FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003682127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5476445
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN313660.5727438146461802No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC282590.5160099297993498Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC180760.3300681372678809Clontech Universal Primer Mix Long (96% over 26bp)
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA148630.2713986902087029Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA115650.21117714137547258Clontech Universal Primer Mix Long (96% over 26bp)
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAG99800.18223500829461448Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA86900.1586795813707615Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG85220.15561189786439927Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC84270.1538771958816349Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA80810.14755922866019835Clontech Universal Primer Mix Long (96% over 26bp)
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC77290.14113170131353459Clontech Universal Primer Mix Long (96% over 26bp)
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA76540.13976219974819432Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG76220.1391778790803158No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTAC74290.13565369505217345Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAA61560.11240868848313094Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACAATTCTATTATAC58990.10771586311923154Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG56270.1027491374422641Clontech Universal Primer Mix Long (96% over 26bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC56190.10260305727529448Clontech Universal Primer Mix Long (96% over 26bp)
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG56150.10253001719180965Clontech Universal Primer Mix Long (96% over 26bp)
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCA56150.10253001719180965Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)