FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003683091

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003683091
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131
Sequences flagged as poor quality0
Sequence length50-76
%GC41

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCAATGGAAGTCATGGGTATGACACGGTGTGATTCACAAGAAGTGCCA10.7633587786259541No Hit
TATCAACGCAGAGTACGGGAGTCTTCTAAATTATGTTTGATAGTTGAAGT10.7633587786259541No Hit
GATTCACCGCCTAGTCCTCATATATTAACTTTTATTTATTTTACTCCAAATAAACATTCAGAATAAAAAAAAACA10.7633587786259541No Hit
GTTGCACGCAACCACGCCTGCCTAAATTTTTGTATTTTGTAGAGATGATG10.7633587786259541No Hit
CTTTTAACCAGTGAAATTGACCTGCCAGTGAAGAGGAGGGCATAACACAG10.7633587786259541No Hit
TTACAAAATATACAAATAAATAAAACAATTCAACTTTTAAAAGTATTACCATATGCAAGAAGAAGGTATTTCTAT10.7633587786259541No Hit
TTCAATTTAGAGTTTTTTTGTTTTTTGAGATGAAATTTCACTCTGTCGTCCAGACTGGAGTGCAG10.7633587786259541No Hit
GCAAAAAGATACACCATGTTATAAGTACTTACAAAGTTACAACCATTTGC10.7633587786259541No Hit
ATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAA10.7633587786259541No Hit
GATAAACCCCCTGAAGGATGTGATCATCGCAGACTGCGGCAAGATCGAGG10.7633587786259541No Hit
GGCAAATATGAGCATACAATCCCTTTGTTCTAAAGATATTGTTCCAGCTA10.7633587786259541No Hit
CTTTATTGATGGTACATGACAAGGTGCGGCTCCCTAGGCCCCTCCCCTCT10.7633587786259541No Hit
TTTAAAACTAAATATAAATTACATTTACAAACAAAAAGATAAATGAAAAA10.7633587786259541No Hit
ATCTTAGCTCACTGCAACCTCCGCCTCGCAGGTTCAATCCAATCTCCTGC10.7633587786259541No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAAAAAAAAAAATTAAA10.7633587786259541No Hit
TTCCATTGCTGTCTGGAATCAATTTATTGACCACTTCTTTCAAGTCATTTGTCTGCACCTCTCGGGTCATGATTT10.7633587786259541No Hit
CTTTCATCCGTATTATTTCACTTGATTTGGCTGCGCAATAGAATATTTAT10.7633587786259541No Hit
GGGCATAGGATACCTGCATCAGCTGTAACTTCTGCTTTTCTCCTTTGCCT10.7633587786259541No Hit
GAAGAAAACATCTGATCCACAGTGGGCATTTTACAACAATGGAACAGATA10.7633587786259541No Hit
CCCCCATGCCATCCTGCGTCTGGACCTGGCTGGCCGGGACCTGAGTGACTAGCTCAGGAAGCTCCTCACCGAGCG10.7633587786259541No Hit
ACTAAAAAATGTATAAAGCATATTCATGAATGGATTCAGTGAAGTAACAA10.7633587786259541No Hit
GGTCTGCCTGCATTTCTTCAATCATGAATTCTGAGTCCTTTGCTTCTATA10.7633587786259541No Hit
GGGCCTGTGCTTTAACGGCAGTGCCTTCGTCTTGTACCTCTCTGCCGCTGTTGTAGATGCATCTTCCGTCTCCC10.7633587786259541No Hit
GCTCAGCAGCTGTGGCAAGGGCAAGTAGTGAAAGCCAATAAGTTCAGTAATGTCACCCTGTCTATTATAAACAA10.7633587786259541No Hit
TCAGTGTGCTGGTTATAGAGGCTGTCTGGGCAATTCTTCCGGATGCTTTC10.7633587786259541No Hit
GAAAAGAATAGAGAAGATAGTGAAATTAGAAAATAAAAACATACTTTTAG10.7633587786259541No Hit
ACTTAGTCTCCGCACTCTAAACTAGATTTCTAATTCTGCACACTGAGATTTTTTCCCATAGGCTACCACCTCTG10.7633587786259541No Hit
ACTGGGGCCCTTTCTCCACGCGCTGCCTCCAGCCCCGGGCCCACTCAGTG10.7633587786259541No Hit
GGCGATGAGCCTCCTGTTCATCATCCTGCTTCGCTTGCTGGCTGGTATTT10.7633587786259541No Hit
CACTAAGTGACGTTTTTGAAAAATTATTGAACTGCTTGGAGTTTTGGTGT10.7633587786259541No Hit
TAAAGGAAGCGAGAATGCAGAGTTTCCTCTCTGATAACAAGCCCTTCAGA10.7633587786259541No Hit
GGTCAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7633587786259541No Hit
AAATTACTATGCATAAATACCACAGCTTAAAAACACCAAAGAAACAACAA10.7633587786259541No Hit
AAACAAAGAAGAGTACCGGCACAACCAAAAAACTCCAAAAAATATAACAG10.7633587786259541No Hit
ACATTATGTAACTGGGCTGTATGAGGTTGTTTAGAAGGCTAGGGTTAAAA10.7633587786259541No Hit
GTCTTTAATCCATCTTGAATTGATTTTTGTGTATGGTGTAAGGAAGGGATCCAGTTTCAGCTTTCTACATATGG10.7633587786259541No Hit
ATCTTGCACTGCTTCTTGGGAGTTTAAAGTGGATTATACTTGCAACATTA10.7633587786259541No Hit
GTCGATCCGCTTCACAGTCCGCTTGTAGTTCCCGACCTCCCAGAAGCTGT10.7633587786259541No Hit
ATATAGTGCTGAAAACACAACTCAATATAATGATTTTAAAAAATAAAGAAAACACAACATGTTCCTGGTAGG10.7633587786259541No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTAAATAATAATAAATAAAAA10.7633587786259541No Hit
CAATTAAGCTTGTCGTCTTTACCAACCATGCGAAGTAAACATGTTACATC10.7633587786259541No Hit
ACTTAGAAGCATTCAGAATGTCAACAAAACCGCTGCAACTTTTTTTTTTT10.7633587786259541No Hit
GTGCAGATCTGGAGTCCAGCCTCAGGGATGCGATACTTTCCATTCTCTGCATTGAACATTCGTTCTGTCAGCATC10.7633587786259541No Hit
TTTCAGTCCCTTCCAACTACCCCCAAAAAAGAAGGTAGTGAAAGGAAGGG10.7633587786259541No Hit
GAATTTGTTAGGAGAATACCTTTAATACAGTGACCACTTGATAGTAATAC10.7633587786259541No Hit
GAATCTTAGTATCCACTGCTTCTCGCAGGTTATAATTCACCATTGCACTA10.7633587786259541No Hit
AAGCTGTACATTCTTATAATCTACATGCTTTCCACACAAGATACATTTCTTAAGAGGTTCTTTATAAGGATTTTC10.7633587786259541No Hit
TTGCTGAGGCTTGTGAGGTTCCAACCGAATGTGACCCAAGAAAGTGCCGT10.7633587786259541No Hit
GAATGAGCCTTTCCGGACTGACGATCTAAATGTAACTGTTCGTGTGCCTG10.7633587786259541No Hit
ACTATGGACATCATAAGCTCAAGGAGAAGAAGTAGATTCGACAGCAGTGT10.7633587786259541No Hit
GAATAATCTTGTCCATGTAGTCTTCGACTGTACTTCGTGGTGATGCTCAA10.7633587786259541No Hit
CTACACCAATCTACCAACAAATGAAACAATACATTAAAAAACAAAACAAA10.7633587786259541No Hit
GTTTGTTAGGTACTGTTTGCATTAATAAATTAAAGCTCCATAGGGTCTTCTCGTCTTGCTGTGTTATGCCCGCCT10.7633587786259541No Hit
TCCTAATAGTGAGGAAGGGCTTCTAAAAGCTCACAGCTACAAAACAAAAA10.7633587786259541No Hit
ATTTGGAGTAGGGTCTTAATCACGTGAAAAGCAAAGCTGTTCACATTTAG10.7633587786259541No Hit
GGATACAAAATAGCAGATATGCAGAATGAACAAGTGTAGAGAGTTAATGT10.7633587786259541No Hit
GTAAAACCTTTGACCTGTTCACACCGTTTAGTGTGGGGCTTGTCTACCTG10.7633587786259541No Hit
GTCCAATTGTGTTTGTGCTGGGAATTGAGATTTTTGGTAGAGTTTAGGTA10.7633587786259541No Hit
CTTTAATAAGTGAGTGCCATAAGAAAGTCAATATATATTGTTTAAAAGAT10.7633587786259541No Hit
GAGTACAAGCAGAGCTATCAACGCAGAGTACAGGGGGTAGAATAATAGGA10.7633587786259541No Hit
TCTTAAAAAATAGTACTCCAATTTAAATAAAAATTAATTACACAATTGAA10.7633587786259541No Hit
GGGTGGAATAGCCCAGGCTTTGTTACAGTCTCCACTAGAAACAGGATGTC10.7633587786259541No Hit
ATGTTTTGTTTATTGGAACTTCTACAGCTTGATGCCTCCTGCTTATATAG10.7633587786259541No Hit
TTACCTCCCACTTCCGATGAACTGACCATTGCTGGCATGACGTTTACCCC10.7633587786259541No Hit
GGGCTGGGGCAGATGCAAGGGCCGGCGCAGCTGGACTCAGTGACCATAAACTCCTACGCCGCGCTCTCCCTGGCG10.7633587786259541No Hit
GGGTATGCCCTCCCCCATGCCATCCTCCGTCAGGACCTGGCTGGCCGGGACCTGACTGACTACCTCATGAAGATC10.7633587786259541No Hit
ACGAAGTACACACCTGACACTATCAACTAGCAATATGCGCAGTATTCTCA10.7633587786259541No Hit
ATCCGAAAGCCCCCAATGTCGTTGTCACCCGGCTCACCCTGGTTTGTGAG10.7633587786259541No Hit
CCCCAGGAAGATAAGGATTTCAAGAACAAACAAAAAGAGCACCAGAAAAA10.7633587786259541No Hit
GGCAGAAAGAAATGGAGACGTGGGAAGGGGACCATGTTGACTGGCAACTT10.7633587786259541No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGGACTATTCCTAGAGACACCATCAAGAACAAAGCTTAGATAAAC10.7633587786259541Clontech SMART CDS Primer II A (96% over 26bp)
ATATTAGGAAGCTTACATAATTTAATTTCCACAATGGAACAATAATGGCC10.7633587786259541No Hit
ACACTACCCAGTGTGTAGCCTTTTATCCCTTACCCACTCCCATTTTTCCT10.7633587786259541No Hit
CCCAACAATACTGTGTGGAAGATTGCAAGTGATTGCTTCCGTTTTCAAGA10.7633587786259541No Hit
GTATCAACGCAGAGTACGGGTCCTCCCTTACCCCTCCCTTCCCCAGCCCGACTTCTTGGCCTCCTGCCCAACCCG10.7633587786259541No Hit
ATGCTTTACAGAGATAAAAAAGCTAATTCTGCATTTGAACAGATCTTGGC10.7633587786259541No Hit
ACAGAGATCTTTGCACCAGTCTCTTCCTGCAGTCTTTTGATTGTATTCCC10.7633587786259541No Hit
ACGCTCTGATGGACACCTCGTGCCTGCACAGCCAGGCCCACGCTGGAGCG10.7633587786259541No Hit
GATCCAGGGTTAGGTTTTGTAGCCTCGGCTGGCCCGTCGGCCTCAGGCAC10.7633587786259541No Hit
TAAATATATGGCATGGCAAGAGTTTAAAAAAAAAGCAAATTCTCAAAGAA10.7633587786259541No Hit
CAAGTATAGCGACGACATAGGTAAGCGTCTGACGTCCGTGGTGCTGGGTG10.7633587786259541No Hit
CAACTCCACAACGTTTCGATAACTTTGATTCAATTCTTTAAGATCTGATT10.7633587786259541No Hit
GTGGTATTCAGCGGGTTCTTCACGGGGGTCGACGTGGGCCGGTAGAGCCG10.7633587786259541No Hit
TGTATAAACAGATCGAACAAGTTGTTTTTGATGATGAGTTTATTTTTAAT10.7633587786259541No Hit
CCACGTGTGTTATCGCCCTTGCAGCTGGGGGTGGCCACCACTGGGGAGAGGCAGGGTGAAGAGGTCGAAGGAGC10.7633587786259541No Hit
GTCTTAGTGATCCCCTGTCTGACAGAGTGCCTTCCTAATGACCTTGAGAT10.7633587786259541No Hit
GTTTCTTGAATGTTTCCCAAAATACATTGTAATACTTCCTGTGCATCATGCTGTAGATATCCTTCATACAT10.7633587786259541No Hit
AAATCATGACGCACATGAAGGATGACAACCCGACGAGAAAGCTCTCAAAA10.7633587786259541No Hit
AGACAGTTGCGTCCAGAGGCTTTTGGAAATCACCGAAGGGTCAGAATTCC10.7633587786259541No Hit
TTTAAAGGCTGCAGCGTCGTCCTCAAATTTCGCACTGACCAAATGTGATC10.7633587786259541No Hit
GTGAAGGAAAAAATAAAGACTTTCCCAGTTAAACAAAAATGGAGGAAATT10.7633587786259541No Hit
CTCTTTGGGAGTCCGAGGCAAGAGCTTGAGGCTAGGAGTTAAGAGACCAGCCTGGACAACATAGTGA10.7633587786259541No Hit
ACAGAAAGCTCAAAGGACAACAAAGCTGCACCTTCCTAAAGGCTGACCCT10.7633587786259541No Hit
GATCAAGATCATTGCTCCTCCTGAGCGCAAGTACTCCGTGTGGATCGGCGGCTCCATCCTGGCCTCGCTGTCCAC10.7633587786259541No Hit
ATATTAATTAATCAAACGTTTTTATTTTACTAAATAATCGATTATTTGAATAGATTAAAAAAAATTTTGGTTTT10.7633587786259541No Hit
GAGGGGGGGTGTGGTGGGCAGGTTTATTGGGAGCATGTTCGTCATCTAGG10.7633587786259541No Hit
CCTAGAAGCGGCGTGGGACTTCTAACACTTTCTTCGTTTTGGTCGAGGGA10.7633587786259541No Hit
CTAGCCTTCTGTCCCTCCCGCAGAAGACTGTTGGATTGAACAAAATAATA10.7633587786259541No Hit
ATCCCAGGGTAAACACGGCGCCGTTAGTCAAAGAGGAATGAGAAAGTGCT10.7633587786259541No Hit
GATCTAAGTGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCT10.7633587786259541No Hit
AAAATAAATAATATATAAATATAAAAAAAAAAAAAAAAAAAAAAAAAAAA10.7633587786259541No Hit
ATTACTATCCATCCTCATCCTAGCAATAATCCCCATCCTCCATATATCCA10.7633587786259541No Hit
CGGCACTGTGGGAGGTCTGTTGCTAGGTCCCATCACCGTGGTGCGCCGTA10.7633587786259541No Hit
GTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAA10.7633587786259541No Hit
AGTAAGTATGACGCTCGTGTCGCCTAAACAACGCAGAGCACTGGTAATTA10.7633587786259541No Hit
TTCCTGTTGATACCGTCTGCGATAGGCTAGTTCATAAACGAGGGGCGATG10.7633587786259541No Hit
GGGGTATACTACGGTCAATGCTCTGAAATCTGTGGAGCAAAACACAGTTTCATGCCCATCGTCCTAGAATTAATT10.7633587786259541No Hit
GGGCAATCATGACTTCATAGAGGCATACAAGTGCCTGACCGTGATAGTCAAATCGTTCTTGCGAGCATTCCACGC10.7633587786259541No Hit
ACAACTCAGAGTACGTTTTTTTTTTTTTTTTTTTATATTTTTTTTTTTTT10.7633587786259541No Hit
GTACGGGGAGATCTCTGCATGTCTGAACCATGACCTGGGCTACAGTCCTG10.7633587786259541No Hit
TGCCTATCCTCTTAGCCACAGTGTGTGCAGCCCCACAAGGGAAAGGAGCA10.7633587786259541No Hit
TCGGCATGCTCTTCATCATCAGCCTGAGGCTACTGATCGGCGTAGTCGAG10.7633587786259541No Hit
TCCACAGTCTTCTGGGTGGCAGTGATGGCATGGACTGTGGTCATGAGTCC10.7633587786259541No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTCATAACACAG10.7633587786259541Clontech SMART CDS Primer II A (96% over 26bp)
GGCTGGGTGAAATGGCATTTCTAGTTCTAGATCCTCAAGGAATCGCCACA10.7633587786259541No Hit
GGCTACTGGTGTCCTGGGGCTCTCGCGCGGGGTTGTCCGGGTAGCCTGCA10.7633587786259541No Hit
TCAAAAAACTTGGTATTTCAGGTGTAAATTACATGAGCTCTTTGCTTTGA10.7633587786259541No Hit
ATGTTGGGGGGAGGAAGGGGCCACCCACCAATCACCTATTATTGAAGCCG10.7633587786259541No Hit
ACCTTAAGGATACAGAAAGAATGTATTTGTGCCTTTCTCAAGAAAGAATAATTCAATATCAGGCCACTCCATGTC10.7633587786259541No Hit
AAACAGAGAGGGAAAAACATTTTAAAACATATAAACAACATTGCTACACA10.7633587786259541No Hit
GTCTACAATATCACCTTTCTTATAGATTAGCATATATGTGGCCAAAAGAA10.7633587786259541No Hit
GCCTCAGGCCATGGCCGAGATCGTCGAGGGGGGGTGTGGGGGTTGTCACT10.7633587786259541No Hit
TTTTGGATGAGTCTTTAGGGTTTCCTAGGTATATGATCATATCATCAGCA10.7633587786259541No Hit
GTTTCAAACCTGAGTACGGTTTTAGTTTTTTATTTTTTTTTTTTTTTTTT10.7633587786259541No Hit
CTCTTGTGGCTTGATCCAAGTTGTCAGCTACTAAGGTATCTCGTAAAGCA10.7633587786259541No Hit
GACTTAGGTCTGTTAGAGGAAGACGACGAGTTTGAAGAGTTCCCTGCCGA10.7633587786259541No Hit
AACTGTATAATATCCAACACCAAGGTCCAGTCAACAGCAAACCAAGCCTC10.7633587786259541No Hit
TTCTATGGCTGAAGGCTAGAGGGCTTAACATGAGGAATGATGACTCTATA10.7633587786259541No Hit
AAACAGTGAGGCTGAAAGGGGGGGCTATGGAAGAGAGGTAGGGAGTCAAA10.7633587786259541No Hit
GTCCGCGCTTATCTTCTCGGTGGCTCGCTTCCGAAGGAAGGCGTCCGCTTCATCCACACAGAGCAGGAGGCCGCG10.7633587786259541No Hit
GTTCTCCGCAGTGAGCAGCAGAGGGTGTCTGGTCTGCGCTGGAGCCGTGCAGGTGTGTCCCGGGGGAGTGGCTAC10.7633587786259541No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)