FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003684134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003684134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28
Sequences flagged as poor quality0
Sequence length53-76
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGC13.571428571428571No Hit
CCTCAAGCAGCCGAAGCACTGTTTTTTCATGTCCCGCATAATCCTCATTT13.571428571428571No Hit
CTGCAGACCTGAGTGTCATAGAGCCAGACCCCTGACTTGCTGCCAGTAAA13.571428571428571No Hit
TTTTTCTTGGTACCTATTCCTCCAGGAATTACTGACCATAGTGCTCGTAC13.571428571428571No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA13.571428571428571No Hit
CGTTTATTGTAAGCCGTCAGCGTCGGGATATCATCTGCTTCAAGCTCCTC13.571428571428571No Hit
GGTTCACGCCATTCTCCTGCCTCAGCCTTTTGAGTAGCTGGGACTACAGG13.571428571428571No Hit
GTGCTGGAGAGGATGTGGAGAAATAGGAACACTTTTACACTGTTGGTGGG13.571428571428571No Hit
ATGCCAAGATTACGGAACCCGATCCGGGTATCGAACACCAGACACTGTGA13.571428571428571No Hit
AAACTGTAACTAAGTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGC13.571428571428571No Hit
CTTCTAGATCCATTTTCCTACCTAGACCTCTCTTCTAAACTCCAGTTATG13.571428571428571No Hit
GGTGTATAATGTCTCGGACCCGTCGCAACGGTTGTTAATATGACAGACCG13.571428571428571No Hit
ACTTAGTTTAGACGTAGCACCTTAGCAGACTTTCCTCGGTCCTTAACATG13.571428571428571No Hit
GAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACTATCATGCTATTAATGATATTAAAATC13.571428571428571No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG13.571428571428571No Hit
GGTCAAACAGTATTTCCCAAGGGGACTTAAGCGCGGTGGCCTCCCCTATC13.571428571428571No Hit
CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG13.571428571428571No Hit
TCTTTCTGCTCCGCTGAGAAGTACACCTTTTTGCTAGCATCTCGCACGGC13.571428571428571No Hit
TTCTTCAACAAGGTCTTCAAGCTGTCTCTGTAGAGCACACTTTTCTTCTA13.571428571428571No Hit
CTATAAACTCATAGCAGGTTGTGCAAACATTGCTCAAAAGTGTGTACACAGCCCTAAGACGTCTTGCTATACTTA13.571428571428571No Hit
ATTCTATGCAGATATACACACGTTGATATGCATTCCAAATCTTAAAATATCTGTTCTTTTAAATTCATATTAGAT13.571428571428571No Hit
GTTAATTAGATACATGGAAGGTAGGTGTGTGTGTGTGGGCAGTGGGGGTG13.571428571428571No Hit
GGATTGCGCTGTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTG13.571428571428571No Hit
CAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCCAACTATA13.571428571428571No Hit
GTAGTTTCTGTTGCCTTAGGAGCGGATGTTATTGAGAAGCACTTTACCTTAGATAAAAATATGGAAGGTCCTGAT13.571428571428571No Hit
TTTCAATACTGAGCACAGTGCTGGCACAGAATATGAACTCACTATATCTT13.571428571428571No Hit
CAATTGGTAGAGTTCCAACATCTCCTGGAAAAACCTTTGCTGGATATTTGTTAAATATCAAT13.571428571428571No Hit
ACCCTCGTAATAGCGCTGCGTGCGTCCTAAATACACTCGCTGTTGAGATA13.571428571428571No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)