FastQCFastQC Report
Sat 3 Sep 2022
EGAF00003684340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003684340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930433
Sequences flagged as poor quality0
Sequence length35-76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137351.4761944170079953No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82260.8841044975833832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71400.7673846477930168No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA69950.7518005057860158No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64210.6901087988065772No Hit
GCGTTGATACCACTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA52180.5608141585691823No Hit
ACTCTGCGTTGATACCACTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA23550.25310796156198245Clontech Universal Primer Mix Long (95% over 22bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21430.23032287117933262No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20840.2239817375351046No Hit
GTTGATACCACTGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19390.20839759552810358No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18950.20366861450528947No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTAAAAAAAAAAAAAAAAAAAAA16980.1824956767440536Clontech SMART CDS Primer II A (96% over 25bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA14790.15895824847141063No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14130.15186477693718947Clontech SMART CDS Primer II A (100% over 21bp)
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13890.1492853327429272No Hit
GTATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13410.14412644435440272No Hit
AAAGTACTCTGCGTTGATACCACTGCTAAAAAAAAAAAAAAAAAAAAAAA13310.1430516759401268Clontech SMART CDS Primer II A (96% over 25bp)
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGAGATGTTGGAACTCTACCAATTGGAG11740.12617781183599464No Hit
ACGCAGAGTACTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11240.12080396976461498No Hit
TATCAACGCAGAGTACGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11000.11822452557035273No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACCGCCCTTAGACGTCTTGGTATACGGA11000.11822452557035273No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG10580.1137104982303938No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA10450.11231329929183509No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10020.10769179511044857No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA10000.1074768414275934No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9320.10016841621051703No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)