FastQCFastQC Report
Tue 10 May 2022
EGAF00003680875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003680875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences638414
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTAGATGCTCCAGTCTCGGCTCAGCCAAACACTGTCAGGGCCCCCAG13230.2072322975373347No Hit
GAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGGCAGAGGCTGG11410.178724150786167No Hit
AGACAGGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCT11110.17402500571729315No Hit
TTATTGAAGAGAGCAAGGCTGGTGGGCGTGGACTCAAAGCATGGCAGCGG10070.15773463614519734No Hit
CCCTCACCCTGGTCACACTGGCTCTCTGCTGCAGCTCCGCTTCTGCAGAG9620.15068591854188662No Hit
GTCCTCCCCGCGGCGGGGTTTTCAGCCCTCGCGGGTGGGCAGCGTCTTGT8990.14081771389725162No Hit
GTCCTGCAGCTCCGCTGCTGCTTTCTTAGTGGGCTCGGCCAAGCCTGTGG8760.13721503601111504No Hit
GGCCAGAGCATCCCCCTCCTCCACCATGAAACTCGCTGTCACCCTCACCC8740.13690175967319013No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8140.12750346953544253No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG7830.12264768629760625No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA7650.11982819925628198No Hit
GTTGGGGATCTTCAGCTTCTAAATGCTAATTACACAGTGAGCTTTGGGCT7540.11810517939769491No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7500.11747862672184506No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC7410.11606888320118292No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT7260.11371931066674604No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA6970.10917680376683468No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA6830.10698386940136025No Hit
GTACATGGGAGCCGCAGGTTCCCCGCGCGCCCCGAGCCCCCGCGCCATGA6810.10667059306343532No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT6640.10400774419107352No Hit
GTACAGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGT6590.1032245533462612No Hit
GGTCTATAGAGCTCACACCCATACTCCCAGCTGGGACCCAGCCTTCCCTG6560.10275463883937383No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG6550.10259800067041137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)