FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT191.632302405498282Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA161.3745704467353952Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG100.859106529209622Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG90.7731958762886598Clontech Universal Primer Mix Long (96% over 26bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC80.6872852233676976Clontech Universal Primer Mix Long (96% over 26bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC70.6013745704467355Clontech SMART CDS Primer II A (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA60.5154639175257731Clontech Universal Primer Mix Long (95% over 21bp)
ACCCCCGAGCGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTT60.5154639175257731No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA60.5154639175257731Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTT50.429553264604811Clontech SMART CDS Primer II A (100% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT50.429553264604811Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT50.429553264604811Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG50.429553264604811Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT50.429553264604811Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG50.429553264604811Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50.429553264604811No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG40.3436426116838488Clontech SMARTer II A Oligonucleotide (100% over 23bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.25773195876288657Clontech Universal Primer Mix Long (100% over 23bp)
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA30.25773195876288657Clontech Universal Primer Mix Long (95% over 22bp)
GTACGAGAGGAACCGCAGGTTCAGACATTTGGTGTATGTGCTTGGCTGAG30.25773195876288657No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
CCCCTCCACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGCGC30.25773195876288657No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGT30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.25773195876288657Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
TGTGGAATGTTCTAGCTCTGAGATGTTAACTGAGAAAAGAGAAGTCAAAC30.25773195876288657No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT30.25773195876288657Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATTAAGGTTGTGCAGGTCATCAGCGAACATTCTACTTCCAACCATTTTC30.25773195876288657No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTC30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
CTATCCCCCTCCTCGGTGCCCAGGAATTCTTGTCAGCTTCCTCCGCTTTG30.25773195876288657No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTT30.25773195876288657Clontech Universal Primer Mix Long (96% over 26bp)
GTGGGCCGATCACGAGGTCAGGAGATCGAGATCATCCTGACTAACATGGT20.1718213058419244No Hit
ATACAAAATGTAGCGTTGATAAGATTGAAGCATGTTGAAAGGTAAGTACA20.1718213058419244No Hit
ATACCACTGCTTACCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
CCCTATTAAAAAAAAAAAGTCTAGAGCGAATCAAAAACTGTCTGAAAAGG20.1718213058419244No Hit
CTCAGCCACACATCCATCTTTTCCTTTGGCTACTCTTTTGGGTGAGAAGG20.1718213058419244No Hit
GTACCGAAGAAGATGTCCGGGTCAAAGTAGAAAGATTAAGTGATGAGGAG20.1718213058419244No Hit
GGCCGGAAGTTCGAGCGTGCCAGAGGCCGACGGGCCAGCCGCTGTCTCTT20.1718213058419244No Hit
GCAATATGGTGAAACCCTGTCTCTACCAAAATTACTTAGCCAGGCATGGT20.1718213058419244No Hit
GTGTTTCACCAGTGGATCATCAAACCCGGCCTGAACAACCCGGCGCCCAG20.1718213058419244No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAGCTGTCTCTT20.1718213058419244Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCTTCCGATCTGGTACCAACAGGTCCTGAAAAACGAGAGAGCGTCGTGT20.1718213058419244No Hit
AGCCAGGTATGGTGGCAGGTGCCTGTAGTCCCAGCTACTCAGGAGGCTGA20.1718213058419244No Hit
ATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1718213058419244Clontech Universal Primer Mix Long (95% over 21bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTG20.1718213058419244Clontech SMART CDS Primer II A (100% over 26bp)
GTATAAATCTGAGAATTTCCAAAATTTGTTCTTGAGATTTTCCTTCTTCA20.1718213058419244No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT20.1718213058419244Clontech SMART CDS Primer II A (100% over 26bp)
GTTTGTTGCGATCGCGGGTATCGCCCCCTTGCTGTAGGAAATCCTGTTTC20.1718213058419244No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
GTTCCATCCCGGCCCGGCACGACGTCAGCCGGCGGGTCGACTGCGGCTTC20.1718213058419244No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
AACCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
GCCCTGGGAGTTGCCGCGCAGGCAGCAGTGGATGTGAGGGTGGAAGTCAA20.1718213058419244No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTC20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
GGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTGGTGCATG20.1718213058419244No Hit
GTCTAGCTCAGTTGCCTAGGCTGGAGTGCAGTATTGTGATCTCTGCTCAC20.1718213058419244No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT20.1718213058419244No Hit
CCCCCCTCCACGCGCCCCGCGCGCGCGGGAGGGCGCGTGCCCCGCCGCGC20.1718213058419244No Hit
GCCAGGAGCTGTCTTTGGGGCTGGGGATACAACAGAGAACAAACCAGGTG20.1718213058419244No Hit
ACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAA20.1718213058419244No Hit
GCCCGAGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTCGAGGCC20.1718213058419244No Hit
GTTTTATTATCATTCACATTATTTCATAGAAAAAGGAATATAGCAAACGG20.1718213058419244No Hit
CTAAGAAGTTGGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGC20.1718213058419244No Hit
GTCCGTGCTGGACCAGGTGCTGGTCAAGGAGGCCATCTACGCCACGGGCT20.1718213058419244No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT20.1718213058419244No Hit
AACGCAGAGTACATGGGTGTGCTCTGCTAAGGGGCTTTTGACCGCCTGGC20.1718213058419244No Hit
CCAGTTCGCTGACGCGCATCCCCGTCGCATAGAGGAGTTCGATCGCGCAG20.1718213058419244No Hit
CACCCGAACACGGTCCTTCTCGACAACGGCGACGTGCTGAACGGCAGCCC20.1718213058419244No Hit
GGCAAGTGTCGTGGACTTCGTACTGCTAGGAAGCTCCGTAGTCACCGACG20.1718213058419244No Hit
GTCATAATATAAAATAGCAGCTCCCAGTAACTTCTTCAGGTTTTATCTTC20.1718213058419244No Hit
GTCTTGCTCTGTCACCTGGGCTAGAGTGCAGTGGCGCGATCTCAGCTCCT20.1718213058419244No Hit
CCCTAGTGAGATGAACCCGGTACCTCAGATGGAAATGCAGAAATCACCCG20.1718213058419244No Hit
CAGTGGTATCAACGCAGAGTACATGGGGGCCGAGACTACCTTCCTGTCTC20.1718213058419244Clontech SMARTer II A Oligonucleotide (100% over 22bp)
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA20.1718213058419244No Hit
GTACTTGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
TTACACATCTGTCGCTGCCGACGAAAAAAAAAGTGTAGATCCTGTCTCTT20.1718213058419244No Hit
TCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG20.1718213058419244Illumina Single End PCR Primer 1 (100% over 22bp)
GTTATAAGGACAGTATTGCAAAGACGACTCTCTTCTTCATCATCTGAACT20.1718213058419244No Hit
CACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCT20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
TTCTGAAGTGCAGCGGTGCGATCTCAGCTCACTGCAACATCTGCCTCCCA20.1718213058419244No Hit
CTTGGGCACCGTGCGCAGCCGCAGGGCGTGTGCGTCGTCTTCCGCCGGGG20.1718213058419244No Hit
AAATAGAACCGCGGTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCG20.1718213058419244No Hit
ACTCCAGCCTGGGCGACAGAACGAGACTCTGTCTCAAAAAAAAAAAAAAA20.1718213058419244No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1718213058419244No Hit
CCTGTAATCCTAGCTACTCAGGAGGCTGAGGCACGAGAACTGCTTTGAAC20.1718213058419244No Hit
AGGAAGGACGGGGTATCATGTTCCAATAATTGGGAAAGTTTTTAAGTGAA20.1718213058419244No Hit
GTACATGGGAAGCAGTGGTATCAACGAAAAAAAAAAAAAAAAAAAAAAAA20.1718213058419244No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
GCTGACCCCCTTCGCGGGGGGGATGCGTGCATTTATCAGATCAAAACCTG20.1718213058419244No Hit
GCATTGGACGCCTTGTTCCGGTCTACGAGAAGAAACACCGTCAGGGCCGC20.1718213058419244No Hit
GTACATGGGGTGTGGGCGCACACTACGATGATCGTGTTCAGCGACTTCGC20.1718213058419244No Hit
GGGGGACGCCGACCGCTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGT20.1718213058419244No Hit
ATCAACGCAGAGTACATGGGTCACATGCCCGGCCCTGAAGCAATGGCTAT20.1718213058419244No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
ATGTTGGATCAGGACATCCCAATGGTGCAGCCGCTATTAAAGGTTCGTTT20.1718213058419244No Hit
GGGCGGTGGCTCGCCTCGCGGCGGACCGCCCGCCCGCTCCCAAGATCCAA20.1718213058419244No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA20.1718213058419244Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACATGGGGCCAGGGCCGCCGCATAGCTTGCGCGGCCAGGCCAGCGC20.1718213058419244No Hit
GGCGCTGGTTGAATATGAATCGCCGCTGCCCACCGTAGTGCGCGACATCA20.1718213058419244No Hit
GTACATCCCCAGTCGTGGCCCTTGTCCAGAGGGCCACGACTGGGGATGTA20.1718213058419244No Hit
ATCTGTTTCAATGGCTTCGTCAGTATAGTTCTTCTCCAGGAACTCGCGCA20.1718213058419244No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTATA20.1718213058419244Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG20.1718213058419244No Hit
GGGGGGCTGGGACAGATTGGCAATGGGCCGTCCCCTCTCCCCTTGGTTCT20.1718213058419244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.1718213058419244No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20.1718213058419244Clontech SMART CDS Primer II A (100% over 26bp)
GATCCGTGTCGTAACGTCTGAATATATCCGTCAACCTCTGCAGGACGATG20.1718213058419244No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)