FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680663

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680663
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG190.9854771784232365Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA120.6224066390041494Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT90.46680497925311204Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC80.4149377593360996Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70.3630705394190871Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG70.3630705394190871Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA60.3112033195020747Clontech Universal Primer Mix Long (95% over 21bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG50.2593360995850622Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT40.2074688796680498Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT40.2074688796680498Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AATAAAATAGACTTTTGGAGGATTACTAGATTAGGTGAGGGCTCTGACCA30.15560165975103735No Hit
GTTTCACACCGTCCAGGAGGACTACCGCATCGTCGTCCGCGAGATCACGT30.15560165975103735No Hit
GCATTTATAGCCTGGCTCCCATCCGTCACTGGCTGAGGACTGGTCCCAGG30.15560165975103735No Hit
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA30.15560165975103735Illumina Single End PCR Primer 1 (100% over 21bp)
GCACAGGTTTGTAGCCTAGGAGCACAGGCTACACTTATAGCCTGTAGCCT30.15560165975103735No Hit
ATGTATATCTACACTGCACTATGTACTAGCGTGTTCAGATTACCTGTTAA30.15560165975103735No Hit
CTCCCAAAGTGCTGGGATTACAGGCGTGAGCCACCGCGCCCGGCCGGCAT30.15560165975103735No Hit
ATAAATGAAACAACTAGGTGCTAGCTATCTAATGAATGTTCTCAAAAATC20.1037344398340249No Hit
CTCATAGGTAACTGTGCCTTCTCTCTTCAAAATTATTTTCCATCTTTCTT20.1037344398340249No Hit
TTTTTAAAGAGATGCAATGTCATCAGTGTTTGACTGCTAAGATTAAAATA20.1037344398340249No Hit
CTATAATATACTTTTTTAATACTTATTTTACAGTATTCTACTTATACTAA20.1037344398340249No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.1037344398340249Clontech Universal Primer Mix Long (100% over 23bp)
CTACACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCA20.1037344398340249Illumina Single End PCR Primer 1 (96% over 30bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAT20.1037344398340249Clontech Universal Primer Mix Long (95% over 21bp)
TCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCACT20.1037344398340249No Hit
GTGGTTCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGAGTACATGG20.1037344398340249No Hit
CCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTG20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
GATCTCAGTTCACTGCAATCTCCACCTCTTGCCAACCCTCTTTCTTAATA20.1037344398340249No Hit
ATTATCACCATGTGTCAAAAGAGTTGTGTAACTCATCCTAATAATTTCCA20.1037344398340249No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCTGTCTCTTATACACA20.1037344398340249No Hit
CCTGTCACAAAAACAAAACCAACAACAACAACAACAAAAACCAAAGTATA20.1037344398340249No Hit
CTCGATGCCAAATGGCCGCCTGCCGAAAAGCCCATGATTCCAACTTTGTT20.1037344398340249No Hit
TATCAATCACGTAGAAAGCAATAGCTGCCTTAGGAGATACGTTGTGTTAA20.1037344398340249No Hit
GATGTTACCCAAACACCAGGGTTTGGTTGGGGTTCTAATACTCACTGCAT20.1037344398340249No Hit
GTACATGGGAACCACCATCTACACTCTTCCTACACGACGCTGTCCCCTGT20.1037344398340249No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.1037344398340249Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1037344398340249Clontech Universal Primer Mix Long (95% over 22bp)
GTTCTAGCCCTCAACCAAAGCCAACCCCAGCCAAATGTGAAAAACAAAGC20.1037344398340249No Hit
GATATACAGCGTTATTTCTGAGGGCTCTGTTCTGTTCCATTGATCTATAT20.1037344398340249No Hit
CAAATAGGCTAAAATTAAGGGGTAAGAAAAGATAGATCATGCAAACAGCA20.1037344398340249No Hit
CTCCCAGGCTGGGCTCCGCTAGGCTCCTGTCTCCCCTGCCAGTTAGGCAA20.1037344398340249No Hit
CCTGGAGCGCTCAGACGCAGAGACGTTCAGGTTGGGCATGAAAGAGCTTT20.1037344398340249No Hit
AGTAAAGACATGGTAAATTGCTAACTAAATGTTAGCCTTATTTTAAATTT20.1037344398340249No Hit
GGAATTGTTAGTGTCCTGGGGCTGTTTCTTGACTTGGTCAAATAAAACAT20.1037344398340249No Hit
TGCCATGGTAATCCTGCTCAGTACGAGAGGAACCGCAGGTTCAGACATTT20.1037344398340249No Hit
TTCCATTCGATTCCATTTTATGATGATTCCATGCGATTCCATTAGATGAT20.1037344398340249No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA20.1037344398340249No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGCTGTCTCTTATAC20.1037344398340249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCACAGTTGCACCCCCTCTGCCTGGAACAGTGATTGGCACACAGAAAATA20.1037344398340249No Hit
GCTTTACAGGTTGAGAGCACAGCATATGCAAAGATAAGAAGGCACTGAAG20.1037344398340249No Hit
CAATGAAGGTGAAGGCCGGCGCGCTCGCCGGCCGAGGTGGGATCCCGAGG20.1037344398340249No Hit
GAAAAAGACCCGCACTTATACACTGAAAGGCTCACTGAATACTTCAGAAA20.1037344398340249No Hit
GCCTTTCCATGTTCCAGAGCCTCCAAGGTCCGCTTGCCTGAGGACAATGA20.1037344398340249No Hit
GACCGAGGGTGGGTGGATTGCCTAAGCTCAGGAGTTTGAGAGCAGCCTGG20.1037344398340249No Hit
AGGAAACAGTGCCTAGCATTCGATCTATTTTTGTTTACTGAATCTGTGAC20.1037344398340249No Hit
TAACTCAGGAAACATTCATGTTTATGGTTACAACCATCACATTTTATTGT20.1037344398340249No Hit
CTTGGTAGAGATGGCTAGCCCTAAATTGACAGTATTTATTTTTGTACTAT20.1037344398340249No Hit
GTGGTTGCCAGGTGCTGGGGGTTAGAGGAGGCAGCTGACTACAAAGGCAC20.1037344398340249No Hit
CGGCTGTCCAGCGGCGTGAGGTCGAGCGTCCACTTGGCGGCCGAGCCGGA20.1037344398340249No Hit
GGTGTCGTCGTGGCTGCCGGGCTCGGGTGCGCTCTTCCTCATCTTCGGCG20.1037344398340249No Hit
CCTGTACGCTGGTTGCTCAAAAACTGTAGGAGAGCATTTTTTTTTTTCTA20.1037344398340249No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGT20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTAGATACTGTAATGTAATTACAGGAGCGACAGCCCATCAGGTTTGC20.1037344398340249No Hit
AAGTCAGGGATAACTAAGATGTTTGGCCCCAGCAACTTAATGGATACTGT20.1037344398340249No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
TGAAGCTGATTGGATCTGATGAACAGGAAGTGACAAGTACCTGAGATGTC20.1037344398340249No Hit
GTGGTCTTCCTCTGCTGCGACGCGGCCCGGTTCATCACCGGCGCAGGCAT20.1037344398340249No Hit
AGCTAACAATGTTAATTTGTAAATAAGCCTGCATTCTTAGTGACAGTGTC20.1037344398340249No Hit
AAGGGACTGGGGAAATAAGGGTCTTGGAAACATGCACTGGAATGCCACAC20.1037344398340249No Hit
GCATCTCATTGTGGTTTTGATTTGCATTTCTCTGATGGCCAGTGATGATG20.1037344398340249No Hit
GACTAATGTTTTTGTTTATTTGTTTGTTTGTTTTTAGACAGAGTCTTGCT20.1037344398340249No Hit
GTATCAACGCAGAGTACATGGGGACACTCTTTCCCTACACGACGCTCTTC20.1037344398340249Illumina Single End PCR Primer 1 (96% over 28bp)
CTATCAAATAAAAAAAAATCCGAACCAAAGATAACAACAAAGAGAAAAAT20.1037344398340249No Hit
GGTGTTGAGGAAGAGGTCCCTAGAAGTAGAATTGCTAGTAGAGGACCTGT20.1037344398340249No Hit
CTACTGAGATTTGTTCTATGTGGTTTCTTCCCTAGAAAATCATCTCCATA20.1037344398340249No Hit
ACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA20.1037344398340249Clontech Universal Primer Mix Long (95% over 22bp)
GATCTACACTCTTTCCCTACACGACGCTCTTCGATCTCTGGTACCTGCAG20.1037344398340249Illumina Single End PCR Primer 1 (96% over 32bp)
GTTCTTTTTTCTTTATTTTTTAATACCAAGCGTCTCCTTTTCTAACACTG20.1037344398340249No Hit
GTGTTCATATAGGCATGAAAAGACAAGATTATGTTCTGTACATGTTTTCA20.1037344398340249No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20.1037344398340249Clontech Universal Primer Mix Long (96% over 25bp)
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG20.1037344398340249No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAGTACATGG20.1037344398340249No Hit
GAAATGGCTAGGTGTCTCATGCAGAGGCTGCCTCATGTACAAAACACCTC20.1037344398340249No Hit
GCAATAGGATAAATTGATGGGGGTTTCAGGAAGGAGAAAGTGGTTGATGA20.1037344398340249No Hit
CTAAGGAAGTGCTTGTGCCACCCCTCTCCCAACCACAGGGAGCACAGCTT20.1037344398340249No Hit
ATCTACACTCTTTCCCTACACGACGCTCTTCCGATCTCTGGTACCTGCAG20.1037344398340249Illumina Single End PCR Primer 1 (100% over 37bp)
TCATGGAGCTTATAGTCTAGGAGAGGAGAAAGCTAAATACATTATTTCCA20.1037344398340249No Hit
GCTTGAGCCCAGGAGTTCAAGACCAGCCTGGGAAACATGGCAAAACAATG20.1037344398340249No Hit
ATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAATCCT20.1037344398340249No Hit
GTAAGAGAATACACTTGTGTTGTTTAAAGCCACTTAGTTTGTGGTAATTT20.1037344398340249No Hit
TGTATATAAACTATTAAGTGGAGATTGAGAGATGACTAACAAGTTATTTA20.1037344398340249No Hit
GGTCTGAAACATCCTTTCTCATGGTTTCTTTAGGCCTGTGGTTTGGATTG20.1037344398340249No Hit
CTCTACGTCTGGTACACAGTCCTAAGGGGTTCCCTTCGTCTCCCTCCTGT20.1037344398340249No Hit
ACTGCACCAAAGTGAAGTGCTATAGGTGTGGTGAAACTGGTCATGTAGCC20.1037344398340249No Hit
ACAGATGACAGTGAGAAAACAGAGCTGTATTCATCTGGTAAAATGTGTCA20.1037344398340249No Hit
ATGCTAGCGTGTGCCTGTAATCCCAGATATTCAGGAGGCTGAGGCAGGAG20.1037344398340249No Hit
TAGCTGTTGGTTTCAGAAGAGGCAGCTAAGCATCTGAGAAAAGCTCTCCA20.1037344398340249No Hit
GATCCATCATCCGCAATGTAAAAGGCCCCGTGCGCGAGGGCGACGTGCTC20.1037344398340249No Hit
GCGAGACTCTGTCTCAAAAAAAAAAATAAAATAAATAAATTGCATTTACC20.1037344398340249No Hit
GGGTAGAGGAAATAGGAATAAAGAGATAAGATTTTGAAAAAAGATTTGAC20.1037344398340249No Hit
GATAAGAATAACAATGTCACTGGACTGTTATAAAAGTAGCAGAAGAAAGT20.1037344398340249No Hit
CTCTTAATTTCCACAGTAAAATATGTTCTTATTTATTTAATAAATTTATC20.1037344398340249No Hit
GATAATGAGCAGATGAGAGTTTCTGGATTAGCTTTTCCTATTTTCGATGA20.1037344398340249No Hit
TGAGAAAACTGAATGGAAATGGTTTTAGAGGAAGGAATGGCATATGAAGT20.1037344398340249No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTT20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
CAATAAAATTTAGGAAGACTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1037344398340249No Hit
CCTCTGGGAGAAGTAAAGCAAGACGCCAGCTCGTAGAGCCAAAGTCCCTG20.1037344398340249No Hit
CTTTTTTTTTTTTTACAGTTTGGACTTAATTCTAATTTTTCCTGGCTACA20.1037344398340249No Hit
CTCCTGTGCTTAAGCAATCTTCTCACTTCAGCCTCCTAAAGTGTTGAGAT20.1037344398340249No Hit
GAATATAACTAGAATGTTACCTTATGGACAGTTGCTTACAAGTGTGAATG20.1037344398340249No Hit
GACCTGTTCTCGCTCATGTCCTCACCATCTTCCACCTTCCCCTGTTGACC20.1037344398340249No Hit
TCACCACATGTCCAAGCCCAGCCAAAGCATCTGACCTGGCCCAGGCCCCA20.1037344398340249No Hit
GTTCTACACTTGCTTATTAAGGAGATGAATAGCTTTTCTTAGGGAAGGCC20.1037344398340249No Hit
CTGTAGCCCAGGCCAGAGTGCAGTGGCGCGATCTCAGCTCACTGCAAGCT20.1037344398340249No Hit
TCCACCCTCCTCCTCAGTTCCTATTCCCAGGTCCTTCTGGAACAAGATAA20.1037344398340249No Hit
AAAAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGTGATACGGCGCCACGAGTTACACTCTTTCCCTCACGACGCT20.1037344398340249No Hit
CTCTACACAGCAGTATGCTCATTGTTCTACAACAGCTCTTACAGTGCTCT20.1037344398340249No Hit
ACCAACGGACACATGAAAAAATGCTCATCATCACTGGTCATCAGAAAAAT20.1037344398340249No Hit
CAGATGAACCGATTTTCAATTAAAACCTTATCTACAGGGTGCATAGATAT20.1037344398340249No Hit
GCCTTGGGCCTGTGGTGGACCCTTCTCCTCTGCAAAATGGACATAATAAA20.1037344398340249No Hit
CTTAATCTAGTGACTAAGCGTCTATGTCAAAGAAAAAAATTTTTTCTTTT20.1037344398340249No Hit
ATTCTAAGCAGAGGGGCCAACAGCTGCCAAGACCCTTACAGACAGGTGTG20.1037344398340249No Hit
ACCCTTGAGCACTCTTGGGGGCAGTGTAAAATGGTGCAGCTTCTATGGAA20.1037344398340249No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA20.1037344398340249Clontech Universal Primer Mix Long (95% over 22bp)
GGGTGAGGTTCTCAGCCATGAGGCTGCATCTTTAGTCCCCTTTTGTGGGA20.1037344398340249No Hit
GTTCCATTCCATTCCATTCCATTCCACTCCATTCCATTGCACTGGGGTTG20.1037344398340249No Hit
GTAATGGGGTGTCTGGGTCAAATGGTAGTTCTAGTTCTACATCCCTGAGG20.1037344398340249No Hit
CTCTTCTGACAGACTCAACAGAGAAAATTGTTATTCCTCTTATAGAGGAA20.1037344398340249No Hit
GATTAAAACTATGAAGTTTGGGAAATACCATTTTTTTCTTTATTTTGTGA20.1037344398340249No Hit
ACAGATCAGGTCGATACGGCCGGAAGGGTGTGGCCATTAACTTTGTAAAG20.1037344398340249No Hit
AGCTTGGGCGTCCTGGCCATCACGAGCATGTCACATGCTGCAGAGATTTT20.1037344398340249No Hit
AAAAGAACAAAGGACAAAAAAATTCATCAAATGTGGGCCGGGCATGGTGG20.1037344398340249No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCT20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
TCCTTGTGTTATGTGTATTCAACTGACAGAGTTGAACCTTCCTTTAGACA20.1037344398340249No Hit
GGGATTACAGGCATGAGGCACCGTGCCCGGCCCAGGATTTTGGGAGGCCG20.1037344398340249No Hit
GAGTGAGACTCCATCTCAAAAAAAAAAAAAAAAAAAGGAAAGGAAAAGAA20.1037344398340249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)