FastQCFastQC Report
Tue 10 May 2022
EGAF00003680752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160050
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC8480.5298344267416433No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG7050.4404873477038425No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA6460.40362386754139334No Hit
GTATCGGCTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCAT6420.401124648547329No Hit
GCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAG6230.38925335832552327No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC5480.3423930021868166No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC5210.32552327397688224No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT4960.30990315526398No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA4930.3080287410184317No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT4820.3011558887847548No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT4540.2836613558263043No Hit
GCAATATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGC4520.28241174632927213No Hit
CCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCGCGGTCAAGGCGA4450.2780381130896595No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA4220.26366760387378946No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA4190.26179318962824116No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC4080.2549203373945642No Hit
AGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAATTGCGCT4000.24992189940643547No Hit
CCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCACGTTGCGAGTC3880.24242424242424243No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT3850.24054982817869416No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG3740.23367697594501718No Hit
GTGGAAAGCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCG3560.22243049047172758No Hit
CTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCT3410.21305841924398627No Hit
ATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATT3390.2118088097469541No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA3300.2061855670103093No Hit
CTGCCCTAACTGCGCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGA3280.2049359575132771No Hit
CATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCCCG3180.19868791002811623No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT3170.1980631052796001No Hit
GCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTC3070.19181505779443925No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA2780.17369572008747267No Hit
CTACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCG2740.1711965010934083No Hit
ATTTGAAGCACACCGTTTTTCTTTCTTCTTTCACGGTATCGGCTTCTATG2720.16994689159637613No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG2720.16994689159637613No Hit
ACACATGCTAGCCGCTGGGGAGATTAGCTCGAGTTGCCCCTTTGCCCGAT2680.16744767260231178No Hit
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTA2650.1655732583567635No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA2640.16494845360824742No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT2620.16369884411121524No Hit
GCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACCGTTTTTCTTTCT2620.16369884411121524No Hit
GAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCG2570.16057482036863482No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC2550.15932521087160262No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG2530.15807560137457044No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT2530.15807560137457044No Hit
CTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATGACG2500.1562011871290222No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT2480.15495157763199No Hit
GCTTTCCACTGTCATTCGCCATAGAAGCCGATACCGTGAAAGAAGAAAGA2440.15245235863792564No Hit
CTGTAGCCGCTTGGCAGGACCATTTTTCTGGTCATCGGGTCCAGCGTGGC2410.1505779443923774No Hit
ATCCTGAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTG2380.1487035301468291No Hit
CATCTAGGCATGGAATCTTATGCCAGCTAACGGAACAAGCTTTGTGCCAT2370.14807872539831302No Hit
CCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTTGA2370.14807872539831302No Hit
GCGTACGGCGGGACCTCTGAGAATTGGGATACAGGACCCAAAAGGCTGAA2360.14745392064979693No Hit
TTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGA2310.14432989690721648No Hit
CACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTCAGGTGGT2290.1430802874101843No Hit
CAGTAGGATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGC2250.14058106841611998No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT2240.1399562636676039No Hit
CACCTAAAGTATGCTGCAAACATATGCAGATCCCCTGAGACAGAGTCGCT2160.13495782567947517No Hit
GAATAAATCCTACTGTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGG2150.13433302093095906No Hit
ATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACA2150.13433302093095906No Hit
GGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGG2140.13370821618244297No Hit
GAATTAAATCCGCTTCTTTTACTTCCTCAAGCGTTGAGCGGAATGCAGCA2130.13308341143392688No Hit
GCATATTGCAGCTGAGCCAGCTCAATTTGAAGTTTGCCTTCTCTCGTTCT2110.13183380193689473No Hit
GAATGCAGCAATCAATGTCGTCGGAAGATCCTGAATAAATCCTACTGTAT2100.13120899718837864No Hit
TCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATTTCCCTGC2080.12995938769134646No Hit
GTAATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCC2050.1280849734457982No Hit
GGACAAGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGG2050.1280849734457982No Hit
CTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGCTTCCTTCCGCAG2040.1274601686972821No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA2020.12621055920024993No Hit
CTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACGGGGATAATTGCG2010.12558575445173384No Hit
GTTTTTTCATGTCCCGCATAATCCTCATTTGAAGAATCAATTAAATGCAG1990.12433614495470165No Hit
GGATATCATCTGCTTCAAGCTCCTCAAGCAGCCGAAGCACTGTTTTTTCA1980.12371134020618556No Hit
GTCTTCTTCATAGCTGTCAGCACTCGTCAGGCGGTTGAACCATGTTGATT1970.12308653545766948No Hit
ATCGTTATGTCTCCTTCCCGCGGTCAAGGCGAAACCGCAGCAAACTTCCT1940.12121212121212122No Hit
ATGCACTGCCGCGTCTGACGGGACAAGGGATCAACCTTTCCCGGCAAGGC1930.12058731646360513No Hit
GTATCTGAAAGAAGAACACTGTAGCCGCTTGGCAGGACCATTTTTCTGGT1930.12058731646360513No Hit
GGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATA1920.11996251171508902No Hit
GTATACGGACAACTGATGGACCCACGTTGCGAGTCCAGTAAATCAGGACG1910.11933770696657293No Hit
ATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAAACTTCA1910.11933770696657293No Hit
ATACAAACGCAGGGAAATCAACATGGTTCAACCGCCTGACGAGTGCTGAC1900.11871290221805687No Hit
CTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTC1880.11746329272102468No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA1870.11683848797250859No Hit
GCTATGAAGAAGACCTCCTGTTTGCCACGCTGGACCCGATGACCAGAAAA1870.11683848797250859No Hit
GTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTTGAAGCACACC1850.11558887847547641No Hit
GTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTTTCCAG1850.11558887847547641No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT1840.11496407372696031No Hit
AACATATGCAGATCCCCTGAGACAGAGTCGCTATCGTTATGTCTCCTTCC1820.11371446422992816No Hit
GTTTTGACTGCGGAAGGAAGCCAAGGCCCACCTAAAGTATGCTGCAAACA1820.11371446422992816No Hit
TGACTGGACACTGCATCGGAAGACACTTCGTGCCGACACGAAGCAGCCTC1760.10996563573883161No Hit
GCTGTGTGTTGATTTCATGAATGCGATTTCTGATATGGCGGCGGTCGGTT1760.10996563573883161No Hit
CTTCTATGGCGAATGACAGTGGAAAGCTGTGTGTTGATTTCATGAATGCG1760.10996563573883161No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG1740.10871602624179945No Hit
CCATAGAAGCCGATACCGTGAAAGAAGAAAGAAAAACGGTGTGCTTCAAA1730.10809122149328335No Hit
GTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAATTT1710.10684161199625117No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG1710.10684161199625117No Hit
GTATGTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATCCCCTACGAG1700.10621680724773508No Hit
GAATTGGGGCAAGAGGTCCCGGGGAAACGAAACTGGAAACCGACCGCCGC1680.1049671977507029No Hit
CTCGTGGAAGAGCTTGAAGCTGATCTCCTCATCTTTAATGATGAACTGTC1670.10434239300218683No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT1640.10246797875663854No Hit
GTACATGGGGGAATTCTCCAGATTACTTCCATTTCCGCCCAAGCTGCTCA1640.10246797875663854No Hit
CGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGCAAT1630.10184317400812246No Hit
GTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAGTCAAC1630.10184317400812246No Hit
GGCTGAAAGGGGGCAGTGAAGTCGGACGGACTCAACGCCCCTCTTTACAG1630.10184317400812246No Hit
CCTCTTTACAGCAAATACCAGCGGCCCTGACCCCGGGGAACAGTCGCATG1620.10121836925960637No Hit
GATGATTGACCGCACGCAATTGATATTAGATATTTTTGCAAAGCGGGCGA1620.10121836925960637No Hit
TATCAATTGCGTGCGGTCAATCATCTTCACTTCAATTGCTGTTGCCAATG1610.10059356451109028No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)