FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003681277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003681277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length100
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCAGGGCGTGGTCATGCTTTCCATTTAATGTTTTCATTCCGGCGCAG25.263157894736842No Hit
TTCATCGTCCTTGGACAGCATTACCTGCACGCCATTGGCGGCCAGCACTT25.263157894736842No Hit
CCCATCAGTTGCCAGACCGGCGCATTCAGCACTTTACCTTTGATATCCCA25.263157894736842No Hit
GAGATGAACAAGAAGTAAAATGGATTATTTAATGGGAAGTTCAGGTGTTG25.263157894736842No Hit
GAATAATGAAGTAAACCAAAACAAGCATTTGAATCATCATTTAGATTTTA12.631578947368421No Hit
GAGTTCGGCGCGGCGAAGGTGTTCCGGCCGTACCGCGACGTGCGCTTCAG12.631578947368421No Hit
TCACCTGTTTGCTGCTGATTTCCGCCTGTGTGGTTGCAGGTATCTGGGGA12.631578947368421No Hit
CTATTCCTGTTGTCCCACAGGTGACTGGTGTGGTGATTGAAGTGACGGAT12.631578947368421No Hit
GCCATGCCGAGCTGGCGGCCAACACTGGCGTGCGCGTGTATTTTTGCGAC12.631578947368421No Hit
GTTGTTACCGGCTCCGCCACAGCCGACGATGACGATCCGAGGATCGCCGA12.631578947368421No Hit
CTTCTAATCTTGGAAGGCGAAGACCCAGATCTGTTATTCTTTGATCCTGT12.631578947368421No Hit
TCGCGAACGCGGATCCCAGGATAAGACCAGTTTGTCGTCGTAGTGCCGAT12.631578947368421No Hit
ATACAAAAATTAGCCGGGCATGGTGGCATGCACCTGTAATACCAGCTACT12.631578947368421No Hit
GGTGACCGCGGTGGCGACATCCGTGATTGCGCGCACCTGGGTGGTCAGGT12.631578947368421No Hit
ACGACACCCACTGGCCGTTGCGATTGACTTCGCGGAGGGCGGCGACGCGC12.631578947368421No Hit
CTTCCTGGTGCCCACCAAGCCATTCATTCATTCATTCATTCATTCATTCA12.631578947368421No Hit
GTTCACCCAGATCTGGAACGACTTCCTGTTCGGCGTGGTGTTCTCCAGCG12.631578947368421No Hit
AGACAGATAAATGGAAGATATTCAGTTTCTCACCTAGCAGCAGTCCTATT12.631578947368421No Hit
TATTTAACGCGCCAATGCGGCGGACGAAGCGGCTGTTAAAGATGGTCATC12.631578947368421No Hit
CGACAGGGCGCCGAACTTCACCGCGTTCGTTGCCAGCTCATGGATTGCCA12.631578947368421No Hit
CGCGCACGTCGGTCGGGGCGCGAAGTTCGGGTGCTGTCTCTTATACACAT12.631578947368421No Hit
GCCTGAGGTGCCCTGTGTGGAGGGAGCCGACAAAGGACTCTCCTGACCTC12.631578947368421No Hit
CAGCAGCTTGTCGGTGTTGAACGCACTGGCAGATTTGCTGACGGCATTCA12.631578947368421No Hit
GGCAGAGACACAGGCAGAGGGAGAAGCAGGCTCCATGCAGGGAGCCTGAC12.631578947368421No Hit
CGCCCATGGAGGCGGCCAGCATCAGGGCGCCCAGGGGCAGCAGGGCCTTG12.631578947368421No Hit
CTGGTGGCGCAGTTGTCGAAACCGACGGGTTGACGCCTGTCTCTTATACA12.631578947368421No Hit
GTGCCGTGTTCTTCACGAACTCTTCGTTACCGGCAGCAAGCGCCCGCGCC12.631578947368421No Hit
GAACGCGACCTGCGTGAGCATTGCGAGCGCTTCGGCTTCGCGCTCGACAA12.631578947368421No Hit
CTCTGGATACAACTGCACAACCAGTTCCTCATCCACCTGTCTCTTATACA12.631578947368421No Hit
GTTGCCGCTCGCATGACGCATACGTATCAAGAAGCTGGTACGCAAAGCGC12.631578947368421No Hit
CTACATGACCCACAACTGTCATTACGCTCGGCGGCGCAGAAACGATGCCA12.631578947368421No Hit
ATGCTGTCTCTTATACACATCTCCGAGCCCACGAGACATCCTGCCTTTGA12.631578947368421No Hit
TCTTTATGCTGTATCGATACACCAAAGAGAAAATAATGAGGGAGCGAAGG12.631578947368421No Hit
CCAATTGAGTGTACCGCTGTCGACATGGAATCTTCTGCGATACAACAATT12.631578947368421No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)