FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680731

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680731
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA231.9759450171821304Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG141.202749140893471Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT90.7731958762886598Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA80.6872852233676976Clontech Universal Primer Mix Long (95% over 21bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70.6013745704467355Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC60.5154639175257731Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT60.5154639175257731Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG60.5154639175257731Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT50.429553264604811Clontech SMART CDS Primer II A (100% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT50.429553264604811Clontech SMART CDS Primer II A (100% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50.429553264604811Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT50.429553264604811Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC40.3436426116838488Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG40.3436426116838488Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCACG40.3436426116838488No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40.3436426116838488No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTCTGT30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
AGTCAGTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACC30.25773195876288657No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTCTC30.25773195876288657Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAAGCAGTGGTATCAACGCAGAGTACATGGGAAG30.25773195876288657Clontech Universal Primer Mix Long (96% over 27bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
TTCTACTTCCAACCATTTTCCTCTTTCGGATACAACGTTGCAATTCATTC30.25773195876288657No Hit
GGTCAGCCATATCCAAAAGACCACAAGTCATTACTAAGTTGAGCAAAAGA30.25773195876288657No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
CCCCACGAACGTGCGGTGCGTGACGGGCGAGGGGGCGGCCGCCTTTCCAG30.25773195876288657No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
GTGGAGGAGCAGCCTGGAAATGGCAGTCTCTTTCCAGAAGGCAGTGCTCA30.25773195876288657No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTCTGTCCCAAGTTCTGCAGGT30.25773195876288657Illumina Single End PCR Primer 1 (100% over 30bp)
AGGAAGATGCTCGACAGCGTGTGGCCCGCGATGTGGGCCAGCGACGGGTA20.1718213058419244No Hit
GAAGGTAGTCTCGGCCCCCATGTACTCTGCGTTGATACCACTGCTGTCTC20.1718213058419244Clontech SMART CDS Primer II A (96% over 25bp)
GTAATAAAAATCTAACTAACTAACTCATTTATTTCTATTAAAAAGGTATT20.1718213058419244No Hit
GGTATCAACGCAGAGTACATGGGAAGCTGTGGTATCAACGCAAAAAAAAA20.1718213058419244No Hit
GCCCGATACTCCACCTGAGAAGGTCGTCAGCGGTGATCAGGTCGCGACCA20.1718213058419244No Hit
AAACTAGTCAGACTATCCGCACCAAGCTGGTTTAAGATGCTGGGTAGCAT20.1718213058419244No Hit
GAACTGCTCCATCTGCAGCGGCGTGGCCTTGCGGATCAGCGCGCCTATGC20.1718213058419244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTG20.1718213058419244No Hit
ACTCTGTCGCCCAGGCTGGAGTGCAGCAGTGCGATCTCGGCTCACTACAA20.1718213058419244No Hit
GGCAGAATCTGAAGGGAAAAAGAGATGAGAAACATGAAGATTTCTCGGGT20.1718213058419244No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT20.1718213058419244Clontech Universal Primer Mix Long (100% over 23bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGTAAGCAGTGGTA20.1718213058419244Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCTGGGCGGTGGGCATGCCCATCGACGTGAGGAGCGTCGCGTGGATGTCG20.1718213058419244No Hit
CAGTGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.1718213058419244Clontech SMARTer II A Oligonucleotide (100% over 24bp)
GTCGCCCACAGGGCTGGGCGCCGGGTTGTTCAGGCCGGGTTTGATGATCC20.1718213058419244No Hit
GGTTGCACGCGGGTTCGCTGGGACAGCCCCGCTCCCAGATGTAAAGAACG20.1718213058419244No Hit
GTAATTGGAATGAGTCCACTTTAAATCCTTTAACGAGGATCCATTGGAGG20.1718213058419244No Hit
ACCAAGTTCAATGCTGATGAATTTGAAGACATGGTGGCTGAAAAGCGGCT20.1718213058419244No Hit
TCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCC20.1718213058419244No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA20.1718213058419244Clontech Universal Primer Mix Long (95% over 22bp)
CGGCTGGCCCGTCGGCCTCTGGCACGCTCGAACTTCCGGCCCTGTCTCTT20.1718213058419244No Hit
GTTAATAAGAAACCCTAAGCCAAGACCCTCTGCGAACATTCTTTGGGCCT20.1718213058419244No Hit
CTCCTTGGCTGCATCCGCAACCGTCACAACCTGTAGGCACTCCGCGCAAG20.1718213058419244No Hit
GGTATCAACGCAGAGTACATGGGCGCAGGCCGTTTGGCATCTCTGCCCTC20.1718213058419244No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
ATCGATAAGAACGAGCTGAAGCAGGCCCTCTCAGGTTTCGGCTACCGGCT20.1718213058419244No Hit
ATTCAACACTCGTGAGGCCAGGAAGCTATTAAAGGGATGTTTCAAGCTGT20.1718213058419244No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
CTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTT20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAA20.1718213058419244No Hit
CGACGCGGACCTCGCGCAGATCGCGCGCGAGGAGGCCGAGCGCGCCTACG20.1718213058419244No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.1718213058419244Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT20.1718213058419244No Hit
GTACATGGGGTAGAGGCAATGATGGAAGTTAGATTAAGAGACATCAAAGA20.1718213058419244No Hit
GCCCCTTTTCAGACAGTTTTTGATTCGCTCTAGACTTTTTTTTTTTAATA20.1718213058419244No Hit
CCATCCACCGCACCATTGCGAATGGCGAGCTTTATCAGGTCAACTACACG20.1718213058419244No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACCTGTCTCTTATA20.1718213058419244Clontech SMART CDS Primer II A (96% over 26bp)
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA20.1718213058419244Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CGCCCGCCAGCGCCGCGTTGGTCATGGCGAACGGGAACCGCGCCTGCGAG20.1718213058419244No Hit
GCTCCTGCTGCCCTGTGGGCTGTGCCAAGTGTGCCCAGGGCTGCATCTGC20.1718213058419244No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
TCCTAATGGCAGAATTTGGCTGTTTGGCTTCAACTCCTACTTTTTCCAGC20.1718213058419244No Hit
CCTCTACTTTTTAGTTTGATCTATGAAACCAATCACCACATCACAAGCCA20.1718213058419244No Hit
TTCCAAATGTGTATGTCGCTGTGAAGATCTGGAGAAACAAAACAGATTAC20.1718213058419244No Hit
TGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCC20.1718213058419244No Hit
ACCCCGGGCACCAGCAGCGACAGCCGGCAGCGGTCTCCGCTCTCCGACTA20.1718213058419244No Hit
GGCTGGAGTATAGCGGTACAATCATAGCTCCCTGCAGCCTCGAACTCCTG20.1718213058419244No Hit
GATCTACACTTTTTTTTTCGTCGGCAGCGACAGATGTGTAACTGTCTCTT20.1718213058419244No Hit
GTTGTACCCGGCGTTCGCGCGCATAGCTGTGGAACGGCTCGCCATTGATG20.1718213058419244No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
CTGTTGCCCAGGCTGGAGTGCAGTGGCATGATCTCAGCTCACTGCAAGCT20.1718213058419244No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT20.1718213058419244No Hit
TGTGGTACAGGATACCGAAAACCGCACCCATCGCCATCAGCGCGGGAAAC20.1718213058419244No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT20.1718213058419244No Hit
TCTTTAAAGATGACTAGAAAACAGCAAGTAGAAGGGGGAGGAGCCAAGAT20.1718213058419244No Hit
CACGCTGAGCGTGGGAGCTCCGCCACGTGACGGATTCTGTTGCGCAGGAG20.1718213058419244No Hit
TATCAACGCAGAGTACATGGGAAGCAGGGTATCAACGCAGAGTACATGGG20.1718213058419244No Hit
GAGTACATGGGAAGCAGTGTATCAACGCAGAGTACATGGGAAGCAGTGGT20.1718213058419244No Hit
TCTTTATATTTCCTATATGACCGAGCACAAAACTTTTTATCTAACAGGAA20.1718213058419244No Hit
GGCCTGTCGCGCGCCACCCGCGCGCGGCGGCTGAGCGCGATCCGCCAGCT20.1718213058419244No Hit
GTTTTGATCTGATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCCTG20.1718213058419244No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCCTGTC20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTA20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGGCGTCCGTTACACTTTCCCTACACGACG20.1718213058419244No Hit
AGGTTGCAGTGAGCCAAGATTGTGCCACTGCATTCCACCTCGGGTGACAC20.1718213058419244No Hit
GGGTATTTACCACCTCCTCTAGCAGTCTGTTCCAACTTTGGGTGCGTCTT20.1718213058419244No Hit
AACAAGGCCGGGCGCAGTGGCACACGCCTGTAATCCCAGCACTTTGGGAA20.1718213058419244No Hit
GAGCTGAGATCGCGCCACTGCACTCTAGCCCAGGTGACAGAGCAAGACCT20.1718213058419244No Hit
CGCCCGCTCCCAAGATCCAACTACGAGCTTTTTAACTGCAGCAACTTTAA20.1718213058419244No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20.1718213058419244Clontech SMART CDS Primer II A (100% over 26bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA20.1718213058419244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)