FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003681752

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003681752
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length100
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAGCTCGCCGAGAATCTCGCCGACGCGCGGTGACTCGCCGAGGCCGA36.8181818181818175No Hit
ACCCACGCACGCAGCACGCGTGCGGCACGACCCGGTGAGACCTGGCGCAG36.8181818181818175No Hit
AGGCCAGCTTGCCCTGCTTGTGGAGCACGTCCGACAGGTAGGTCTGCTCG24.545454545454546No Hit
CTACTGGACCAAGTAAATAACGGTGAAGACGCCGATCCACACAACGTCAA24.545454545454546No Hit
GCTTTCCCAGATTGTCGATTTGCCTGCCGGCTCGATATCGGGTGTTGCTC24.545454545454546No Hit
CTCGAGGCGGCGGAGCTGCGCTGGGCGGCCTCACGGTCGGCGACGGCGCC12.272727272727273No Hit
GAAGGAGTTTTGCCTCATAGCCATCACAGCTGGTAATATGCTGAGTCTCC12.272727272727273No Hit
GAGCAGGAGAAGGAGATCAGGTATGGTGGGATGGTAGAAGGCATTTTTCA12.272727272727273No Hit
GTGCTGGGGGGCCAGCGAGCTGATGCTCGAGGGCAGAGCCCTCAGCGGCA12.272727272727273No Hit
CCTGCGACAAGTAACGATGCTGTCAATGCTGCTTTCCTTGCAATCTCGTT12.272727272727273No Hit
GCGCAGGATCTGTTGCTGTTCTACATTTTCTTCGAAGCCGGCCTGATCCC12.272727272727273No Hit
CCCCTCGCTCGAGCCGCCGCCGAAGAGGAGAGACGCCGCGAGGAGCACAC12.272727272727273No Hit
GCCAGATCCGCACCAGCGCGTCGCGGGAAGGCATCGGTGCATTCTGCCCT12.272727272727273No Hit
GTCAGGGCTGCAGCGGTGGCGACGACGTTGGTGCCCTTGCTGTCGTCGAA12.272727272727273No Hit
CAGATAGCCAAGGAAAGTGGTGTAGTGGACTTTTGTCATCTGAACACCCT12.272727272727273No Hit
ATGATAGGAAGAGCCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAA12.272727272727273No Hit
GAGTGGTCTCGGAATCAGCGGGGCTGTGCTCCAGTCCGTGAACCACGGGC12.272727272727273No Hit
TTCTCATATATTGAACGAAGTTTCAATGAAATCCGAACGAGGACCCGCAC12.272727272727273No Hit
GTTTGGCTTTTGTTGTCTATAGCAATAACCTACATCTAAGTCACATCCTG12.272727272727273No Hit
CGGTTGGGGTGCTTCCTGTCGGCCTTGTCCTGGCTGGAGTCGGACTCGGG12.272727272727273No Hit
TCACCCAGCAGCTCGTGCGCGGGCTGCGGCTGGCCGAAGGCAAGGACCAT12.272727272727273No Hit
GGCTACCCCAATGAGAACAAGGAGCCCTCCCACATCCGAGTCCGGGATTG12.272727272727273No Hit
CCTTGTTAAGAACAGAATGGGCCGGGCGCGGTGGCTCAAGCCTGTAATCC12.272727272727273No Hit
TAGCACTGTTGGCTGACTACTCCACAATTTCTCCCCATTCCTCCAAACTG12.272727272727273No Hit
GTCAGGGCTGCAGCGGTGGCGCCGACGTTGGTGCCCTTGCTGTCGTCGAA12.272727272727273No Hit
CCTCGGGGCCCCCCCTCAGACACCATGTGGCTTGAGGACACCTCCTTCTC12.272727272727273No Hit
CCTTTATGACGTATGCACTCACCTAACAGAGAAGAACCTTCCTTTTGCCA12.272727272727273No Hit
CCGGTAAACGTGCGATCGTGCTGACAAGCCGTGGCGGTATTCACAAAGAT12.272727272727273No Hit
CATAAAAAAACAAATTAAATTGCAACCAACAGAAAAAAATAAAAAACTAC12.272727272727273No Hit
ATCCTGCATGGTTTGAGAGGAAACAGCAAAGTCTTCACTCCAACCAATAA12.272727272727273No Hit
GGTGAGCATTCGACAGCCTGGAGCAGCACCCACAACCCAAGGCGTGCATC12.272727272727273No Hit
ACGCCAACACCTTTCCCGACGGGCGCAAGGTCAAGAGCACTTCCATCGGC12.272727272727273No Hit
CTTCCCGGGATTTACGTGCTCCACTCCCTCTCATTTCTTTGAACATGCCT12.272727272727273No Hit
CTCCCCAATCACTCCTTCTCACCTCCCCCAAAGTCCTAAGAATAATTTTC12.272727272727273No Hit
CACAACCCCCTGAAGACCAGGGCTTTTCTTTATATTTGCTGTTCCCTCCG12.272727272727273No Hit
GCCCTGAACGCCCTCGCCGCCGTGGTGCGCGAAGGCAGCTTCGAACGCGC12.272727272727273No Hit
GTTCAGGGCTGCGAAGTGAAGGCCTGCAAGGTCATCACAGGCCCGATCGA12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)