FastQCFastQC Report
Mon 12 Sep 2022
EGAF00003683093

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003683093
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5914956
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG144810.24482007981124457No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC115340.1949972239861125No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC108030.1826387212347818No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA95480.1614213191104042No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC90770.15345845345257006No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC88190.1490966289521004No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACAATTCTATTATAC75830.12820044646147832Clontech Universal Primer Mix Long (96% over 26bp)
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT75390.12745656941488662No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC72940.12331452676909177No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC72890.12322999528652454No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68990.1166365396462797No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA62250.10524169579621556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)