FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680800

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680800
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32
Sequences flagged as poor quality0
Sequence length100
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTAGGAGGGCTGAGAGGGCCCCTGTTAGGGGTCATGGGCTGGGTTTTAC26.25No Hit
GCCTATATACCGCCATCTTCAGCAAACCCTGATGAAGGCTACAAAGTAAG26.25No Hit
GTCGGGATGGGTACATGACCAGAGCTGCCGAGCGGGGTTTCCGGCTGTCT26.25No Hit
GAGCAGGGTCAGTGGAAGCCCCAACGGGAAAGGAAACGCCCCGGGCAAAG13.125No Hit
AGATGGTTGATGCCCGCGCCCTGCCAGCTAAGCTCGCCGTACGGCACGTC13.125No Hit
CTGCTGCTGCAGGAGGCTCCACTTGGTCTCCAGTATCTTGTTCTGCTGCT13.125No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTATAAAAAACC13.125No Hit
TGCGCCGCCGGCTCAACAAGCACGAGGAGAACCTGAAGACCAAGAAGCAG13.125No Hit
TGGGAGGCTGAGGCAGGAGAATCGCTTGAATCTGGGCTGTCTCTTATACA13.125No Hit
ATCAACGCAGAGTACTTGGGAAGCAGTGGTATCAACGCAGAGTACTTGGG13.125Clontech SMART CDS Primer II A (100% over 26bp)
GACCCGGCCTTCCTGGGCATGGCCGTGAGCACCCTGTGTGGCGAGGTGCC13.125No Hit
GTACATGGGAAGCAGTGGTCTCAACGCAGAGTACATGGGAAGCAGTGGTA13.125Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA13.125No Hit
ATAGTGGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGACTGTCTCTTAT13.125No Hit
ATTTTGGAAGGATCCACGTTTCCCATAAGTGGTGGCTGTGGTATCTCTCC13.125No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTAATGTGCGT13.125No Hit
GTACAGCATAAAACAGTCCCAACACATTCTTTTCAAAAGATGTCTTTGCA13.125No Hit
ATGGTATCCAGTGATTCGTTGAAGAGGAGGTGCTCTGTAGCAGAAACTGA13.125No Hit
CCCGAGGCCTGGCTTATGGTGGTGGCCAGGGCCCTCGTCACCCTCGTGCA13.125No Hit
GCCTTTGGTTGTGTTGGAGGGTACATCGCCAGCACGAGTTCTCTGATTGA13.125No Hit
CCCTTCGCCTGTGTGCCTGGAGCCAGTCCCACCACGCTCGCGTTTCCTCC13.125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTTG13.125No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA13.125Clontech Universal Primer Mix Long (95% over 21bp)
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13.125No Hit
CCTGGCGACACCACGTTGACGGTCACGCACTGCGGCTGTCTCTTATACAC13.125No Hit
GACCTCAAGTGATCCGCCTGCCTCAGCCTCCCAAAGTGCTGGGATTACAG13.125No Hit
GTTTGATGCTTAACCCCCCCAATTTCTGTGAGATGGATGGCCAGTGCAAG13.125No Hit
GAGTAAACAAACATTTGAAATGAGGTTTAGCAGTTCTATAAGGAATCCTG13.125No Hit
GGGTCTTCCAATTTAATTTGTTTCTCTTCTAGTGTAATACGAACATCATG13.125No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)