FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003683020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003683020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3555412
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135120.3800403441288942No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63430.17840407806465186No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62540.17590085199689937No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN54150.15230302423460348No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA46830.1317146929807291Clontech Universal Primer Mix Long (96% over 26bp)
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC45580.12819892603163852Clontech Universal Primer Mix Long (96% over 26bp)
GTCCTAAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTTT37270.10482610735408442No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)