FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003682304

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003682304
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5231402
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT146380.27981026883424365No Hit
GTACGAAGCAGTGGTATCAACGCAGAGTACGAAGCAGTGGTATCAACGCA107620.2057192316705923Clontech SMARTer II A Oligonucleotide (100% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65840.12585536343794645No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60090.11486404600525825No Hit
GCTTCGTACTCTGCGTTGATACCACTGCTTCGTACTCTGCGTTGATACCA53330.10194207977135002Clontech SMART CDS Primer II A (96% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)