FastQCFastQC Report
Tue 13 Sep 2022
EGAF00003682203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8270692
Sequences flagged as poor quality0
Sequence length35-76
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA551330.666606857080399Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTCCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATAC453330.5481161673049848Clontech SMART CDS Primer II A (96% over 26bp)
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATAC338550.409336969627209Clontech SMART CDS Primer II A (96% over 26bp)
GAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA307300.37155294865290595Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGCAGTGGTATCAA297860.3601391515969885Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCAC231400.27978311851051885Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGC213970.2587087029718916Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATAAA206140.24924153867657023Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GCTTCCGTACTCTGCGTTGATACCACTGCTCCCGTACTCTGCGTTGATAC205000.24786317759142767Clontech SMART CDS Primer II A (96% over 25bp)
GCTTCCGTACTCTGCGTTGATACCACTGCTACCGTACTCTGCGTTGATAC168650.20391280439411844Clontech SMART CDS Primer II A (96% over 25bp)
GTATCAACGCAGAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGC166880.2017727174461339Clontech SMARTer II A Oligonucleotide (96% over 25bp)
CCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCAC157480.19040728393706355Clontech SMART CDS Primer II A (96% over 26bp)
TCCGTACTCTGCGTTGATACCACTGCTCCCGTACTCTGCGTTGATACCAC137890.1667212368686925Clontech SMART CDS Primer II A (96% over 25bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACG129630.15673416444476473Clontech Universal Primer Mix Long (96% over 25bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA125460.15169226468595373Clontech Universal Primer Mix Long (96% over 25bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN120220.1453566400489826No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG117840.14247900901157967Clontech Universal Primer Mix Long (96% over 25bp)
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT117310.1418381920158555Clontech Universal Primer Mix Long (96% over 25bp)
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTA110240.1332899351106292Clontech SMART CDS Primer II A (96% over 26bp)
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGA102310.12370186194819008Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA102080.1234237715538192Clontech Universal Primer Mix Long (96% over 25bp)
GAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATAAA100570.12159804766034088Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCTCCCGTACTCTGCGTTGATACCACTGCTACCGTACTCTGCGTTGATAC96780.11701560159657742Clontech SMART CDS Primer II A (96% over 25bp)
GCGTTGATACCACTGCTCCCGTACTCTGCGTTGATACCACTGCTTCCGTA92900.11232433755240794Clontech SMART CDS Primer II A (96% over 26bp)
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGGAGC90270.1091444343472106Clontech SMARTer II A Oligonucleotide (100% over 25bp)
ACGCAGAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT89010.10762098262152671Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GGTATCAACGCAGAGTACGGGAGCAGTGGTATCAACGCAGAGTACGGAAG88690.10723407424674984Clontech SMARTer II A Oligonucleotide (96% over 25bp)
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTACCGTA85560.10344962670596365Clontech SMART CDS Primer II A (96% over 25bp)
GCTCCCGTACTCTGCGTTGATACCACTGCTCCCGTACTCTGCGTTGATAC85390.10324408163186345Clontech SMART CDS Primer II A (96% over 25bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)