FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003682053

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682053
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3701773
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN245670.6636549566923742No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG160210.43279261045990663No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGCAGTGGTATCAA79090.21365437588960748Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA64930.17540243553562038Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA55270.1493068321585359Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATAC47530.1283979325582633Clontech SMART CDS Primer II A (96% over 26bp)
TCCGTACTCTGCGTTGATACCACTGCTCCCGTACTCTGCGTTGATACCAC44870.12121218670080526Clontech SMART CDS Primer II A (96% over 25bp)
GTATCAACGCAGAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGC41500.11210844100921369Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCAC39280.10611131476727503Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)