FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003682197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8308534
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148860.17916518124617412No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGTTAAGCAGTGGTATC106990.12877121282767814Clontech Universal Primer Mix Long (96% over 26bp)
ACGTTAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG98810.11892591400600877Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TTCCCGTACTCTGCGTTGATACCACTGCTTAACGTACTCTGCGTTGATAC98180.1181676574952934Clontech SMART CDS Primer II A (96% over 26bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98150.1181315500424022No Hit
GCTTAACGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGAT91930.11064527147629172Clontech Universal Primer Mix Long (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)