FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003681282

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003681282
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1044
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT161.532567049808429Clontech Universal Primer Mix Long (96% over 26bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA151.4367816091954022Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG141.3409961685823755Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG80.7662835249042145Clontech Universal Primer Mix Long (96% over 26bp)
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT80.7662835249042145Clontech Universal Primer Mix Long (96% over 26bp)
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG50.4789272030651341Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50.4789272030651341Clontech Universal Primer Mix Long (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40.38314176245210724No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG40.38314176245210724Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT30.28735632183908044Clontech Universal Primer Mix Long (100% over 23bp)
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC30.28735632183908044Clontech SMART CDS Primer II A (96% over 26bp)
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACCTGTCTCTTAT30.28735632183908044Clontech Universal Primer Mix Long (96% over 26bp)
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC30.28735632183908044Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA30.28735632183908044Clontech Universal Primer Mix Long (96% over 26bp)
GATTACCATGGCAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTC30.28735632183908044No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA30.28735632183908044Clontech Universal Primer Mix Long (96% over 26bp)
GGCATTTAGGACATACTTTTTTTTTTTTAAGACAGAGTCTTGCTCTATCA30.28735632183908044No Hit
GTATAGTAGTTCGCTTTGACTGGTGAAGTCTTAGCATGTACTGCTCGGAG30.28735632183908044No Hit
GTCGAGCCCGATCTCCTCCTCGGCCTCGCGCAGCGCTGCATCCAGCGGCG30.28735632183908044No Hit
AAAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGA20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
GTGAAGGGTAGCTGCCTATTAGAATGGCTAAGAGAATGGAGCCAGACCAT20.19157088122605362No Hit
GCCACACGGGTCCGGTGCCCGCTGCGCTTCCGCCCCAGCGCTCCTGAGGC20.19157088122605362No Hit
CAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAA20.19157088122605362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGAGCGGCGTCCGCCAAGTTACTTCTACCGCCAGCACCTTCGTGAAGC20.19157088122605362No Hit
CTCGTATGCCGTCTCTGCTTGCCCATGTACTCTGCGTTGATACCTGTCTC20.19157088122605362No Hit
CCATTATTCAAAACACTTTCGGAGCTTCCCATGGAAACTGCCTCAGGCAG20.19157088122605362No Hit
GATTGCAGTTATTCTTGCGGTAGCTTTAAATACTTTCATTGTTTAGAGGT20.19157088122605362No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACTTT20.19157088122605362Clontech SMART CDS Primer II A (100% over 26bp)
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACCA20.19157088122605362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCCATGTGGAACTGTGAACGCTCCATTAAACCTCTTTCCTTTGTAAATTG20.19157088122605362No Hit
GATCCGATGCAGGGGATTAAAAAGAAAGCGAGAGTTCAGGGACTCTGGCG20.19157088122605362No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20.19157088122605362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCTCTGAGACAGTCCTTGGAGGGCAGAGGCAAGGCTCCCTAAGATTA20.19157088122605362No Hit
GTACATGAAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC20.19157088122605362Clontech Universal Primer Mix Long (96% over 25bp)
GCCCTAGCTGAAGTGCAATGCGGCGATCTTGGCTCACAGCAACCTCCACC20.19157088122605362No Hit
CTTCCTATCATTGTGAAGCAGAATTCACCAAGCGTTGGATTGTTCACCCA20.19157088122605362No Hit
ATACCATAAACCATGTGGCTTATGAACAACTGAATTTTCCACAGTTCAAG20.19157088122605362No Hit
GCGTTTGCTTCATGGAAAATTTTTTTCCTAGTCTTGCTAGTTCTTACTTT20.19157088122605362No Hit
GGTCATGGCCGACAAGGTATAGACAGCATCCTTGGTGTGGATCTGGAAGC20.19157088122605362No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
CTCTTCATAGTTTACTTGACCATCACCATCAATATCTGCTTCCCTGATCA20.19157088122605362No Hit
GTAAACGCTTCGGGCCCCGCGGGACACTCAGCTAAGAGCATCGAGGGGGC20.19157088122605362No Hit
GTGTACCGGTGCTATCTCTTTCATAGCGATCCGATGCTCAGCGGCTTCGT20.19157088122605362No Hit
CACCTAATTTTTTTCTTTTTTGTTCTTATTTTAAAAGGAAACATTTTCCT20.19157088122605362No Hit
GTGGTGGCGCGCGAGCACGGCAACATCCACCGCAGCGGCGAGCTCAACGG20.19157088122605362No Hit
CCTGAACCGTGAAGCCAACCAGGGCTGGGCGATCAATGCCCAGACCGAAC20.19157088122605362No Hit
ACACAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTAG20.19157088122605362Illumina Single End PCR Primer 1 (100% over 22bp)
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGACCCCTCTACTC20.19157088122605362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTCGCTCTGGGTGCCCACCCTGCTGACGGAGGAGACCGCTGTCTCTTA20.19157088122605362No Hit
TCCACTTGAAGTCTCACACCTCAGGAGAAAATGAGGAGGGAAAACTGGCT20.19157088122605362No Hit
CCATAAACGATGCCGACCGGCGATGCGGCGGCGTTATTCCCATGACCCGC20.19157088122605362No Hit
CTATTGCTCCTAGGCTGTAAACCTGTACAGATGTTACTGTAATGAATATT20.19157088122605362No Hit
GAAGCAGTGGTATCAACGCAGAGTACATGGGAGACGCCGAGACTACACTC20.19157088122605362Clontech SMARTer II A Oligonucleotide (100% over 25bp)
CATTTGAAGGAAGAGTTGATACAAAGGGAAGAAGGAATAAAAGTTTCTGC20.19157088122605362No Hit
ATATATTGTTCTAGGGTAAATGTTTTGTTTCCTTCAGCATTTTCAATATG20.19157088122605362No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC20.19157088122605362No Hit
GCCAAGGCGGGCGAATCGCAAGGTTAGGAGTTTGAGACCAGCCTGGCCAA20.19157088122605362No Hit
AGCACATACACCAAATGTCTGAACCTGCGGTTCCTCTCGTACTGAGCAGG20.19157088122605362No Hit
GTCGCTACCGGTATCGAAGTTGGGCAGTTCGCGCCCGATATCGGCCGCCA20.19157088122605362No Hit
GTACTGGGAACGCCGAAGGTGGGAGGCCATTTCATAACATTTCTTGTTGA20.19157088122605362No Hit
CCTACGGCCTCCCCAAATCTTCCACATTAGGTGGCAGAGGACAGTTGCAC20.19157088122605362No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAACG20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
CGTTAAAGGATTTAAAGTGGACTCATTCCAATTACAGGGCCTCGAAAGAG20.19157088122605362No Hit
ACTGTGGCTCAGGGCCTCCTGAGGAGGTGTACTCGGCGGGAGATTCTGTC20.19157088122605362No Hit
GCCATAGTTCACATTTGTGGTAGTTTTAGTTTCCCTCTTTGTGAGGTAAG20.19157088122605362No Hit
ACCATGCACTGCTGAATGACTGCCCAACGGCAGCCCACATGGGAACGAAT20.19157088122605362No Hit
GTATGCTCCTCCTAGAATAACACCTGCAAGCATAAATGCTAGTCCAAAGC20.19157088122605362No Hit
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCCTGTCTC20.19157088122605362No Hit
GTACATGGGGGAATTCGAGCTCGCATTTTGAAAATTCTATGGAAGAGCTA20.19157088122605362No Hit
CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT20.19157088122605362Clontech Universal Primer Mix Long (96% over 26bp)
GGAAGGGTCCTAATAAAAATCCCATGTGACTCAGAAAGCCCTAGCCTTGA20.19157088122605362No Hit
GCTTGTGGAGACCCAGCCTCGCTCTGGTTGTAGCGGCGGAGCAGGTTCCT20.19157088122605362No Hit
ATATAGCCGAGCGTACCCGCGTAGGTCAGCCGCGTGCCCGCGTCGGTGCG20.19157088122605362No Hit
CATACAGCAAACAGGAAATGAGAACATTTCAGCAAGATTTCAAGCAAGCA20.19157088122605362No Hit
ATTGATAGCACCCCAAATTCATGATTACCCACTTCAAATAGGCACTCAGT20.19157088122605362No Hit
GGAAGTTAAAGCCTGCCATCTTGTGGCGAGTTCATACAATTACACTAATA20.19157088122605362No Hit
GAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATAAACGCTTGG20.19157088122605362No Hit
GCCAATGTATGACCTGGAAGGAGGGAGATCTTAATCCAGTGTTGGCAACG20.19157088122605362No Hit
GAAGTAGGTGGTGATCGACACCGTGCCCGCCGGCACGTGCTTCGCGCGGC20.19157088122605362No Hit
CATTGTGAACTTTGGGGGATGCTCGCTCCAACCGACTGCTGTCACCTTCA20.19157088122605362No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20.19157088122605362No Hit
AAGTAAATGCAACTGATTATTATAAGAACCTCATTCATCTCCAATTTTTG20.19157088122605362No Hit
CTATTACATTCAATTCCTTTGGAGTCCGTTCCATAACAATCCATTCATTT20.19157088122605362No Hit
TTTGGGAAGATTGAAGTTTTTTTGAGAAAACAAACCATAAACTGGCTCTT20.19157088122605362No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)