FastQCFastQC Report
Mon 5 Sep 2022
EGAF00003682038

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682038
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6762556
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC383730.5674333787402278Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC383590.5672263564249967Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA220820.32653334035237563Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA188800.2791843793973758Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG141150.20872285567764612Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA121700.17996154116875335Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120310.1779061053246731No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN104640.15473439332702013No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC103610.15321130057924845Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG101490.15007639123432026Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA76420.11300460949972171Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTAT70360.10404350071186101Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)