FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003681281

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003681281
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA181.7241379310344827Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT161.532567049808429Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG90.8620689655172413Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG90.8620689655172413Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG70.6704980842911877Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA50.4789272030651341Clontech Universal Primer Mix Long (95% over 21bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC50.4789272030651341Clontech SMART CDS Primer II A (96% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT50.4789272030651341Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50.4789272030651341Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC50.4789272030651341Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTCATAGCGACGTCGCTTTTTGATCCTTCGATGTCGGCTCTTCCTAT50.4789272030651341No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.28735632183908044Clontech Universal Primer Mix Long (100% over 23bp)
CCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCA30.28735632183908044No Hit
CTCTTACTTTTAACCAGTGAAATTGACCTGCCCGTGAAGAGGCGGGCATA30.28735632183908044No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30.28735632183908044Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGCTGTCTCTTAT30.28735632183908044Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC30.28735632183908044Clontech Universal Primer Mix Long (96% over 26bp)
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT30.28735632183908044Clontech Universal Primer Mix Long (96% over 26bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.28735632183908044Clontech Universal Primer Mix Long (96% over 26bp)
CTTCGGGCGCGCACAGGCGCGCCTGACGTTCGATGTGCCGCCAGGGCTGA30.28735632183908044No Hit
GCCTGGCGAACATGGTGAAACCCTGTCTCTACTAAAAATACAAAAAAATT30.28735632183908044No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG30.28735632183908044Clontech Universal Primer Mix Long (96% over 26bp)
TCCTACACGACGCTGTCCCAAGTTCTGCAGGTACCAGAGATCGGAAGAGC20.19157088122605362No Hit
GGGAGGGTCGGCTGCACGTCCGCCTCGTCGCGCTGTTCTCCGTCGTGGCG20.19157088122605362No Hit
GCATTCCCGTTAGCAGGGGCGTCTCTTCCATTTTCTGCCTCTTCCACAAC20.19157088122605362No Hit
GAGTACATTTATGAGCTCTTAAACTCCCTTATTGACATACTGTATTTTTA20.19157088122605362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG20.19157088122605362No Hit
CCCCAGGCCGCGCCATGGTGAAGGTGACGTTCAACTCCGCTCTGGCCCAG20.19157088122605362No Hit
GTATCAACGCAGAGTACATGGGCAAGCAGAGACGGCATACGAGCTGTCTC20.19157088122605362No Hit
ATGTTATTCAGTGGTGCGGGCCTTTGGGAGGTGACTACATCACTAGAATG20.19157088122605362No Hit
CTACCATGGTGACCACGGGTGACGGGGAATCAGGGTTCGATTCCGGAGAG20.19157088122605362No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
GTGAAAATGGGACGGGATAAAGTCCGAGAAATGTTTATGAAGAATGCCCA20.19157088122605362No Hit
GAAAGCTGCTTACCTCACAAAGAGGGAAACTAAAACTACCACAAATGTGA20.19157088122605362No Hit
ACACTTTTTTTTTCGTCGGCAGCGTCAGATGTGTATAAAAAAAAAAAAAA20.19157088122605362No Hit
CTCTTTCCCTACACGACGCTCTTCCGATCTCAGTCCCCGCATCATGGTCT20.19157088122605362Illumina Single End PCR Primer 1 (100% over 30bp)
CACTGTGCTTACAGGGCCTACTGGGTAAGTGAGGCTGTTTTGAGATATAG20.19157088122605362No Hit
GGACCAGCCTGAGGAAACCAAATTTGCTCCCCTAACCAATCTCAAAGGAT20.19157088122605362No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG20.19157088122605362Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.19157088122605362Clontech Universal Primer Mix Long (95% over 22bp)
CTCAAAGTGTTTTGATTATAAGAGGCCAATAAGGATTGGGACAAGTGGAA20.19157088122605362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAC20.19157088122605362No Hit
CCCTACACGACGCTCTTCCGATCTCTGGTACCGACAGAATCTGAGGCAAG20.19157088122605362Illumina Single End PCR Primer 1 (100% over 24bp)
GTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACA20.19157088122605362Clontech Universal Primer Mix Long (96% over 25bp)
CTACATCGCCGTGGAGTACGTAGACGACACGCAATTCCTGCGGTTCGACA20.19157088122605362No Hit
ATAGAGCTCTCACCCCAGCATCCACCTTCTCACTTGCTTCTCAAAGGCAA20.19157088122605362No Hit
CCTATATATGTAGCGGCGTCAGCCCTGTTCCGTTTTTGTGTGACGCTGGT20.19157088122605362No Hit
GTGAAAGCGGGGCCTCACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGA20.19157088122605362No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAACCTGTAAATCTCGTATG20.19157088122605362No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
GTACTGAGCAGGATTACCATGGCAACAACACATCATCAGTAGGGTAAAAC20.19157088122605362No Hit
CAATTCTCCTGCCTCAGCCTCCTGAGCAGCTGGGACTACAGGCGCCTGCC20.19157088122605362No Hit
GAGTAGTAAGTACTGAGTGCCTATTTGAAGTGGGTAATCATGAATTTGGG20.19157088122605362No Hit
GTTTTACACGCAAACCGGCTGCGGATCAGCCTGCGGCGCCCACGGGCTGC20.19157088122605362No Hit
GCATATTTTCTTTGGAGAAATGTCTATCCAAGTCTTTTGCCCATTTCTGA20.19157088122605362No Hit
CTTGAATTCCTGACCTCAGGTGATCTGCCCGCTTTGGCCTCCCAAAGTGC20.19157088122605362No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
ATATTCTCCCTGTTATTGATTTCTAGCTTCATACCATTTCAGTTGGAAAA20.19157088122605362No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
GAGTATATTTGGGTTCTCTATTGCTCCTTGAGTAAATTTTGGTAGTTTGC20.19157088122605362No Hit
CTAATACACCCCTCTTTACAAAGAGAGAAACTAAAGCACATAAAAGTTAA20.19157088122605362No Hit
GGGTAGGGCCGGTCCTCGAAACCCAGGCGCCCCCGCGCGTCGCATTCACA20.19157088122605362No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
GCTCTAAAGCAGAAACTTTTATTCCTTCTTCCCTTTGTATCAACTCTTCC20.19157088122605362No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
CGCTTTGGGTGTCGTAGAAGCTCAACTCGTAGTCGAGCACCACGTCGGCA20.19157088122605362No Hit
AATCCCAGCACTTTGGAAGGCCGAGGTGGGTGAATCACCTGATGTTGGGA20.19157088122605362No Hit
AGATATCAGTATCCAAGTGCAAGAAGGTTACAAAACACCAAGCAGATTTA20.19157088122605362No Hit
GGTTTGTGCCGTGCTGCTTTCCAAATAGGTATTGAATTTATGCATTAGTC20.19157088122605362No Hit
ACATAGACATCTTAACATGAGTGAGGTCATCAGCTTCAGTGCAAGGGGAA20.19157088122605362No Hit
GCTCCACGGGAGGTTTCTGTCCTCCCTGAGCTCGCCTTAGGACCTGTCTC20.19157088122605362No Hit
GAGTACATGGGCCCCTCTACTCTTTCCCTACACGACGCTCTTGCCTCAGA20.19157088122605362Illumina Single End Sequencing Primer (96% over 26bp)
CTAGAAGATGAAGTATATGAAACAGTTGTAGATATCTCTGAAGAAGATAG20.19157088122605362No Hit
ACCCACTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACT20.19157088122605362No Hit
GCCTGGGGGAGAGGGATGGTGCGTTGCCTTCCGCCCACCCCCACTATCCA20.19157088122605362No Hit
CCCAAAGACTTTGGTTTCCCGGAAGCTGCCCGGCGGGTCATGGGAATAAC20.19157088122605362No Hit
GGAATGGACTGCAATAGAAAGGATTCGAATGGAATGAAAAAGAATTGAAT20.19157088122605362No Hit
GAATAGATTTAAGCCTTTATGCAGCAATTCCTTAAAAACAAGCTGTGTAT20.19157088122605362No Hit
CACTCTTTCCCTACACGACGCTCTTGCCTCAGATTCTGTCGGTACCAGAG20.19157088122605362Illumina Single End PCR Primer 1 (96% over 27bp)
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG20.19157088122605362Clontech SMART CDS Primer II A (96% over 26bp)
GGAGTGGGTGGAGGCTGTCAGACCCGAAAAGAGGACGGAGAGAAGGGAAC20.19157088122605362No Hit
TGTCCTGGTTGGCAAAGACCGGTCAAGTTTTTACGTGAATGGGCTGACAC20.19157088122605362No Hit
GTATGATTGGTGTTCCACCTGCTCCTCCTCTTCAGAGTCTGACAACGAGG20.19157088122605362No Hit
GTCAGAAAAGTTACCACAGGGATAACTGGCTTGTGGCGGCCAAGCGTTCA20.19157088122605362No Hit
TCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGA20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTTTTTTTT20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
CCCTTCAGCGGCGGCATTCCCGCCAGGTGGGACGAGAGCCTTAGCCACAG20.19157088122605362No Hit
GTGTATGTGCTTGGCTGAGGAGCCAATGGGGCGAAGCTACCATCTGTGGG20.19157088122605362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.19157088122605362No Hit
GTGTAGATCTCGGTGGTCGCCGTACATTCCCATGTACTCTGCGTTGATAC20.19157088122605362Illumina Single End PCR Primer 1 (95% over 24bp)
TTAATATATGTATCTATCTATAATTTCGCTACTAGATATTTCTACTCCCT20.19157088122605362No Hit
GCAAGCAGGAGTATGACGAGTCCGGCCCCTCCATCGTCCACCGCAAATGC20.19157088122605362No Hit
CGCGACGCTGGCGCTGGCGGCCTTCCACGCGCGCTACCGAGACGTCCAGG20.19157088122605362No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTT20.19157088122605362Clontech SMART CDS Primer II A (100% over 26bp)
GGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATCCTTTA20.19157088122605362No Hit
GAAGAAATTAGAGAAGCATTCCGTGTGTTTGATAAGGATGGCAATGGCTA20.19157088122605362No Hit
CGGTCTCCTCCGTCAGCAGGGTGGGCACCCAGAGCGACTCCTGTCTCTTA20.19157088122605362No Hit
CATGAGATCTGATTGGTTTATCAAGGTTTCCACTTTTGCTTCTTCCTCAT20.19157088122605362No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)