FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003682015

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682015
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6835125
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108400.1585925641447669No Hit
GTACGAGCAGTGGTATCAACGCAGAGTACGAGCAGTGGTATCAACGCAGA95980.14042171869570785Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACGGGCAGTGGTATCAACGCAGAGTACGAGCAGTGGTATCAACGCAGA80670.11802271355681132Clontech SMARTer II A Oligonucleotide (100% over 23bp)
GTACAAGCAGTGGTATCAACGCAGAGTACGAGCAGTGGTATCAACGCAGA68670.10046634114226148Clontech SMARTer II A Oligonucleotide (100% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)