FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003682200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4046379
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN186420.46070820355680964No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT156400.38651841560071365No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT88840.21955432251897314TruSeq Adapter, Index 10 (95% over 21bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG87270.21567431029075626No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC69070.17069582458785992No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC63250.15631259454440624No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA57800.14284376228722023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57020.1409161128999533No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT51910.1282875380679862RNA PCR Primer, Index 10 (95% over 23bp)
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC51510.12729899992066981No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA49320.1218867535641125No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC41970.10372236510717359No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)