FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003683636

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003683636
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69399
Sequences flagged as poor quality0
Sequence length65-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8531.2291243389674202No Hit
GTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1570.2262280436317526No Hit
ATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1350.19452729866424587No Hit
GAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAAATCTCGTATGCCGT1330.1916454127581089TruSeq Adapter, Index 13 (96% over 33bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1310.18876352685197195No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1030.14841712416605427No Hit
GGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT940.13544863758843786No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT880.12680297987002695No Hit
AGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT820.11815732215161602No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)