FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003683379

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003683379
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2654516
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80850.30457529734234035No Hit
ACTTTATTCTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCT73330.2762462158826694No Hit
CTACTGAGTGTCCTCAGTTTGTGCAGAATATAAATATCGAAAACTTGTTC71370.26886257231073385No Hit
GAGTAAGCCATTCCCTTTAGACTTGGTTGGGCAGCTGTCACATGGCTGAC62750.23638960925456845No Hit
GAATATAAATATCGAAAACTTGTTCAGAGAATTGGACATCAATAGTGACA57720.21744076886332572No Hit
GTGTATGATGCTGATGGCAAAGTTGATCTTTGCCTGTCATGAGAAGCTGCATGAGAACAACCCACGTGGGCATGG47220.1778855354422426No Hit
CTGTAGACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCTGCTACTCCTTGTGGCTGTCTTTGTGAGATGCCA43100.16236481528082708No Hit
TTTATATTCTGCACAAACTGAGGACACTCAGTAGTGACCATTTTCTTGAA40650.1531352608159077No Hit
GTGTATATTGAGATCTCCCTTTAAAGTAGATGTCAGAGGGAAATGTCTCT39320.14812493124923715No Hit
GTATGATGCTGATGGCAAAGTTGATCTTTGCCTGTCATGAGAAGCTGCAT37070.13964880980186217No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36950.13919674999133552No Hit
CTCTTATAGGCCTCACAAGGTGATCTGACCACACCCACCTGCCACATCCC36900.1390083917369494No Hit
ATACAAGGAAATCACCATGCCCTTTACAAGAATGACTTCAAGAAAATGGTCACTACTGAGTGTCCTCAGTTTGTG35640.13426176372641943No Hit
GACATATCCAGGGACCCAGCCCTAGGCCAGAAGCTCTGCTACTCCTTGTG35640.13426176372641943No Hit
TCCAGGGACCCAGCCCTAGGCCAGAAGCTCTGCTACTCCTTGTGGCTGTC35580.13403573382115608No Hit
GCCCTAGGCCAGAAGCTCTGCTACTCCTTGTGGCTGTCTTTGTGAGATGC35070.13211447962641776No Hit
TCCCAGAACAAAGGCCATTGAGTAAGCCATTCCCTTTAGACTTGGTTGGG34980.13177543476852277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33710.12699113510711557No Hit
CTTTTATCACCATCGCAAGGAACTCCTCGAAGTTAATTGCATTGTCACTA33600.12657674694746612No Hit
ATCATACACTCCTCAAAGCTCAGCTGATTGTCCTGGTTTGTGTCCAGGTC32880.12386438808430615No Hit
GTCTTTGTGAGATGCCACACCCACTTTTATCACCATCGCAAGGAACTCCT31930.12028558125097004No Hit
CAATTATTCCAATATACAAGGAAATCACCATGCCCTTTACAAGAATGACT31650.11923077502640782No Hit
CTGCTACTCCTTGTGGCTGTCTTTGTGAGATGCCACACCCACTTTTATCA31090.11712116257728339No Hit
GATAAAGACTTATTTATTATTTATCTTATCATTTCCCAGAACAAAGGCCA30900.11640540121061617No Hit
CAATATACACAGTGCAAGTTGCAAGGGCCCTTATTGAGAGGAGCTTTACA27940.10525459255095844No Hit
GTATCAACGCAGAGTACGGGGATCCTTTGTCAGCTCCGTCTTCAAGACAT27700.10435047292990511No Hit
TTACAAGAATGACTTCAAGAAAATGGTCACTACTGAGTGTCCTCAGTTTG27150.1022785321316579No Hit
CCCTGACACCCTGAGCAAGAAGGAATTCAGACAAATGGTGGAAGCACAGT26580.10013124803165624No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)