FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003680802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34
Sequences flagged as poor quality0
Sequence length100
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCGGCACAAAATCCTCATTCTCCTCATCCATGTGTCCTCCGCTGTGGC25.88235294117647No Hit
CCCTTTGTTTCTCTGGAAGCCCTCATGTTAGGAAATGTCTTCCAAAACGT25.88235294117647No Hit
AAGGAAGCGGGCGACGACCCATGGGGAGGGCGGACGCTCGAGTGGAGCAT25.88235294117647No Hit
CTGGTCATCGGGTCCAGCGTGGCAAACAGGAGGTCTTCTTCATAGCTGTC12.941176470588235No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA12.941176470588235Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGCCATGGCGCTGTCCTGGCTGCAGCGCGTCGAGCTTGCGCTCTTTGCT12.941176470588235No Hit
GCTGAAACAGGTTTTCGGTTTGTTTAACACATTCCCCATTCTGAACCTTT12.941176470588235No Hit
CTCACAGTCGCATCATAATCCTCTCTCAAGGACTTCAAACTCTACTCCCA12.941176470588235No Hit
GTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCACCT12.941176470588235No Hit
TTGGTGCAGACATTGTATTACACAGGAATGGGTCCCTAGCTTGCACAACC12.941176470588235No Hit
AGCCTACTCATTCAACCAATAGCCCTGGCCGTACGCCTAACCGCTAACAT12.941176470588235No Hit
CAGCTACAGAGATTCAAGATGTATGTTCAGCAGTTGGACTTAACGTATTT12.941176470588235No Hit
TAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTCTCACTAGAAAAGTCCAC12.941176470588235No Hit
GGAGTGGAGCCTGCGGCTTAATTTGACACAACACGGGAAACCTCACCCGG12.941176470588235No Hit
ACCACTGCCCCATGTACTCTGCGTTGATCTGTCTCTTATACACATCTGAC12.941176470588235No Hit
TTTTTACACATCTGACGCTGCCGACGAAAAAAAAGGTGTCTGTCTCTTAT12.941176470588235No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGCTGTCTC12.941176470588235Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG12.941176470588235Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CTCCTATACTCTACAACAGTCTGTGTACGCATGCACATCCACAGCTTTGC12.941176470588235No Hit
GATGCCCGGGGCTTGCTATCCGGCCTGCGGCGGCGGAAGCGGCCCCTGAG12.941176470588235No Hit
ATGCAAGTTACTATGGAATGCAAAGGAGTGTCTGACGTCTTTCCTGGGGA12.941176470588235No Hit
CATGAAAACTGCGCGTGGCATCTCGTCGACCCGAGCCTGTCTCTTATACA12.941176470588235No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACCTGTCTCTT12.941176470588235Clontech SMART CDS Primer II A (95% over 21bp)
GTGTAGCTCGTGGCCTCCCATGTACTCTGCGTTGATACCTGTCTCTTATA12.941176470588235No Hit
GCACCCGGTCGATCCCCCTCGAGATGAGAAACGGCAGCGCCGCGTCGGTT12.941176470588235No Hit
TATCAACGCAGAGTACATGGGACGGCGACCACCGATATCTCTGTCTCTTA12.941176470588235No Hit
GTATAAAAGGGGAGATAGGTAGGAGTAGCGTGGTAAGGGCGATGAGTGTG12.941176470588235No Hit
CCCCCTGGATCAGGCCATTGGCCTCCTCGTGGCCATCTTCCACAAGTACT12.941176470588235No Hit
CTTTCGGGCGGGTTGGGAAGCGCTGGAAGATTTTGAACTGAAACACAACC12.941176470588235No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCTGTCTCTTATACA12.941176470588235Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12.941176470588235Clontech SMART CDS Primer II A (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)