FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003683378

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003683378
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654516
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92860.3498189500458841No Hit
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT84230.3173083153388414No Hit
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC82540.31094180634059093No Hit
GAGTAAGCCATTCCCTTTAGACTTGGTTGGGCAGCTGTCACATGGCTGAC70930.2672050196721361No Hit
GAATATAAATATCGAAAACTTGTTCAGAGAATTGGACATCAATAGTGACA63590.2395540279282551No Hit
GTGTATGATGCTGATGGCAAAGTTGATCTTTGCCTGTCATGAGAAGCTGCATGAGAACAACCCACGTGGGCATGG49780.18752947806681144No Hit
CTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCTGCTACTCCTTGTGGCTGTCTTTGTGAGATGCCA47060.17728278902820704No Hit
TTTATATTCTGCACAAACTGAGGACACTCAGTAGTGACCATTTTCTTGAA45060.1697484588527626No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43600.1642483978246882No Hit
TCCCAGAACAAAGGCCATTGAGTAAGCCATTCCCTTTAGACTTGGTTGGG42830.1613476807071421No Hit
ATACAAGGAAATCACCATGCCCTTTACAAGAATGACTTCAAGAAAATGGTCACTACTGAGTGTCCTCAGTTTGTG42320.15942642651240377No Hit
TCCAGGGACCCAGCCCTAGGCCAGAAGCTCTGCTACTCCTTGTGGCTGTC41540.15648803774398046No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41510.1563750227913488No Hit
GACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCTGCTACTCCTTGTG39810.14997084214222103No Hit
GTGTATATTGAGATCTCCCTTTAAAGTAGATGTCAGAGGGAAATGTCTCT39760.1497824838878349No Hit
GTATGATGCTGATGGCAAAGTTGATCTTTGCCTGTCATGAGAAGCTGCAT38510.14507352752818214No Hit
ATCATACACTCCTCAAAGCTCAGCTGATTGTCCTGGTTTGTGTCCAGGTC37260.1403645711685294No Hit
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC37120.1398371680562483No Hit
GATAAAGACTTATTTATTATTTATCTTATCATTTCCCAGAACAAAGGCCA36580.1378028989088783No Hit
CAATTATTCCAATATACAAGGAAATCACCATGCCCTTTACAAGAATGACT36460.13735083909835163No Hit
GCCCTAGGCCAGAAGCTCTGCTACTCCTTGTGGCTGTCTTTGTGAGATGC36160.13622068957203498No Hit
CTTTTATCACCATCGCAAGGAACTCCTCGAAGTTAATTGCATTGTCACTA35350.13316928585097998No Hit
CAATATACACAGTGCAAGTTGCAAGGGCCCTTATTGAGAGGAGCTTTACA35240.13275489769133056No Hit
TTACAAGAATGACTTCAAGAAAATGGTCACTACTGAGTGTCCTCAGTTTG32400.1220561488421995No Hit
GTCTTTGTGAGATGCCACACCCACTTTTATCACCATCGCAAGGAACTCCT31970.12043626785447895No Hit
CTGCTACTCCTTGTGGCTGTCTTTGTGAGATGCCACACCCACTTTTATCA31920.12024790960009281No Hit
ATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCTGCTACTCCTTGTGGCTGTCTTTGTGAGATG30080.11331632583868397No Hit
CCCTGACACCCTGAGCAAGAAGGAATTCAGACAAATGGTGGAAGCACAGT29890.11260056447201675No Hit
ACCTCATTGATGTCTACCACAATTATTCCAATATACAAGGAAATCACCAT29250.11018957881587454No Hit
CTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCCCA28450.10717584674569676No Hit
CTTAATTACTTCCCACAGCCTTTGCCATGACTGTGGCCATGCCCACGTGGGTTGTTCTCATGCAGCTTCTCATGA28060.1057066523614851No Hit
ATCCAGGGACCCAGCCCTAGGCCAGAAGCTCTGCTACTCCTTGTGGCTGT27710.10438814458078234No Hit
GTATCAACGCAGAGTACGGGGATCCTTTGTCAGCTCCGTCTTCAAGACAT27640.10412444302464179No Hit
GAACAAAGGCCATTGAGTAAGCCATTCCCTTTAGACTTGGTTGGGCAGCTGTCACATGGCTGACCTCTTAATTA27480.10352169661060621No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)