FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003682126

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682126
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5476445
Sequences flagged as poor quality0
Sequence length35-76
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC416930.7613150501831024Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC325040.5935237183976101Clontech Universal Primer Mix Long (96% over 26bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN322350.5886117727832563No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA190630.3480907778677591Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA132390.24174441631386784Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG116710.2131127035878202Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA114530.20913201903789777Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTAT96960.1770491623671926Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA93070.16994601424829428Clontech Universal Primer Mix Long (96% over 26bp)
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC91230.16658617040799278Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG90670.16556360923920538Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA90170.16465060819564517Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC89720.163828907256441Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC86140.15729181978455Clontech Universal Primer Mix Long (96% over 26bp)
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC85890.15683531926276992Clontech Universal Primer Mix Long (96% over 26bp)
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG79990.14606190694875965Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA65970.12046135768733185Clontech Universal Primer Mix Long (96% over 26bp)
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA65720.12000485716555175Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC60460.1104000861872985Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG60440.1103635661455561Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA59730.10906710466370065Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG59150.1080080234531708Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC58950.10764282303574672Clontech Universal Primer Mix Long (96% over 26bp)
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC58770.10731414266006506Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGGTGATAC56400.10298651771358976Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG55050.10052141489597721Clontech Universal Primer Mix Long (96% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG54970.10037533472900759Clontech Universal Primer Mix Long (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)