FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTGACCTTCTCTGGCATTCGGGCATTGGCTGTACCCTTCCGCTTACCTA25.88235294117647No Hit
GTATTCCAGTGGACCATCTTGGTTGGCATCAAACTGATGAATGAGAAGCA25.88235294117647No Hit
AAATCGTAGTGCGGCGGGGGCGAGGAGATGCTCCACTCGAGCGTCCGCCC25.88235294117647No Hit
GAAAAACCACTTTCTTGTTGAAAATATCCCTGAAAAGGTCGCTGCTGCTT12.941176470588235No Hit
GCTGGGGTTGTGCAAGCTAGGGACCCATTCCTGTGTAATACAATGTCTGC12.941176470588235No Hit
GATTTCTAGGATAGTCAGTAGAATTAGAATTGTGAAGATGATAAGTGTAG12.941176470588235No Hit
GTATCAACGCAGAGTACATGGGAGGCCACGAGCTACACCTGTCTCTTATA12.941176470588235No Hit
GCTCGGGTCGACGAGATGCCACGCGCAGTTTTCATGCTGTCTCTTATACA12.941176470588235No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCCTGTCTCTTATACA12.941176470588235Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACCTGTCTCTT12.941176470588235No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT12.941176470588235Clontech Universal Primer Mix Long (96% over 26bp)
GGCCGGGTCCCCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCT12.941176470588235No Hit
GGATCTGGGTGCTGGGGCCCGTCCCCGTGAGCCCGCCAGCTCAGGGGCCG12.941176470588235No Hit
TTACTGACTTACGTGATGGGAACTGTACAAGGCAGAGGCTCCCATCTTCC12.941176470588235No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTC12.941176470588235Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCATTCCTCCCCACACTCATCGCCCTTACCACGCTACTCCTACCTATCTC12.941176470588235No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG12.941176470588235Clontech Universal Primer Mix Long (96% over 26bp)
ACACCTTTTTTTTCGTCGGCAGCGTCAGATGTGTAAAAACTGTCTCTTAT12.941176470588235No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG12.941176470588235No Hit
GTGACATACTCCTGGAAGTTCACCTCCTGGTCCTTGTTCCGGTCCAAGTC12.941176470588235No Hit
GAGCAGGTCGGCGTCGGCGGCGTCTAGGGTGTGCACGACGAGGCTGCCCT12.941176470588235No Hit
AGATATCGGTGGTCGCCGTCCCATGTACTCTGCGTTGATACTGTCTCTTA12.941176470588235No Hit
GAGTACATGGGCATACCGGACTCATCCTGTTTAGAGATCGGAAGAGCGTC12.941176470588235No Hit
TGTCCGGGCCGGGTGAGGTTTCCCGTGTTGTGTCAAATTAAGCCGCAGGC12.941176470588235No Hit
GTACATGGGATCGCATTCATGAAATCAACACACAGCTTTCCACTGTCATT12.941176470588235No Hit
GTCCTGTAAGTAGGAGAGTGATATTTGATCAGGAGAACGTGGTTACTAGC12.941176470588235No Hit
GCCTAGGCCTCCCAAAGTGATGGGATTACAGGTGTGAACCATCATGCCCG12.941176470588235No Hit
TCAAGGAGATGATGGAGTTGCAGAGAGACCTGGTGCCAAATCGAAGGATA12.941176470588235No Hit
AGCTAGAGATGGATGAGTGGTGGAGGGGAGTAGAAGGGAAGCAAAGCTGT12.941176470588235No Hit
ATCAACGCAGAGTACATGGGGCAGTGGTCTGTCTCTTATACACATCTCCG12.941176470588235No Hit
TTTTTTGACGGCGGCAACCGACGCGGCGCTGCCGTTTCTCATCTCGAGGG12.941176470588235No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)