FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003682046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4177769
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGAAGCAGTGGTATCAACGCAGAGTACGAAGCAGTGGTATCAACGCA112090.2683010956326211Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCGTACTCTGCGTTGATACCACTGCTTCGTACTCTGCGTTGATACCA69760.16697907423794853Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63400.15175563799721814No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC62410.1493859521672931Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGAAGCAGTGGTATCAACGCAGAGTACGAAGCAGTGGTATCAACG48240.11546832771270983Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC45800.10962788990966231Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)