FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003682052

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682052
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3701773
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN249150.6730558572878456No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGCAGTGGTATCAA86080.232537219327063Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCGTACTCTGCGTTGATACCACTGCTCCCGTACTCTGCGTTGATACCAC82390.22256902300600281Clontech SMART CDS Primer II A (96% over 25bp)
GCTCCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATAC76450.20652265819649124Clontech SMART CDS Primer II A (96% over 26bp)
CCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCAC66120.17861711131395686Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA64040.17299818222241073Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA55810.15076559259576425Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCAC51090.13801494581110188Clontech SMART CDS Primer II A (96% over 26bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT49950.13493534044361985No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTCCCGTACTCTGCGTTGATAC46930.1267770876280096Clontech SMART CDS Primer II A (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39470.10662458232852204No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)