FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003682202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8270692
Sequences flagged as poor quality0
Sequence length35-76
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTCCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATAC559490.676473020637209Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA521420.6304430149254742Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATAC450920.5452022636074466Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGCAGTGGTATCAA355950.4303751125057009Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCAC302990.3663417764801301Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA297550.35976433410892344Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTTCCGTACTCTGCGTTGATACCACTGCTCCCGTACTCTGCGTTGATAC281330.3401529158624212Clontech SMART CDS Primer II A (96% over 25bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG212220.25659279779733063Clontech Universal Primer Mix Long (96% over 25bp)
CCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCAC192390.2326165694478769Clontech SMART CDS Primer II A (96% over 26bp)
TCCGTACTCTGCGTTGATACCACTGCTCCCGTACTCTGCGTTGATACCAC183720.22213377066876627Clontech SMART CDS Primer II A (96% over 25bp)
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGC152650.18456738565527528Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGCAGTGGTATCAACG146510.17714358121424423Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGC138930.1679786890867173Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA138280.16719278145045177Clontech Universal Primer Mix Long (96% over 25bp)
GCTTCCGTACTCTGCGTTGATACCACTGCTACCGTACTCTGCGTTGATAC135570.16391615115156022Clontech SMART CDS Primer II A (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN132660.16039770311843313No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTA127530.15419507823529155Clontech SMART CDS Primer II A (96% over 26bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA124070.15001163143301674Clontech Universal Primer Mix Long (96% over 25bp)
GTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG120400.14557427600979458Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTCCCGTACTCTGCGTTGATACCACTGCTCCCGTACTCTGCGTTGATAC115030.13908146984557035Clontech SMART CDS Primer II A (96% over 25bp)
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT114640.13860992526381105Clontech Universal Primer Mix Long (96% over 25bp)
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGC112490.13601038462077902Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCGTTGATACCACTGCTCCCGTACTCTGCGTTGATACCACTGCTTCCGTA110250.13330202599734095Clontech SMART CDS Primer II A (96% over 26bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGCAGTGGTATCAA103650.12532204076756817Clontech Universal Primer Mix Long (96% over 25bp)
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG98090.11859950775582019Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTCCCGTA92490.11182861119722509Clontech SMART CDS Primer II A (96% over 25bp)
ACGCAGAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT91300.11038979567852363Clontech SMARTer II A Oligonucleotide (96% over 25bp)
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGCAGTGGT88670.1072098924733263Clontech Universal Primer Mix Long (96% over 25bp)
CTGCTCCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGAT82760.10006417842666612Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)