FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003683208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003683208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149
Sequences flagged as poor quality0
Sequence length35-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATACAACTTGGAATGGATTTTGAGGCAAATTGTGCCATAAGCAGATTT10.6711409395973155No Hit
CTCTAAATCCCTTGTAAATTTAACTGTTAGTCCAAAGAGGAACAGCTCTT10.6711409395973155No Hit
CATATACACCACTTACAGTAAGTAATAACTCAGAGTATAAAGTCGAAAAG10.6711409395973155No Hit
GGCACACCCCACTCATTCCAAGTGGGATTTTAGCTGTTCGCTCCAACTTT10.6711409395973155No Hit
GGGTTGTACAGGGTTTAGCACCATACTCTCGACCTCCACCCCACGTGCGCCTCCTGACTCGCCATCCAAAACAC10.6711409395973155No Hit
CAGTCTTGGCAGTGCAGATGAAAAACTGGGAACCATTTGTGTTGGGTCCA10.6711409395973155No Hit
GTCATTTTGCAAATGCCGCATGCAGGCAGCAGGAAGCCTGAGGCCTATGA10.6711409395973155No Hit
AGTTTCATGTTGGGTTTAGAAACGCATGACCGAAAATCAGAAGACAAACT10.6711409395973155No Hit
TCCCTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTTTTCCACTTCAAACTTCTATCTT10.6711409395973155No Hit
CATCCTTATTATACTTCTCAAATACTAGAAAGTAGTGCCATCTTTCATCATCCACGTCAATAAAGCTAGCAGTTT10.6711409395973155No Hit
TTGCACTGCTGAGAGCAAGATGGGTCACCAGCAGCTGTACTGGAGCCACCCGCGAAAATTCGTCCAGGGTTCTCG10.6711409395973155No Hit
AGATGGGGTTTCACCATGTTGGCCAGGCTGGTCTCTCACTCCTGACCTCAGGGTGTTCCACCCCCTACGGCCTCG10.6711409395973155No Hit
CATTAGGGCTTGTGGGGCAGAGCCAAGGGAGGGAGATCATCAGGAAAAATGGCTAATGCATGCTGAGCTTAATA10.6711409395973155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10.6711409395973155No Hit
ATTTTCTAGTTTATTTGCATAGAGGTGTTGATAGTATTCTCTGATGGTAG10.6711409395973155No Hit
GTAGATGGCTGGTTATGGGCTCCCCAAAATCCCTAAAGATCTCACTTAGT10.6711409395973155No Hit
GTAATTATATCAGGAATAGACAATTTCCTACACTGTCAAATATATAAAAGTTCCTTTTGCACTTTCTTCAACA10.6711409395973155No Hit
AGTATAAGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAG10.6711409395973155No Hit
GGGAAAGAGTGAGTGTCAAGAGGGGCCCCACAGAGATTTTGGAGTGCTGG10.6711409395973155No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG10.6711409395973155No Hit
CTTGTCTCATTTTCGGATAAGGAAGCTGAGGCTCCAAGAGGTCCAGCAGC10.6711409395973155No Hit
GGCAATATCAAATTTCCTATGGAATCTACTGCTGCTATTTAGGGTAAAATAAATTTTCTCAAAACGGACTTTGA10.6711409395973155No Hit
TGGCCTCACTTTCACTTCATAATCATATTTTGCTGTATACGTATGGATCA10.6711409395973155No Hit
GCCTCACACTCACCACAAAACTAACTAATACTAACATCGCAGCCGCTCAG10.6711409395973155No Hit
GTCAGGAACCCTGTGTGATCATGGTCTGGATCTGCAACACGGGCCAGGCC10.6711409395973155No Hit
GAATAATCAGTTTCTGTACAAAGATTTACAGATGTCCAAGTATGTAGTTT10.6711409395973155No Hit
GAGAAGAGAAATGTATAATTTTATCCCATTTTTAATATTTTGGTCTAGCA10.6711409395973155No Hit
ACTCCATACATTTCTAGATCTTGTGCAATCTTCAGGTATTCCATCATACA10.6711409395973155No Hit
CACTTGTAATAAACTGAAAAAATATTTCAATTAAAACAACCATATGCCAC10.6711409395973155No Hit
CAGTGGACCGTGGGCAGCCAGGGTCGGTGAAGAATCCCAACATGGCTGGG10.6711409395973155No Hit
TGAATTAACTAGAAATAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCG10.6711409395973155No Hit
GTCCCATATTAATCAGAGATGGCAAAAGAAAAAGTTGTCATATTACCAGG10.6711409395973155No Hit
GCTCTACGTTACAGGTGGGGCATGTCCTCATCCTTTCCTGCCATAAAAGC10.6711409395973155No Hit
GGATTTTTCTCTTGCGTCTCTGTCTTCTTCAGTTTATACTTATAGAATAT10.6711409395973155No Hit
CATCAGTCTTTATCCTGAGTACACAAAGGATCTATGAAATGTGGGTTTTG10.6711409395973155No Hit
CCTCAAAATCTTTCATGACATCGTAGTTTCTGTAGAAATCTGCGTATGCC10.6711409395973155No Hit
GGGTATGCCCTCCCCCATGCCATCCTGCGTCTGGACCTGGCTGGCCGGGACCTGACTGACTACCTCATGAAGATC10.6711409395973155No Hit
AACTATGCCTAAAGCAAATCAATAATCCCTTGAGATTTCTAGTGGCATTC10.6711409395973155No Hit
TCCTTGTGCAGTTCATGATGGTGTGGCAGCGGTATAGAGAGAATGGGTCCTGCAGCTTGGCCAGGCGCTCCT10.6711409395973155No Hit
ACTCGAAGTACTCTGAGGCTTGTAGGAGGGTAAAATAGAGACCCAGTAAA10.6711409395973155No Hit
GAAACCCCATCTCTACTAAAAATAAAAAAATTAGCAAGGATTGGTGCCAG10.6711409395973155No Hit
CCGGGGTGCGAAGTAGACATCCGTGTTCTAGCGCGATAACTGACTGTTTT10.6711409395973155No Hit
GTATATAAAAGAGTAGGGGTTTTACTTTAATGTCCTTTGAAGTATAAATA10.6711409395973155No Hit
GCAGTGGGGGGTCCAGCTGGAGGTGGAATAAATGCGGCAACCACAGAAAA10.6711409395973155No Hit
GTTGTGTGGTCGCGTCTCGGAAACCGGTAGCGCTTGCAGCATGGCTCACC10.6711409395973155No Hit
ACATTATTTTAGAGGAGCATGGCAAGCTGTGGGATACAGGCAGTTCTGCTCATCTTCCATACTCTACTACAG10.6711409395973155No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGATTTCTA10.6711409395973155No Hit
GAAGAGGGCCCTGGATGTAATTCAGGCAGGAAAACAATACGTCGAACACA10.6711409395973155No Hit
CCAGTAATACCTAATCGGCACGGTGAGGCAGGAGAATTGCTTCACCCCAG10.6711409395973155No Hit
CTAACAGACCTGATACTTTGGATCCAGCACTGATGAGGCCAGGGAGATTGGATAGAAAAATTGAATTTAGCTTGC10.6711409395973155No Hit
GTCCCAGATGATGTTTTAGCTTCTGACATTACTTCAGCCTCAATGTCTAT10.6711409395973155No Hit
AAGAAAACCACAGCTTTGTTCTGCATATCTTCCATCGTGCCAAACATGAT10.6711409395973155No Hit
GGCTAGGGTGACTTCATATGAGATTGTTTGGGCTACTGCTCGCAGTGCGC10.6711409395973155No Hit
GCTCTAGATATTTAACAAAATATTTGTCCAATCATTAACTGACCACTAAG10.6711409395973155No Hit
CAGTGAGAAAGGACCCATTAAGTGGGTTATCACTGATGCGGAGAAGAATG10.6711409395973155No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10.6711409395973155No Hit
GGCTTTCAGATTTCTCTTTTTAACCACATTTCTGAATGTATAAAAATACAAATTATTTTCCTACAGCCCTTTTTA10.6711409395973155No Hit
ATTCCAGGGGGCAGGCACAGAGCTGGAGGCTCTGCCCGGCACACCCACAG10.6711409395973155No Hit
CGGTATGGCACATTCCTTATTCCTTTGGCCCAGACAGCTTTGTTGAGCCTGGTGTCAATGCGCACATCTGGAGTT10.6711409395973155No Hit
TAGGAAAGTGTTGTCAACATTTTTTTCCTATTCTTTTCAAGTTAAATAAATTATGCTTCATATTAGTAAAAGAAG10.6711409395973155No Hit
AATTAATACCAATAGAAGGGCAATGCTTTTAGATTAAAATGAAGGTGACT10.6711409395973155No Hit
AGCTTGCAGAGTCCGTTGGTCGATTCAGCAGGGTCACCAGCCTCCGCCAACTGGACAAATCTGTCCACTCAAACC10.6711409395973155No Hit
GTCTTCCCCTCCATCGTGGGGCGCCCCAGGCACCAGGGCGTGATGGTGGG10.6711409395973155No Hit
CTGCATGAGGCCGAGGGCCACATTGTGACATGTGAGACGAACACCGGTGA10.6711409395973155No Hit
CTACTATTCAGCCCAGCAATCCCAGTGCTGAGTATATACACAAAGGAAAA10.6711409395973155No Hit
TGATGCATCTATGAGAGATGTGGTCTTTAACTACCTGCATGCCACAGCATTCCAGGGCACACCTCTAGCCCAGG10.6711409395973155No Hit
CTCAATGACTTGGGGTCCTCGACTTCGGAAGTTTATTGGGATAGGCTTCA10.6711409395973155No Hit
GCAGGGGTTCATGAAGAGGGTGTTGAACAGGGACCAGACGACATGGTCGG10.6711409395973155No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCT10.6711409395973155RNA PCR Primer, Index 27 (95% over 22bp)
GTTGTGACCTGAAGTTCACCATTAAAAGGGATTACCCAAGCAAAATCATGGAATGGTTATAAAAGTGATTGTTGG10.6711409395973155No Hit
GGGGATCATAGAGGAGATTATTGAAGATGGAGAAAGTTCTGATGTTAAAG10.6711409395973155No Hit
ACATTAGTTTTAATAAAAAAAAAAAAAAAAAAAAAAAAAAGGTGCCTGCCGTGTATTCTGTGTTAAAAAAACCAA10.6711409395973155No Hit
CATGGACACACGTTCTTGTCCAGGGAAGCCCCTACGGTTCCACACACCAA10.6711409395973155No Hit
TTTTAGAGCTGTGCGTGGGTGATTTAGAAACTTCAGCCCCACTGACATCA10.6711409395973155No Hit
AGTAGGGCCTGTTTCAGACTTCTGACCTAAAGATCTGTATGATAACACAT10.6711409395973155No Hit
CCAAAAGGCTGCTGACAAACCTGCCTTAATATATACCTGCACTGTCTGTAGGACACAAATCCCAGACCCTAAGTC10.6711409395973155No Hit
CTATATTATCATATTATCAGTCAATTACTACAGTTGGACTTTCAAACTAT10.6711409395973155No Hit
TGCCTCTTCTGAACAGGGACTGTTAGGAAGGTCACCACCAACCAGACCAG10.6711409395973155No Hit
GCTGTTATACTTGGTCACATATTCACATTGCTTGAAGGGGGCAGTCTTGT10.6711409395973155No Hit
TTCTTTTGCTGTGCAGAAGCTCTTTAGTTTAATTAGATCTCATTTGTCAA10.6711409395973155No Hit
TGGCCACAATGTGACCAAGCGTTCAGTTTCTACATTTCCGTTCGAAGCCA10.6711409395973155No Hit
GGGTGTATTTGTGTGTTAAGTAAATGTAGAGTTGGATTTGGCGTATTTTA10.6711409395973155No Hit
ATGAAAAAAGTATTCAAGATAATAAAATACAAATGGGAAAATTAACTAGATTACTTCTTCTAATAAATCCTAAG10.6711409395973155No Hit
GAATAGGGCACAGGTATATTGAGATCTTCAGAAGTAGCAGGAGTGAAATC10.6711409395973155No Hit
GAGATGCCAGAGGCTCTTGAAGTCACAAAGGGGAGGCGTGAAGAAATCCTGCATCTCAGTCCCTCACAAGACAG10.6711409395973155No Hit
TTTGAAGACAGGGTCTCACTCTTTTGCACAGGCTAGAGTGCAGTTGTGTGATCACAGCTCTTTGCAGCCTTGAA10.6711409395973155No Hit
CAATAATACTTGTGGACTTCAACACCCTACTTTCAGCAATGGACAGATCATATACATAGAAAATCAACAAAGAAA10.6711409395973155No Hit
CTACATACCTATTGAAATGAGTAAAATTAAGGACTGATACTATCAAGGATTGACATTAGAATGTGGAGCTGGAA10.6711409395973155No Hit
GAAGATACATTGAAAGCTTACTGTTAAAAACAAATGAGGATACGAATGAG10.6711409395973155No Hit
GGAGTACGAGGAGGTTAGTTGTGGCAATAAAAATGATTAAGGATACTAGT10.6711409395973155No Hit
ATTCATAAGATACCATATGGACAATGCCCGATTGCATGTCGTGGCAAAAACATGGAAGTGAGACTTATTTTTCT10.6711409395973155No Hit
AGATGGACCAGGCCTCCGGAACGGCACTGCCGCCCACACACTCAACACGC10.6711409395973155No Hit
AGATAAATGTAAACATTAAAAAAAAAGGAACCCTTTAGTATATTAACTTT10.6711409395973155No Hit
AGCTTGAAGAATATTCCTGTTTTGGAGACTGCCTATAAGTTAATATTGGGAGAAATGACTTGTGCCCTAAACAAC10.6711409395973155No Hit
ATCTAGTAGATAACTTTCTTACTTCAACAAAAACAAAGTTTATCAAGGATACACACAAATGGCTTTATCTGTGAA10.6711409395973155No Hit
ATCATGTACATGGCAGGCAATACTATCTCCATCTTCGCTACAATGATGTT10.6711409395973155No Hit
GTGCCAATGTAGGGTGCACATATTTCACAATATCCCCAAGTTCTTCAGAA10.6711409395973155No Hit
ACTCTATATAATTTTGTGCTGAGCGGATGGTATATTGATGGGAGGAAGTA10.6711409395973155No Hit
TCTTAACACCAACAGCAGCCTTCTTTCCTTTCTTACCTACCACAGGCTTCTTGCCTGCAACCGCCGCCTTCTCAT10.6711409395973155No Hit
GGTCAGCAGGGCAGATAATTACAGCAAAGTCTCTTAGGTCACTGGAGGTT10.6711409395973155No Hit
GTGCCTACATCCCCAGCTCCTTGGGCAGCTGAGGTGGGAGGACTGCTTGA10.6711409395973155No Hit
CTTCCGTTGTAGGTGATGAAATGGGTAAGGCTCCACACTTCAGATGTTGGCACTGCTATCAGCATGGTCTTTCC10.6711409395973155No Hit
CCCTCTGGGTCTCTTCTCAACTAAGTCCTCACTCATCCCCTGCGCCTCCA10.6711409395973155No Hit
CTGGTAGTGTCCTGGGCGTCCCGAGGCGAGGCAGCCACCGCCCAGGACGA10.6711409395973155No Hit
TATCCACGCAGAGTACGGGCCATTCCGTGTGGGGTGATGCAGCAGGTAAC10.6711409395973155No Hit
GAGTGAAACGAGCCGAAATCTCAAGAAGTGGCTTCGACGACGGACACCACGAGCAGCAGCCTGCGCTGGAAATGT10.6711409395973155No Hit
GCCTGGGACGAGCGTAATTAACGAGGGAGCATCGTTAGACACCGAGGACTGGAAGCTCCAAAGCCGCTTTCCGCG10.6711409395973155No Hit
GGTATCAACGCAGAGTACGGGGAAATTCAATTATGATGAAAAACCTATCT10.6711409395973155No Hit
CACACAAAAAGGCATCATACACATACCTTTCTTAAAACATGTCTAGAGAT10.6711409395973155No Hit
ACCAGAAGGCTCTGCACTGCAGGTCAGGGGATGATAAGGAAATGAACAGT10.6711409395973155No Hit
ATGGTGAAACGCATAAGAATCAATACAACTGCTGATGAGAAGGACCCTAC10.6711409395973155No Hit
CATCTTTCTATTCCTTGCATTGCACATGGTACTGGTAAGTTCTGTCATTT10.6711409395973155No Hit
AATCTCCGCAGACCGTTAGGGCGCACAACCGAAGATTTGCGAAGATTGGC10.6711409395973155No Hit
ACACTCAGGAGAAATCAAGCCGGCAAATTCTCTTTTTGCAGTTAAGATGT10.6711409395973155No Hit
GAAGTATTACGGTAACCGCGACGATAATAACCCGGACCAAATATAGCCTGATATGAGCGTGCCCGTCCATAGTCC10.6711409395973155No Hit
GTTTATAATATGTTGCATTTGTACTTAAGTCATTCCATCTTTCACTCAGG10.6711409395973155No Hit
ACATCATAGTTGGTGTTACGGAGGTTTTTGAGCATCTTTTTCCTCTGGTC10.6711409395973155No Hit
GCCATAAGGAGCAACAGAAAATGGTCTATGCTTCCCTCTATGCCTGAACT10.6711409395973155No Hit
ACATATCAATTCCTGAATTTCCAGGCATCATAGACCCAGAAGCTCACGCAGTGGTTTCCAACATTTGTTAGCATG10.6711409395973155No Hit
GATGAGCCATTAACAATGGTGGAGATTCTTAATCACTGAAGCACTTGATA10.6711409395973155No Hit
TAATAAACAAAGAACAAAAAATATGAACTAGTATTGACAGAAAAAAATTA10.6711409395973155No Hit
GAGTACGGGGGCGCAATAGATATACTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATACCAAGGA10.6711409395973155No Hit
GATAAGATAAGGGACCTGTTAGATGTTTCAAAGACCAAACTTTCAGTTCA10.6711409395973155No Hit
GGTTAAAAGATTCCATTTAGTTCCCTTGAAAGAGACCCATGCTTCATAAAAGTGGCTAGAGCCACCTGTGCTCTC10.6711409395973155No Hit
ATCTCCTCCTCCCACGCGGGGCACGGTGAGAAGCCTTCTGGAGGCTGCACGTACCTTCACGGCCAAGACTCCTCA10.6711409395973155No Hit
GCACTACGCACAAGACGCCTCAACCGCAGTTTCATCTATCGCCCTCATCACTCTAGACGTAAATTATTCCTGCAT10.6711409395973155No Hit
CTTGACATGCTTTGAATTCAAGTCCTGTTAGACATGGGAACAGCTTTTCC10.6711409395973155No Hit
TCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGCCAACCAGC10.6711409395973155No Hit
GAGCAGGGCCTGACTGCGCAGGTCCTGGATGCCTCATCCGTTACTTTGAA10.6711409395973155No Hit
CAACTTCAGTGTCGGATGGATGAAACCCAGACACATAGCAATTCAGGAAA10.6711409395973155No Hit
GTTGAGTGAGGGGGGGGCGTAGTGGAGACCCACGGCAGGCCTGAAGAAGAGCGGCGGCCGAGCCCGCCTTCCCTG10.6711409395973155No Hit
ATTATACCCTAGCCAACCCCTTAAACACCCCTCCCCACATCAAGCCCGAA10.6711409395973155No Hit
GCTGGAGGGGTCAGAGCTTTGTGGAGACAGAAGTAGGAGAAGTCACGCTG10.6711409395973155No Hit
GTGTTATTGTCATTAAGGAGAGAAGGAAGAGAAGTAAGCCGAGGGCGTCT10.6711409395973155No Hit
CTCTGAAGGATGCAGTTGAGGTTGATCCAGGTTTATCCGAATGTGCTACCTTTCTGAGCCTTAAACCTTCATCT10.6711409395973155No Hit
CTTTAATAGTGAATAATTGCAGTCCAAGAACACAAGAAAACATTCCGCAA10.6711409395973155No Hit
GTTCCTGAGCTGAGAGGACAGTTCCCTCTCAGCATAACCCTGGTCCTGCG10.6711409395973155No Hit
ATCTTACATTGTGGCTCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAG10.6711409395973155No Hit
GTATCAACGCAGAGTACGGGCACTTTTCTCTGACTTGCATTGAGCTTTTGTTATCCTCTTTTAATATTTCATT10.6711409395973155No Hit
ATACTTAACTGTATTTAGATTACATAAAATCAAAGATACAAAAGTAGATT10.6711409395973155No Hit
CAATAATCAATATAAAACCCCCTGCCATAACCCACTACCAAACGCCCCTCTTCGTCTGATCCGTCCGAATGACAG10.6711409395973155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTGGGATTGTT10.6711409395973155No Hit
TACAAGTACATCCAGGAGCTATGGAGAAAGAAGCAGTCTGATGTCATGCGCTTTCTTCTGAGGGTCCGCTGCTGG10.6711409395973155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAACGTGT10.6711409395973155No Hit
TCCAATTTCTGACAAAATGTTTATATTTCACATTTTGCATCAGTGGCAGT10.6711409395973155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGCGTTTTTATTTTATTATGTTTTTAAATTC10.6711409395973155No Hit
ACTCAGGTGAGTGTTAGCTTTATTGGGGAGGGGGTGATCTAAAACACTCT10.6711409395973155No Hit
ACCATAGCCACTGCCCCCTCCCCTGGATGCCCAGTAATGTATGTCTACTG10.6711409395973155No Hit
CGCTGGGACCCTGGTCTTGCTGTCCCCCGCTGGCCTCCTGCCCAAGCGAC10.6711409395973155No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)