FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003682074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3931336
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN180390.4588516473789063No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA62410.15875010429024639Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCAC52330.1331099656707033Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41050.10441742959645271No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)