FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003681753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003681753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44
Sequences flagged as poor quality0
Sequence length100
%GC61

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTCGAGGCGCTCGCGCGCAGGCTGCCTCCAGCCGAGATCGACGAGTGG36.8181818181818175No Hit
CTTCCGGCGTCGATGGCCTGGCGGCGATGTCCACGCACAGCCGCCTTGGC36.8181818181818175No Hit
TTCCTCGACCTCGACGTGGTCGTCGTGGGCAGCCTGGACGCCTTCTTCGA24.545454545454546No Hit
ACCGAGCACCTTAATGCACATGACGTGACGCGCACCAGTGTTATCGGCCA24.545454545454546No Hit
GCGTTGTGTTCCTGTGCATCATGGCATTCGAGTGGACCGAGTTGATTCAC24.545454545454546No Hit
TGGCCATGCTGCCCGCGCCCGTCAAGGTCAAGCTGCAGCGTCCGCAGGTG24.545454545454546No Hit
GCCTCTGAGGTCTGGCTCCCCAAACAAGTCAGGCAAGCTTACACATTAGG12.272727272727273No Hit
GCTTGCGGTCGGCGGGCTTCAGCGAGCGCGGCGTGGTCGCGCGTGTGGCC12.272727272727273No Hit
GTGCCGTTATTGCAGCAGCGTTGGCCACATTTTGCCGAAGCAACGGCTCG12.272727272727273No Hit
GCACCACAGGGACCATTTGCTGGAGATGCAGCACTTGCCACAGCCACCAC12.272727272727273No Hit
CTCCCTCTGGGGGCATCTCTGCCGCGGCCGGGAGCCAGAGGCCAAGATGC12.272727272727273No Hit
TTGTTATACCTGGAGATCTTGAATGAAAGCCGTAGTCACGCGTGTTCGTT12.272727272727273No Hit
GGTTATTGGTTGGAGTGAAGACTTTGCTGTTTCCTCTCAAACCATGCAGG12.272727272727273No Hit
CTCCGCAGTTCCGCGTCACTCGGGGGTCCGCCGGTTGTCACGTTCTGACG12.272727272727273No Hit
GCTTGGAGGCGGCGAGAAACTCGCTGGGCGTGCGGAAATCGTGCGAGGTG12.272727272727273No Hit
TTCGTGTGGAGGACCGCCGAGATGAGCCGGAGCATGTACATCGCCGCGAG12.272727272727273No Hit
GCGCCAGGCTCCTGTGCAGTGAAGGCTGCCGCGGCGGCGACGAACCAGGT12.272727272727273No Hit
TGTGGGTGCTGCTCCAGGCTGTCGGATGCTCGCCGGGCGGTGTGGGTGCT12.272727272727273No Hit
CATCACAGGCTGGGCTTTCTGGAAGCAGCTTTGAGACAAAGGTTGGGGTG12.272727272727273No Hit
GGCTCGCCACCTCCGAAGTGGCACATCAACTACATGAAGGCAGATCGCAG12.272727272727273No Hit
GTCGACGTAGACCGAGTGCTCCACCGTGTACTTGTAGGCGGGAAAGGCGC12.272727272727273No Hit
GACACATCCTTGGTTCGTGTTGATACATTCCAGACACTGAGGCTACAACT12.272727272727273No Hit
GTGTGGGAGAACGCGGTGTCGGAGCGGCTGCGAAGCCCCGTGTTCTCGCG12.272727272727273No Hit
GGAGATGGAGCGCAGAATGGCGCCGCGGGAGATCCCGTCGAAGTTCACAG12.272727272727273No Hit
ATCTCTTACAGTATGTCAGAGGAAAGCATGAAGGAGTCTATCGTAAACAT12.272727272727273No Hit
TCCCCAGCAACTCCAGTCCTTGCATCATGAGATCGGAAGAGCGTCGTGTA12.272727272727273Illumina Single End PCR Primer 1 (100% over 21bp)
GAATGCAGACATCACAGAGCTGTTTCTGAGAATGCTTCTGTCTAGATTTT12.272727272727273No Hit
CGTGAGCACCGAGGACTCTGCCGCGCAGCGCCCGGCCGCCTCGAACGGGT12.272727272727273No Hit
GTTGGGGTATCTTTGTGAATACCGCCACGGCTTGTCAGCACGATCGCACG12.272727272727273No Hit
CAGTAGCGCGGTCTCGGCTCACTGCTAGCTCCGCCTCCCGGGTTCACGCC12.272727272727273No Hit
GACGTGGCTCTGGCTCGCCTTCTTCGCCAGCCTTGCGGTCAAGATGCCGA12.272727272727273No Hit
GGTTCACGCCATTCTCCTGCCTCAGCCTCCCAAGTAGCTGGGACTACAGG12.272727272727273No Hit
CCAAGCGCCCGGCCGCGCGCCGGCCGGGCGCGACCCGCTCCGGGGACAGT12.272727272727273No Hit
AGGTGTAGACGGCGAACGCTGCGAGGACGAAGATCACGGGCCCTGTCGCG12.272727272727273No Hit
TATCAACGCAGAGTACATGGGTGATGTAGAAGAGAGTCCCGCGGGAGGGT12.272727272727273No Hit
ACTCTTAGGCGTGTCCTAGTGCCGAACTGGGCCCAGAGACAGTGGACTGG12.272727272727273No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)