FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003681278

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003681278
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38
Sequences flagged as poor quality0
Sequence length100
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACTAGGCCGTTCGGCTTCTGGAACAAATCGCCTCCATCCCGAGTCTGA25.263157894736842No Hit
GCTCACCGCTTACTACACCGGCCGCCCCGACGCTGCCGTAGCGGCCCAGC25.263157894736842No Hit
TTTGATTGGTCAGGATCCATCGCGCATCAATGACTTATGGCAAGTGATGT25.263157894736842No Hit
GGTTCAAAATCATACAGACCTAATTTTAAGTCCTGGCCTTGCCACCTGAT25.263157894736842No Hit
ACGTCAACCAGCTTGCCGCCAACCTGACCACCCAGGTGCGCGCAATCACG12.631578947368421No Hit
CCTCAGGATCACGACCTGAGCCAAAGGCAGATGCTCTGAACCACCCAGGT12.631578947368421No Hit
CTACTGGATGATTGCCAACATTCCGGTGAGTCTGCGTGTGGGTATTACCA12.631578947368421No Hit
GATCAAAGAATAACAGATCTGGGTCTTCGCCTTCCAAGATTAGAAGCTGT12.631578947368421No Hit
ATGTCAATGGTCATGAAGAAAATGGACGCGGCTGTCCGTGAAGGCGGCGA12.631578947368421No Hit
GCTGACACCGCAATGGTCCCTTTATTCAGCCTCCCTCAAGTTATCCAATT12.631578947368421No Hit
CTTGTTAACATTTGTATTGTATTTTTTTTTCTTTTTTTTTTTTTTTTTAG12.631578947368421No Hit
GGTGCCGGTTTACGCGCACGTTTCTATGATCAATGGCGATGACGGTAAAA12.631578947368421No Hit
GTTATACCTACAGCGGATCCGGCGGCGTCTGGATCAATTGCACCGCGCAC12.631578947368421No Hit
CAGCACGCGTGGTAAACGCCATTCGGTGACCACCATCGTCATACTGCGCG12.631578947368421No Hit
CCGCGTGGCCTTTGCCTGCTGAGTATTCGCCGCCATCTCATCTAACTCTG12.631578947368421No Hit
TCGGCGGGCACGGCGTCTTTGCAGTTGAAGGCTGCATGATCCGCCGGACC12.631578947368421No Hit
GTTAAACCTGCACCATAAACTTATTCTTTATGTCAGCGTAACTTACTCAC12.631578947368421No Hit
GGCAGATACTGACGCAACAGGCCATTGGTGTTCTCGCAAGTGCCGCGCTG12.631578947368421No Hit
GTTCTCATTCTTAAGCGCCGGACCGTTTGTTTATATTGAAATTAACCACG12.631578947368421No Hit
CCACGCGCCATCCGCACATCGTCCCCCCTGCACCCCATCGATTGAGGCCA12.631578947368421No Hit
ATGTTCCGCCGCTCGCGTATCGCAGTCGTGCTGTTTGCCCTGCCTCTCGT12.631578947368421No Hit
GAATCGAACCAGTGAGGTTTTGCGCAATCATGCAGCCAAGAATGGCCTGG12.631578947368421No Hit
GCGTCAACCCGTCGGTTTCGACAACTGCGCCACCAGCTGTCTCTTATACA12.631578947368421No Hit
GGCCCCCCGGTGGCGCTGCGGCCCAAGGCGGCGGAGACGCTGGCGCTGGC12.631578947368421No Hit
CCCCTTGACCGCGCCGGCGGTCAGCCCGCGCACGAAATACTTGCCCGCAA12.631578947368421No Hit
GTGACGACCATGTCCCAGTCGCCTCCCGCTTCCCCCCGCAGCGACAGGAC12.631578947368421No Hit
TTTCAAACCCATTAGTGCATTTTGAAATCAGTTTAGTGGGACGCCTAGGT12.631578947368421No Hit
CACCCGAACTTCGCGCCCCGACCGACGTGCGCGCTGTCTCTTATACACAT12.631578947368421No Hit
GCTGTACCCAAAGCCAGAGTACACCAAGCCCAAACCATGTGAGCCAGTAG12.631578947368421No Hit
GTGGATGAGGAACTGGTTGTGCAGTTGTATCCAGAGCTGTCTCTTATACA12.631578947368421No Hit
GTCACAGAGAGAGAAAAAGAGAAAGAGAGGCAGAGGGAGAAGCAGGCTCC12.631578947368421No Hit
CAGCCGCCGCGCCTGGTCGGTGGTCAGCCCGTGCGCGCCGCCCGCGACCA12.631578947368421No Hit
ATGCAGGACATCGTTCGCGACGCGCTCGATAACGCCGAGGCCGAACAGGA12.631578947368421No Hit
CATCTGTCTCTTATACACATCTGACGCTGCCGACGATCCCTCTCTCTGGT12.631578947368421No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)