FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2351
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGA140.5954912803062526No Hit
GTATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCG120.5104210974053595No Hit
CTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCCGCCT100.4253509145044662No Hit
CTTTACATCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCAC100.4253509145044662No Hit
GGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTA90.38281582305401957No Hit
GTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCT90.38281582305401957No Hit
GCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGC80.340280731603573No Hit
CGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCC70.2977456401531263No Hit
CACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGA70.2977456401531263No Hit
CCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCC60.25521054870267973No Hit
CATTATTTCATAGAAAAAGGAATATAGCAAACGGTCACGGTCAGGGTTGT50.2126754572522331No Hit
CGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAG50.2126754572522331No Hit
GCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTTGCAGATGCAGC50.2126754572522331No Hit
CTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATGCACCT50.2126754572522331No Hit
GTCACGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTT50.2126754572522331No Hit
GGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTTACATCT50.2126754572522331No Hit
GGTTTGTGGAAGTCGCGTTCTTTACATCTGGGAGCGGGGCTGTCCCAGCA50.2126754572522331No Hit
GGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTGGAAGTCGCGTTCTTT50.2126754572522331No Hit
GGGCAGCAGGAGCAGCAGCTTTTCTTGCAGGAGGTGCATTTGCACTCTTT40.1701403658017865No Hit
TCCTTTGGTCGCTCGCTCCTCTCCTACTTGGATAACTGTGGTAATTCTAG40.1701403658017865No Hit
TCTGGGAGCGGGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCG40.1701403658017865No Hit
GATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTC40.1701403658017865No Hit
AGCACACCCTGAGCAAGAAGGAGCTGAAGGAGCTGATCCAGAAGGAGCTC40.1701403658017865No Hit
GTGCCAAGTGTGCCCAGGGCTGCATCTGCAAAGGGGCGTCGGACAAGTGC40.1701403658017865No Hit
AGGTTATCTAGAGTCACCAAAGCCGCCGGCGCCCGCCCCCCGGCCGGGGC40.1701403658017865No Hit
TCTCCCCCACGCCGCCTCGCCTGCCGCCGCGCTCGTCCCTCCGGGCCGAC40.1701403658017865No Hit
CGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTCCTGCACCTGCG40.1701403658017865No Hit
ACACATCTCTCTCAGCCGCAGAAATGAAGACATCTTTCACCAGCCGCATG40.1701403658017865No Hit
GATAAATGCACGCATCCCCCCCGCGAAGGGGGTCAGCGCCCGTCGGCATG40.1701403658017865No Hit
TCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCC40.1701403658017865No Hit
ATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGAGCAGCA40.1701403658017865No Hit
TTGGAACCCTGATCTACGGGCACTGGAGCATAATGAAGTGGAACCGTGAG40.1701403658017865No Hit
GTACATGGGACCACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCT40.1701403658017865No Hit
AAACCAACCCGGTCAGCCCCTCTCCGGCCCCGGCCGGGGGGCGGGCGCCG40.1701403658017865No Hit
GGGCTGTCCCAGCATCAGGCGCAGCAGCTGCACTTGTCCGACGCCCCTTT40.1701403658017865No Hit
CCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTCCCGACTCTAGCC40.1701403658017865No Hit
GTTTAATGAAGTCTGAAAGGTAAGCGCCATCGTGGTCAGAGATACGGAGG40.1701403658017865No Hit
CCCCCAGGCCCCGCCGTCCTTGCTGTTGCACACGATGGTGTTGGCGTCGC40.1701403658017865No Hit
CCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTCACCC40.1701403658017865No Hit
ACCTCGGGCCGATCGCACGCCCCCCGTGGCGGCGACGACCCATTCGAACG40.1701403658017865No Hit
GAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTG40.1701403658017865No Hit
GTTGATGGCCTCCAGGTTGAGGCGGTTGGGGAACTTGAATTCGTATCCAT30.12760527435133986No Hit
GGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCAAAGAGTGCAAATG30.12760527435133986No Hit
CAGGCGGGCATCTGGCGGCCGAGGCCGGGACTTCGGGGCCCCTCCCGGGC30.12760527435133986No Hit
GCTCAGTGCCATGGGCTTCACTGCGGCGGGAATCGCCTCGTCCTCCATAG30.12760527435133986No Hit
CACCAGAAACATATACACTTTATTGAATGCCATTGTAGAAAAGCGTGTGA30.12760527435133986No Hit
GGTGTGGAGCGCGCTTCAGCTTCGGCCTGAGGCGCTACGCTCACGGGTGC30.12760527435133986No Hit
GACTCTAGCCGCCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCC30.12760527435133986No Hit
GGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATT30.12760527435133986No Hit
CCCGGAGCTGGAGTTATGGCGGCTACGGAGCCGATCTTGGCGGCCACTGG30.12760527435133986No Hit
GCTGGAGTTATGGCGGCTACGGAGCCGATCTTGGCGGCCACTGGGAGTCC30.12760527435133986No Hit
TGTCTAGTCCACGCTCGGAGCCATGCCGTCCAAGGGCCCGCTGCAGTCTG30.12760527435133986No Hit
GTTTTATTCTTAAATGTACAACAGCTCCAAGACAACACTTAATTCCAGCT30.12760527435133986No Hit
GCGTGCAACCTGTCCCGACTCTAGCCGCCTCTTCAGCTCGCCATGGATCC30.12760527435133986No Hit
GGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAGATTAAGCCATGCAT30.12760527435133986No Hit
CTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGTGCCGCCGGTGACTCC30.12760527435133986No Hit
GTGTTCTTCTTTCAGATACAGTAGGATTTATTCAGGATCTTCCGACGACA30.12760527435133986No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG30.12760527435133986No Hit
ACTTTACTCAGATAAAACACAGATGGTAGCAGATAGAGGCCACAAGCTCA30.12760527435133986No Hit
GTGCAGCTGCTGCGCCTGATGCTGGGACAGCCCCGCTCCCAGATGTAAAG30.12760527435133986No Hit
GAGTACATGGGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACC30.12760527435133986No Hit
TTTGCACTCTTTGCATTTGCAGGAGCCGGCGCAGGTGCAGGAGTCACCGG30.12760527435133986No Hit
GCATTTATCAGATCAAAACCAACCCGGTCAGTCCCTCTCCGGCCCCGGCC30.12760527435133986No Hit
GTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGCGTGCAACCTGTC30.12760527435133986No Hit
CGGCAGTGCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTC30.12760527435133986No Hit
CTCCTGCGCCGCCGGTGACTCCTGCACCTGCGCCGGCTCCTGCAAATGCA30.12760527435133986No Hit
ACCCTGCACCTGGTGTTGCGCCTGCGAGGTGGCATTATTGAGCCTTCTCT30.12760527435133986No Hit
GTGGTAAAGCACCCAGGAAGCAACTGGCTACAAAAGCCGCTCGCAAGAGT30.12760527435133986No Hit
GTATCAACGCAGAGTACATGGGACTCTACCCCATCTCCTTGCCGGGTCAG30.12760527435133986No Hit
CCTCTTCAGCTCGCCATGGATCCCAACTGCTCCTGCGCCGCCGGTGACTC30.12760527435133986No Hit
AATACATGCCGACGGGCGCTGACCCCCTTCGCGGGGGGGATGCGTGCATT30.12760527435133986No Hit
TTGCAGATGCAGCCCTGGGCACACTTGGCACAGCCCACAGGGCAGCAGGA30.12760527435133986No Hit
GGGCACACTTGGCACAGCCCACGGGGCAGCAGGAGCAGCAGCTCTTCTTG30.12760527435133986No Hit
GTACAAGATCATGGTTGCTGCCCTGGGCTGGGCCACTGCTGAGCTTATTA30.12760527435133986No Hit
TATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTTTGTG30.12760527435133986No Hit
GGAATATAGCAAACGGTCAGGGTCAGGGTTGTACATAAAAAATCCAGGTT30.12760527435133986No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.12760527435133986No Hit
GGGTAGATTATTCCTTCTTTACTTTCACAATTTTTACGTTGCTGCCAGAA30.12760527435133986No Hit
TATCAACGCAGAGTACATGGGACGCCTCCTCCAAGTCCCAGCGAACCCGC30.12760527435133986No Hit
CGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAGGGTGTTCTTCTCCTG30.12760527435133986No Hit
CCTGGAAGGGGGGTCACCCAAGTCTCCCTGCTGTCATTTCAGGAGGCCGA30.12760527435133986No Hit
TCATAGGACAGAGGAGTGAGTTCTGTCAACAGACAGGCGGCCCCTACTTG30.12760527435133986No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)