% over 36bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCCTGTCTCTTATAC10.6896551724137931Clontech Universal Primer Mix Long (96% over 28bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GCATGTACCTTACTACTTACAGAAAATTCCCTGATATTTTCTACTGTTGT10.6896551724137931No Hit
GTGTAGGGAGAGTGTAATCGTCTTCCCATGTACTCTGCGTTGATACCACT10.6896551724137931Clontech SMART CDS Primer II A (95% over 22bp)
TGCACATATCGTCGCGATAGTCCGGCGCGAGGTGCGCGAGATGCTCAGCG10.6896551724137931No Hit
GGTCAGGTCGCGGGCGGCCAGGCGCAGCAGCAGCCGGCGGTACTCCACCC10.6896551724137931No Hit
GAGTTCATCATTAAAGATGAGGAGATCAGCTTCAAGCTCTTCCACGAGTG10.6896551724137931No Hit
GGCCAGCCTTCACTTCCTCTTCGGACAGAGTACCTTCTTCCAGGTACTTG10.6896551724137931No Hit
TCACCATGATGCTGACGCCGATGGTGCTGCCGCTGACCGCACCGCCCTTC10.6896551724137931No Hit
CTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGCGTCGTGTAGGGAA10.6896551724137931No Hit
ATGCAAATGCTGAATGAGGGCATCGTTCCCACTGCGATGCTGGTTGCCAA10.6896551724137931No Hit
GTAGATCTCGGGTCGCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAAGAGTGTAGATCTCGGTGGTCCCTAC10.6896551724137931Illumina Single End PCR Primer 1 (96% over 27bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GGTAGGTATGCGGGCTGATCTTGCCTTTGGGCGGAAGATCAAGGGCGGCC10.6896551724137931No Hit
GCTTTTCAGTAGTGGCTGGGACTGCCAGATGAATAGGCTTGAGGCAGCAT10.6896551724137931No Hit
CGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAAAAAAGGGTTTTT10.6896551724137931No Hit
GCGTCATCATCCTGGGTGACTTCACCGGCATCGAAGAAGGCCAGGAAGTC10.6896551724137931No Hit
AGGTAACTCTGTGGTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTT10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGTACGGCGACCACCGAGATATACACACTTTCCCAAAACG10.6896551724137931Illumina Single End PCR Primer 1 (95% over 24bp)
GAGTACATGGGTGACGGCGACCACCGAGATCTACACACATTACCAACACG10.6896551724137931Illumina Single End PCR Primer 1 (95% over 24bp)
CCGTAGTGCTCCAGGAAGGCTGCATTGAAGCGGCGCACGCTGGCGAAGCC10.6896551724137931No Hit
ACGCAGAGTACATGGGACGGCGACCACCCACAACTACACATTTTTTTATT10.6896551724137931No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTAT10.6896551724137931Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CTGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCACTG10.6896551724137931Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GATGATGGACCTGGCCTGGCGCTCCAGCGTGCCGGTGCTGATCCATCCGC10.6896551724137931No Hit
GAACTACGCGGTGCGCAAATTCCCCGGTTACGGCAAGCTGTCCGGCAAGT10.6896551724137931No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAGTTTAT10.6896551724137931No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGT10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)
GTCGTATCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTG10.6896551724137931Clontech Universal Primer Mix Long (96% over 26bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)