FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1928
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG180.9336099585062241Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA120.6224066390041494Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT100.5186721991701244Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA90.46680497925311204Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70.3630705394190871Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT60.3112033195020747Clontech SMART CDS Primer II A (100% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT50.2593360995850622Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50.2593360995850622Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT50.2593360995850622Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT40.2074688796680498Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG40.2074688796680498Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG40.2074688796680498Clontech Universal Primer Mix Long (96% over 26bp)
GAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCA40.2074688796680498Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCCACCGGCGCACGTAGCCGCCGGGCGGCCCATCGACGTAGGACGTGAT30.15560165975103735No Hit
CTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAGAGCGTCGTGTAGGG30.15560165975103735No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30.15560165975103735Clontech Universal Primer Mix Long (96% over 26bp)
ACCCGCCTCGGCTTCCCAAAGTGCTGGGATTACAGGCATGAGCCACTGCG30.15560165975103735No Hit
TACATATTCTTAACAGCTTAAAAGAATCTAGCCAGTCCTGTGCAGTGGCG30.15560165975103735No Hit
CTTCTTAACCAGCCCTGAAAGGGATTCCTGATCCCCATTCATTTACTTTT30.15560165975103735No Hit
TCCCTACACAGTCATGCATCACCATGCCCGGTCAGTTCTGAGAAATGAGT30.15560165975103735No Hit
TTGCAGTCCAGCCTGGGTGACAGAGCAAGACTATGTCTCAAAAAAAAAAA30.15560165975103735No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT30.15560165975103735Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGATTCCGACACGAGATCTACACTCTTTCC20.1037344398340249No Hit
GGTCCTGAAAAACGAGAGCGTCGTGTAGGGAAAGAGTGTAATCTCGTGGG20.1037344398340249Illumina Single End PCR Primer 1 (96% over 27bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.1037344398340249Clontech Universal Primer Mix Long (100% over 23bp)
TAAGTCTGGTTTTTTACTGGAGGGGGTTGGGGGAGGCTCAAAAAGCAGCC20.1037344398340249No Hit
GTCCAGGAGTCGTGCCCTTCCCCTCCCACCTTGAATCATGTTTGCTCCTC20.1037344398340249No Hit
CAGCTACTCGGGAGGCTGAGGCAGGAGAATCACTTGAACTCAGGAGGCAG20.1037344398340249No Hit
CAAATTCTCTCTCTAGCTGCTTCCTCCTTCTTGGTGGTGAGATCCTCAGA20.1037344398340249No Hit
AAACTGATTCAAGCACTAAAAGTAAAAATCATGATCAACAATTCTATTTC20.1037344398340249No Hit
GTGTTTATAAGTTATCCACATAAATTAGTGTGGCAGACCCTACAGTGATC20.1037344398340249No Hit
AATGGTGGCAGAATAGTTTTGAAACAGAACAAGCTATGTGGAAAGTTTAT20.1037344398340249No Hit
AAGTTTATCACTCAGTCAGCTGCAGACATTAAAAAAAAAACTTCGGAAGT20.1037344398340249No Hit
CCCACCAACAGTGTAAAAGTGTTCCTATTTCTCCACATCTTCTCCAGCAT20.1037344398340249No Hit
CGGTGGCGCCGATCTCCCATGTACTCTGCGTTGATACCACTGCTTCCCAT20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
GTATCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
GTGTGACCACTTTTACCATAAAGTTAATGTCTATATTCCAGAGCAAACCA20.1037344398340249No Hit
TATATAAAATGTTATGTATGTATATATAACATATATGTATATATAACATG20.1037344398340249No Hit
GTCCTCTACTAGCAATTCTACTTCTAGGGACCTCTTCCTCAACACCCTGT20.1037344398340249No Hit
GTATCAACGCAGAGTACATGGGTGTACGGCGACCACCGAGATCTACACTC20.1037344398340249Illumina Single End PCR Primer 1 (96% over 27bp)
AGACTTGAAAACTTTAGACATCACCACAGTTAATTTAGCAGCCATGACCA20.1037344398340249No Hit
TTCCAAAACAGGCCCCATTTCTTCAGTGCCTTCTTATCTTTGCATATGCT20.1037344398340249No Hit
CGCCAGGGCTGCCTAGGCGCCCTCCCTGCGTCTCCTTCCGGGCACTGCAA20.1037344398340249No Hit
GAAGGGAGACGGCAGGCCATTTATGAGAAGAAAGTCCATATAAACCCCAT20.1037344398340249No Hit
ACTTGATAATAGTTTTTTTTGTTTGTTTTTTGAGATAAAGTCTTACTCTA20.1037344398340249No Hit
ATTTATGTGTATTTAACAATTATTTTTTCTTGCATAAATTAAGTAATAAC20.1037344398340249No Hit
TTCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA20.1037344398340249Illumina Single End PCR Primer 1 (100% over 21bp)
TTGCTTAAGTCTGAGATTTATGTTACTGTCATGTAAAGTTGGTGATGTAA20.1037344398340249No Hit
CAGTTAGAATGGCAATCATTAAAAAGTCAGGAAACAACAGGTGCTGGAGA20.1037344398340249No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTATAC20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
CTTCTGACCACAGAGCTCTTAAAAATAAGTTTCTCAAATATTTTATCATC20.1037344398340249No Hit
TTCCAAGGCTTTCCAGGACCTCTGTTTCAAGTGCTCCACTCAGCAGAGCA20.1037344398340249No Hit
CGGGTCGCCGTCACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
TTTCTCACCTCCCCTCTCTCCTCAAGCAGAAGGAAGGAATCATTTTTGTT20.1037344398340249No Hit
TGTTATACCTTCCTGATAGATTTTATTCCTTTTTCATTATAAAATATCCC20.1037344398340249No Hit
CTCTTAATCATGGCCTCAGTTCCGAAAACCAACAAAATAGAACCGCGGTC20.1037344398340249No Hit
CTGGAAGGTCTTCAGGGGGCAGTAACACTCATGGAGCTGTCATCTCCCAG20.1037344398340249No Hit
GTACATGGGTGATACGGCGACCACCGAGATCTACACTCTTTCCCTACACG20.1037344398340249Illumina Single End PCR Primer 1 (100% over 41bp)
TTAATGGATAGACTGTCCAAATGACCTCATTTCTAGAACAAGGCTTCTTT20.1037344398340249No Hit
CTGCGGGTTAATGTTTCCACATAGGAATTTTGGAGGAGACACAAACCTTC20.1037344398340249No Hit
CTATATTAGATGTTGGCATCAAAATTATGCTGGTTTGAAATATAAAAAAC20.1037344398340249No Hit
TCTAAGGGAGGTACAGAGATTTATGAAGGACCATACAGGTGTCCCAGCTC20.1037344398340249No Hit
CTTATAATTGTTCACAATAATCTCTTTTAATCCTTTTTACTTCTGTAGAG20.1037344398340249No Hit
CCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCATA20.1037344398340249No Hit
GGCCTGGGAGTTGCCTCTATCTGCCAACCTTTGGCCAGTGAAGAGGATTC20.1037344398340249No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
GTTCTTTATTCTTTAGGGTCCCAAACCAAGGCTTAGGGAAGAGTTTCCAG20.1037344398340249No Hit
CTAAATTAATTCCATACAGGGATGCTCTGTGCATAGTTGGAAAAAGATTA20.1037344398340249No Hit
GAGATGAACACTGGATACACAGGTGAGTGAGGCCTTTCCATGTTTGTAAA20.1037344398340249No Hit
CGTTGCCCCTCTCCCTGAAGGCAGTGAGTAGGAGCAGCAGGCTCTTTGCT20.1037344398340249No Hit
AGTCAGCACTGAACAAACAGAGTTTCCTATGCTTGCGGAAATGCATTTTG20.1037344398340249No Hit
GCTCTAAACAGTCCAATTAAGAAATGGATATTATCGGCCGGGCACAGGCT20.1037344398340249No Hit
CTACTACCCATGGTGTGTGTGTATGTATAATTAAAAACTGTGTTCTTTTT20.1037344398340249No Hit
ATCTTGGCTCACTGCAAGCTCCGCCTCTCGGGTTCACACCATTCTCCTGC20.1037344398340249No Hit
GGCCCTCGCCGAAGATGAGGAAGAGCGCACCCGAGCCCGGCAGCCACGAC20.1037344398340249No Hit
ATTGTATGGTAATCAAAATTTATACCTGGTGGGATAAATGCAAAGATTTT20.1037344398340249No Hit
GGCTAAATCTTAATCTTTTCCCAAAACCCTTCCATTAGGTCCCCTCTAAA20.1037344398340249No Hit
TGACATAATTATCTCCCCACCTCCTAATACCTCCTAATACTATCACCTGG20.1037344398340249No Hit
AGATAATTAAGTTGACAGTAGGGAAAGCAAAAAAAAAAAGCAATCCCTTT20.1037344398340249No Hit
TGGTACAGCAATGAAATTTGTCAATTGATATGGTTCCGTGTTCTTAACAT20.1037344398340249No Hit
CATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAT20.1037344398340249Clontech Universal Primer Mix Long (95% over 22bp)
GTTCGACGAGGCCGCGGCGGCGGCGTTCTGGTTCATCTGGGTCTTGATGC20.1037344398340249No Hit
ACGATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA20.1037344398340249Clontech SMART CDS Primer II A (96% over 26bp)
GTCACAACAACATAGCCCCATAATCACACAAATGGAAACAAAATAATTTT20.1037344398340249No Hit
TCTTAAAACTAGGCCCATCCTCAACTCATAAAGCAGTCATTAAAACCCAT20.1037344398340249No Hit
ATATAAGACGTTAACATTAGAAGGAAGCTGCACTATCTTTGCAACTTTTT20.1037344398340249No Hit
TTATTATCCTCTAGTTGACTAATCCAGTGTATTCTTTCCATTCTTCCTTT20.1037344398340249No Hit
CTTCAAAGCTACTCCTTTTATAAGGCGTTTCCTGGTCCCTCAACTAGAAG20.1037344398340249No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGGCTG20.1037344398340249Clontech Universal Primer Mix Long (96% over 26bp)
TATATATCCTATTGCATCTTCAACAACCTCTCCACTCGGATATCTAATAA20.1037344398340249No Hit
GGTTCACAGGGCTTTAAGAAAGCACAGCTTAGTTTTCAGTAATTTCAAAT20.1037344398340249No Hit
TATCTATCCTTGCCCCGTGGGAATCAGCTCTTTTTATTTCTGCATCTTGA20.1037344398340249No Hit
GTGATTTGATTATGAACGATAAAACTTTAGGCCCGTTACAGGACGCTTTA20.1037344398340249No Hit
TTCGGGTCCTAACACGTGCGCTCGTGCTCCACCTCCCCGGCGCGGCGGGC20.1037344398340249No Hit
AAACAATGAGCAGGGCCTTTATGAAATACAGTGGGCTCCTGTTAATGTAC20.1037344398340249No Hit
CTATAAAGAATTGAGAATAATGGTCTTAAAGAAATTCAGTGAATGATAAG20.1037344398340249No Hit
GTGGCACGCTGAGCTTCGACGCGCCCGACAAGCTGGTGCGCCGCACCTTG20.1037344398340249No Hit
CTGCCCACCTCGGCCTCCCAAAGTGTGGGGTTTACAGGTGTGAGCCACCA20.1037344398340249No Hit
ACCTTAGCTTTACTAAACCAAGACTTTCTCTGGGCCCTAACAATAATCTA20.1037344398340249No Hit
TTATTTTCCTTTCACAACTTATATGGAAATAATGTATTTAGCTTTCTCCT20.1037344398340249No Hit
TGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAC20.1037344398340249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAAATACAAAAATTACTTGGACGTGGTAGCACGCACCTGTAATCCCAGCT20.1037344398340249No Hit
TATCACTGTGTTGATCAGCACACTTTTTCTTACAAGAACATAAACTCTTC20.1037344398340249No Hit
GATTAGAAAATCAACACCATTAAAAATGGACAAAAGATTTAAACAGACAC20.1037344398340249No Hit
CGTTAAAGCAAGCTCAGCCTTCCACTTTATTCATTTCTAAATCATCTTTG20.1037344398340249No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC20.1037344398340249No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGCT20.1037344398340249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTAATTCTCATATAATCTGTTTGAAAGACACACAGGCTGCTGGGGTTC20.1037344398340249No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCTGTCTCTTATACACA20.1037344398340249No Hit
TTCCTGCACATAGCTACATTCCTTCCTTACTATATAAACCTTCAATTATA20.1037344398340249No Hit
GTGCTTAGCCCTGTAGTTGATCTCTTTTTGAAAGGAACAGACTCACTTAT20.1037344398340249No Hit
GTCTCGATCTCCTGACCTTGTCATCCACCTGTCTTGGCCTCCCAAAGTGC20.1037344398340249No Hit
GAGTATGGTTGCAAAGCTGAAACTTAAAGGAATTGACGGAAGGGCACCAC20.1037344398340249No Hit
ACCGTCTGGGTGACAGAGTGAGATTCTTGTCTCAAAAAAAGAAAAAATTT20.1037344398340249No Hit
GTATGGAATGGAATGGAATGGAATGGAATCAACCCGAGTGCAGGGGAATG20.1037344398340249No Hit
GATCATGTAACTCCTCTGCTCAAAACACTCCACTGAATTTCCACCTCACT20.1037344398340249No Hit
GCTGGGCCTTGGGGCCTGGGCCAGGTCAGATGCTTTGGCTGGGCTTGGAC20.1037344398340249No Hit
GATTTGTATTTTCTGTGTCCAGAATGGGCAGAGTCAGTCCCACAAAAGGG20.1037344398340249No Hit
TATTAAAAACTCAGATTCCTGGGTATCATCAGGAGACTTTGATTCAGCAG20.1037344398340249No Hit
TCAATGAATTTGAAGTTGGAAATTATTAGGATGAGTTACACAACTCTTTT20.1037344398340249No Hit
TATCATACCTGCTTGCCACATAGGAAGAAGTTTCGGCTTTTGAGGCTTCC20.1037344398340249No Hit
CTTTCCCTCACGACGCTCTTCCGATCTCATGTTTGATACGGAGCTGCCGA20.1037344398340249No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT20.1037344398340249Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTCTTTGGGTTCCGGGGGGAGTATGGTTGCAAAGCTGAAACTTAAAGGAA20.1037344398340249No Hit
AAAAGACGCTGCCTTCAGTTCTTTCGAATATATACCCAGAAGTGGGATTG20.1037344398340249No Hit
AGTTAAACTCTGTGAGTTGAACGCACACATCACAGAGCAGTTTCTGAGAA20.1037344398340249No Hit
ATAGAATCGAATGGAATCATCGAATGTACTCGAATGGAATAATCATTGAA20.1037344398340249No Hit
CACTTGTATCTATTCCTCTTGGGGAAGACAGAAAGTGAAGGTTTGATAAA20.1037344398340249No Hit
CCCGTAAGTGGCAGCAAGGATGGGATGATGTGATAGAGCGACACATTGGG20.1037344398340249No Hit
TATCAACGCAGAGTACATGGGTATCAACGCAGAGTACATGGGTGTACGGC20.1037344398340249No Hit
CACACGACGCTCTTCCGATCTCTGGTACCTGCAGAACTTGGGACAGGTAT20.1037344398340249Illumina Single End PCR Primer 1 (95% over 21bp)
GCCGTATCGTTCCGCCTGGGCGGGATTCTGACTTAGAGGCGTTCAGTCAT20.1037344398340249No Hit
TTTCAGTATCTCAGAACTTCATCGAAAATAGGAAAAGCTAATCCAGAAAC20.1037344398340249No Hit
ATTTAAAGGAGCATCTCAGTTACGTGTGGCGGACAAAAAAAAGGAGCAGA20.1037344398340249No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1037344398340249No Hit
GCTTCTGCACAGCAAAAGAAACTACCATCAGAGTGAACAGGCAACCTACA20.1037344398340249No Hit
GACGACGACGGGTTTCTTCTTGCTGCTGCTCATGCTGAGTCTCCTTGCTG20.1037344398340249No Hit
AATTATTGCTTTACTATCAATCCATGGACCCTAAAGGAGCTACAGGCATA20.1037344398340249No Hit
TTTACATTTTATTTTTATTTTTATTTTTTTCTCTTTGAGATGGAGCCTCG20.1037344398340249No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)