FastQCFastQC Report
Tue 10 May 2022
EGAF00003681166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003681166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1103631
Sequences flagged as poor quality0
Sequence length100
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTGTCCCTGCGGT32680.29611346546082884No Hit
GTGTATGACTACCAGCAGCTGCTGATCGCCTACAAGCCAGCCCCTGGCAC23110.20939969971847475No Hit
TCGCCACACAGGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCC22790.20650017986084115No Hit
GTTCTGGAGATGAGCATTGGGGCGCCGGAAGCCCAGCAACGCCTGGCCCT22680.20550346990977963No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT22160.20079175014112507No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA21280.19281807053263272No Hit
TCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATT20680.18738147079956977No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC20590.18656598083961035No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACCGGCGATGCG18130.16427592193405222No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT17980.1629167720007865No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA17800.1612857920808676No Hit
GTCCTAGATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCA17650.15992664214760188No Hit
TTTCTAGTGAGAGCCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCAT17240.15621163233000884No Hit
ATGTAGTAGAGCGGCACCTCGCCACACAGGGTGCTCACGGCCATGCCCAG16850.15267784250351793No Hit
GCTCATGGGTCTCCACATGAGCCAGAAACACACGGAGATGGTTCTGGAGA16720.15149991256135428No Hit
GCTTAGACGTAGGCACTGCGGGCTTGGCCTGGAAGTTGTGGACTTTTCTA16370.14832856271706757No Hit
CCTCTGCCCACTCCCATCCTCTCCCCAAGCTCCCATTTCTCCAGGGCTTG15840.14352623295286196No Hit
CTCTGGAGCTATCTTCATGATGTAGCAGCAGGTGCCAGGGGCTGGCTTGT15290.13854268319755425No Hit
CCTCCGGAGGGTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTT14650.1327436434822871No Hit
GCATAGCACCTGCAGCAAGATGGATGTGGGCAGCAAAGAGGTCCTGATGG14490.1312938835534703No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA13820.12522301385155002No Hit
GTCATACACCACGAGGCCAGTGGAGCCGATGGAGAAGGTGGCAGTGGTAA13640.12359203393163114No Hit
CCTCAAGACTGGGGATGCTCTCTGGAGCTATCTTCATGATGTAGCAGCAG13150.11915214414962973No Hit
CTCCAGGGCTTGTCCCTGCGGTGTGGGCAGAAGCAGCTTCTTGCCCGTCT13120.11888031416297659No Hit
CTGCTGCCCAGTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGG12840.1163432342875472No Hit
GGTTACCACTGCCACCTTCTCCATCGGCTCCACTGGCCTCGTGGTGTATG12750.11552774432758775No Hit
GCCATGCACCACCACCCACGGAATCGAGAAAGAGCTATCAATCTGTCAAT12710.11516530434538355No Hit
CTCCTGGTGGTGCCCTTCCGTCAATTCCTTTAAGTTTCAGCTTTGCAACC12620.11434981438542412No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12500.11326249443881153No Hit
GGGTGCTCACGGCCATGCCCAGGAAGGCCGGGTCCCCTCCGGAGGGTGCT12350.11190334450554579No Hit
GTTATTGCTCAATCTCGGGTGGCTGAACGCCACTTGTCCCTCTAAGAAGT12200.11054419457228004No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGG12120.10981931460787166No Hit
ATGTAGCAGCAGGTGCCAGGGGCTGGCTTGTAGGCGATCAGCAGCTGCTG11890.10773528471019751No Hit
GCTTTATTCTGTTGTGGCAGGAGTTCCCGGGCCCCTGGGCGCCACCTCTG11780.10673857475913598No Hit
CTGCTACATCATGAAGATAGCTCCAGAGAGCATCCCCAGTCTTGAGGCTC11580.104926374848115No Hit
GCCCCGGACATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGT11550.10465454486146183No Hit
GAATAATGGAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAG11220.10166441500827723No Hit
CATCTAAGGGCATCACAGACCTGTTATTGCTCAATCTCGGGTGGCTGAAC11190.10139258502162407No Hit
GTGCTGAGCCTGCATCTCGCCCCTCTGCCTGGCCCAGCTTAGACGTAGGC11080.10039587507056254No Hit
GTGCACCTGAAACGCCTTCTTATCGTGGTGGTGGTGGTGGTCCTCATCGT11080.10039587507056254No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)