FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003682014

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682014
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6835125
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119560.17491999049029827No Hit
GTACGAGCAGTGGTATCAACGCAGAGTACGAGCAGTGGTATCAACGCAGA118780.17377882628335253Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACAAGCAGTGGTATCAACGCAGAGTACGGGCAGTGGTATCAACGCAGA102420.14984363855818292Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACGGGCAGTGGTATCAACGCAGAGTACGAGCAGTGGTATCAACGCAGA102180.14949251110989192Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GAGTACGAGCAGTGGTATCAACGCAGAGTACGAGCAGTGGTATCAACGCA96790.14160677383368994Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACGGGCAGTGGTATCAACGCAGAGTACGAGCAGTGGTATCAACGCA88010.12876136135037763Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACAAGCAGTGGTATCAACGCAGAGTACGAGCAGTGGTATCAACGCAGA81660.11947111428101168Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)