FastQCFastQC Report
Sun 11 Sep 2022
EGAF00003683851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003683851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3192701
Sequences flagged as poor quality0
Sequence length35-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT132970.4164812176273318No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT70910.22210034701025871RNA PCR Primer, Index 39 (95% over 23bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN66030.2068154831911914No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52310.1638424644211907No Hit
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT43120.13505805899143075RNA PCR Primer, Index 39 (96% over 25bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35380.11081526268823796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)