FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003683092

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003683092
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5914956
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG156790.26507382303435567No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149860.25335775955053597No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC124850.2110751119704018No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC117920.19935904848658215No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA100240.1694687162508056No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC98640.16676370880865385No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC90800.1535091723421104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86280.1458675263180318No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATC85120.14390639592247179Clontech Universal Primer Mix Long (96% over 26bp)
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC79640.13464174543310212No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC79580.13454030765402142No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT71460.12081239488510143No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70430.11907104634421625No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA67180.11357649997734556No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN63430.10723663878480244No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)