FastQCFastQC Report
Wed 31 Aug 2022
EGAF00003684133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003684133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length53-76
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGC27.142857142857142No Hit
TTATTTACTGGGAGTTAATACTGAGAGAGTATATGCAAAGGCTGAGGGCACCATGCCATCTTCACAGAACAAGAC13.571428571428571No Hit
ACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTCAGTTTTATGGATAATTGGGATATT13.571428571428571No Hit
GAGTACCTGTCATGTGCCAGGGCACAGTGCCAGGCTCTGAAGTTATAATA13.571428571428571No Hit
CTTCTGGGTCTGCTGACAAAGCATGATCAGGACCTTCCATATTTTTATCTAAGGTAAAGTGCTTCTCAATAACAT13.571428571428571No Hit
GTGTTGTCCAGTTCACTAGTCTTCTCTTCTGCAGTGTCTAAGCTGCCATT13.571428571428571No Hit
ATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACGCTTGAGGAAGTAAAAGAAGCGGATTTAATT13.571428571428571No Hit
CTTAGATATTTTAAAGAGGCATCTATCACATAAGGCATCATTATAACTAA13.571428571428571No Hit
TCGTATATCCGACTGTAGGTGCGTAACTCGCGACTAGGTGGCTCTAATCTGCGTTGGTTGTCGCTCACAGTGT13.571428571428571No Hit
CTATAATTATGCTCACTGAACAAATACCTTGAAAGGGAAGTAATTCCAAG13.571428571428571No Hit
TCTTTATGGTAAATGGGCCAAACGTTCAGTTTTGTGAGAGCTTGCATTGT13.571428571428571No Hit
GGTGACTAGGATGCTCATTTGAATGTCCCCAATTCGCGTCATCGATCCGGGTAGCCTCGTAGGGGATAGGGGAGG13.571428571428571No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG13.571428571428571No Hit
GTATATGATTTTGCTCTGCAAGAACCTTTAGGCTAGGCCTTATTAACCTCTCTCTCCTACATTTGCTATGCGTCT13.571428571428571No Hit
GATTTATTCAGGATCTTCCGACGACATTGATTGCTGCATTCCGCTCAACG13.571428571428571No Hit
TAGTTGGGATTTTAATATCATTAATAGCATGATAGTGATTGTTTTGAAAA13.571428571428571No Hit
AGATAAGGATATTATCTCATATGGCGGAGTAGAACGTCTTTAGCGGCCTG13.571428571428571No Hit
ACCCACAGGTCCTAAACTACCAAACCTGCATTACAAATTTCGGTTGGGGCGACCTCGGAGCAGAACCCAACCTCC13.571428571428571No Hit
CTTTTGTATCTTCATTGTGGGCCATAGCCTTTATCATCACTAGATCTTTG13.571428571428571No Hit
CGGCATAAGGGAGAAATGGAGAATGCTTTAAGGTCATATTCAAATATTAC13.571428571428571No Hit
ATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCA13.571428571428571No Hit
GTCTTAGCAGTAGTATATAAGGAATTTATCCCATTTTTAGTTATAATGAT13.571428571428571No Hit
ATTTTCAAATGTGTAGCATTAAGAAAAAGGGCAAGTGAAGCTCAACTGAA13.571428571428571No Hit
ATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTG13.571428571428571No Hit
GTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCTATCATTGTTGGACATTTGGGTTGGTTCCAAGT13.571428571428571No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGGAAGGAGACATAACGATAGCGACT13.571428571428571No Hit
GCCTAGTAAAACCACGATCAGCCGACGGTCTGGATGCCGACGCCCGTATACTG13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)