FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680799

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680799
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCAAGTACTCTGCGTTGAT26.25Clontech Universal Primer Mix Long (96% over 26bp)
CCGGAAACCCCGCTCGGCAGCTCTGGTCATGTACCCATCCCGACCTGTCT26.25No Hit
GTATCAACGCAGAGTACATGGGGTGGAAGTGAAATCACATGGCTAGGCCG26.25No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA13.125Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTGAGAACCAGCCACCCGGAGCTCAGTTTCTGCTACAGAGCACCTCCTCT13.125No Hit
ACCAGAGCCTGCTGAGCCCCCTTGTCCTGGAGGTGGACCCCAACATCCAG13.125No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCACCACCTCTG13.125No Hit
GTTTTTCACCTGTGAAAATGCTGACAGGACCGGGTGCGGTGGCTCACGCC13.125No Hit
CACCGGAGGCGTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTG13.125No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAACATATCTGCGGTG13.125No Hit
CCGCAGTGCGTGACCGTCAACGTGGTGTCGCCAGGCTGTCTCTTATACAC13.125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTGAT13.125No Hit
CACTCTGTGTGGCTCCTCGGCTTTGATAGAGTGCAAGACGATGACTTGCA13.125No Hit
GAGTCAACTCTTTACCTCATTATTTGCTAATGACACAAATGCAAAGACAT13.125No Hit
ATTCAGCACACGACGCAGCAGCTCGCCGAGTACCTGGACGTGCCGTACGG13.125No Hit
GTATCAACGCAGAGTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAA13.125No Hit
CGTCCATGCTTGATATCGTTTGGTTCATGAACATTAAGTTTCCAGTACAG13.125No Hit
TTTCTCAGCCAGCTGTGCTTTATTGACAATGCGCCCCTCAGGCCTTGACC13.125No Hit
GAATAAAAAACACTGGGAAGGGGTAACCCCCTCACCCCCGGGAGTGGCCC13.125No Hit
AACCAGGACGGAGCAAGCATCCTGGAATCTCTGCTGCAAAAAGGGGGAAT13.125No Hit
TCTAGCAAGCCTCGCTAACCTCGCCTTACCCCCCACTATCTGTCTCTTAT13.125No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTAAACAACC13.125No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTCGACCCTTAA13.125No Hit
CACTTCTTGAAAGCCTGCTGTGTGCCAAGCCTTTCCCCAAAGGAGGATAT13.125No Hit
ATTAGCATCTGTCTCATGAGTTTGACGTTGCGCTGGTGCTGGCGGCGAAG13.125No Hit
CCCGGAAGCTGCCCGGCAGGTCATGGGAATAACGCCGCCGCATCGCCGGT13.125No Hit
ACTAAGCACTCTACTCTCAGTTTACTGCTAAATCCACCTTAGACCCTTAA13.125No Hit
CCCAGATTCAAGCGATTCTCCTGCCTCAGCCTCCCACTGTCTCTTATACA13.125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAT13.125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)