FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003685567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003685567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247
Sequences flagged as poor quality0
Sequence length35-76
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN421.8691588785046727No Hit
CTCATGAGAACTCACTCACTATCACAAGAGCAGCTGCCCCCATGATTAAA50.22251891410769914No Hit
CCAAACTTTGGAGCAGTCACACATCTGTCGTAAAACGAAAGCAGGTGTTC50.22251891410769914No Hit
CCTGGCAAAGCCATGGCAGATGGAGGAGCAAGACTGACAGGCACGCAGAT40.1780151312861593No Hit
CGCGGGAGAAGAAAGACTGGGCGAGCTGGCCCAGCAGACAGCATGTGGAG40.1780151312861593No Hit
CCTGGGTGGATTTTCTACCCGAGCACTGGACGTAGGAGGTAGAAGGAAAG40.1780151312861593No Hit
GCTGGGACTACAGGCGTGAGCCACTGCGCCTGGCTGACATATTTTACAAT40.1780151312861593No Hit
TGGGTGTGTTTGGAGATGATTTTACGGGCTCTTTCTGGAGAACAAAAGTAAAACGTTACAGTGTTCCGATGAGAC40.1780151312861593No Hit
AGCTCAAGGATTGTAAATGCACCAACCAGCACTCTGTGTCTAGCTAAAGG40.1780151312861593No Hit
GGTGTGTTTGGGGGTTGGGTTGACTTCTCTTTCTCGGGTGTTTTTCAGAA30.13351134846461948No Hit
GGCTTCAGAGGTGACCCAAAATGGGATGAGCCTTTCAGGAGATATTTTTC30.13351134846461948No Hit
ATATTTTCTGACCCACTAGACAGGCTCCCCATAATACTAGCATACAAGGT30.13351134846461948No Hit
CTTCTTCACAAGGCAGCAGGAAAGAGAGGGGCAGGGGAAATGCCCCTTAT30.13351134846461948No Hit
GTGTTGGGTGTGTTTGGTTGTTGTGGGTAAGAATGAATAGTGAGGGCCAG30.13351134846461948No Hit
CAGGGAAACTAAGCATCATATATGAAGAAAAGATACAGTTGTTTTCAGACAAACAAATGCTGACAGAATTCACCA30.13351134846461948No Hit
GGTGTGTTTGGTGGGGGTGTGACGTATACAAATCAATAAATGTGATCAAT30.13351134846461948No Hit
CTACTCGGGAGGCTGAGGCAAGAGAACTCTTTGAACCCAGGAGGTGGAGG30.13351134846461948No Hit
TTTGGGGTTTTGTTTGGTTCCATATTTTTGCAAACTGCAAATTGTGCTGC30.13351134846461948No Hit
GGTGTGTTTGGGGGTGGGGGTGATAGTGTGATGTGCATGAGTGTCTTGAG30.13351134846461948No Hit
GTCTTCTCAGGATGTCCTGTCTGTTGTTTCTCCCTCTGCCACATGGTTTTAGATGCAATCCAGGATTTCCAGGA30.13351134846461948No Hit
TGGGTGGGGAGATATTTCCTTTTCCAACATAGGCCTGAAAGCGCTCCAAA30.13351134846461948No Hit
GTTGGGTGTGTTTGGTGGGTTGGTGCCAGGTAAACAGTGGGTGCTCAACA30.13351134846461948No Hit
CAGAGGGAGGACAGCTGGAAGAAGGTGACCTCTGAGCAAGGGGCTCGAGA30.13351134846461948No Hit
GGTGTGTTTGGGTTTGTGGTTGCTTCAGGATGGAGACTCGTGTCCAGATCATGAGGGGTGGCCCCAGGGATGACC30.13351134846461948No Hit
TTTGGTGTGTTGGGTGTGTTTGGTTGGGTGGTGCATGCCTGTAATCCCAGCTACTCGGGAGGCTGAGGCAGGAGA30.13351134846461948No Hit
TATTACATCATTCCTATCCCTCTACCTGTTTTTCAGTTTAGTTCAATCGCATACGTATTGCTTAATTGCCTGATG30.13351134846461948No Hit
TGTTGGGTGTGTTTGGGGGTTTTGGGCAGGTCTGGAGATGGCAAGGGAGGGATCCTGCTTGGATGAAAAGTCTTC30.13351134846461948No Hit
CTCAGATATCCTAGGCAATGGGTGAGCCCATGGAACTCTCAAAAATTCCT30.13351134846461948No Hit
TGACAAAAGCAAATGTCTTGCAAATATATGTAGAATGATACTTCAGAGAA30.13351134846461948No Hit
GCTACTCAGGAGGTTGTTCATTCACAAATAAAGAAGAAATGCTATCAAGGATGGCTGGATCATCCTTGTTCATTC30.13351134846461948No Hit
TTCGCTCTTGTCACCCAGGCTGGAGTGCAGTGGTGCCGTCTTGGCTCACT30.13351134846461948No Hit
TGGGTGTGTTTGGTTGTTGGTGGTAAAAAGGAAGGAGGAAGGACCCTATG30.13351134846461948No Hit
TTTGGTGGTTGTGGTGGGATGGCGCCTGGTGAGGTTGCATTGCTGAGTTT30.13351134846461948No Hit
TGTTGGGTGTGTTTGGGTTGTGTTTTGAGGGGAGGTGGGCGTGTTTCTCC30.13351134846461948No Hit
AGCATGCGACTGCTCTCTGCCTCAATTTCCTCTCCTGTGAAATGGAGATG30.13351134846461948No Hit
CTCCAGCCTGGGTGACAGAGTAAGACCCCATCTCTTAAAAAGTAATAAAA30.13351134846461948No Hit
ACCATGAATGTATCAAGCCATACTCTACCTCTGCAGAAATAAATTGAGGC30.13351134846461948No Hit
TAGAAATGCTACTGATTTTTTACATTGATTTTGTATCCTGAGACTTAGCC30.13351134846461948No Hit
CCCCTTCTTGCTGGCACTGTCAATGGATAGATAAGAGGGCTGGAGAACAA30.13351134846461948No Hit
GTTTGGTGGGGTTTTGAGATTTTTCTAAAGACTCTTGGTAGATGTTTAGT30.13351134846461948No Hit
GGGTGTGTTTGGGGGTGGGGTTGGATAACTGCACCTCTCAGGCACCTTTT30.13351134846461948No Hit
TTTTTAAGATGGTGTCTCACTATGTTGCCCAGGCTGGAGTGCTGTGGCAC30.13351134846461948No Hit
CCTATTAATAAAGCCCAGTGGTACAGGCATCCTGGTACTAGTGGAGGGAA30.13351134846461948No Hit
CAATGAGCCGAGATTGCGCCCCTGCACTCCAGCCTGGGTGACAGAGTGAGACTCCATCTCAAAACAAGCAAACAA30.13351134846461948No Hit
CACCCAGGCTGGAGTAGAGTGGCACGATCTCGGCTCACTGCAACCTCTGC30.13351134846461948No Hit
TTGTGGGGTTAAAAAGCCGGGAGCAGGGCAGGTATGGGAGACAAATGTTT30.13351134846461948No Hit
AGCAGTTTGGGAGGCCGAGGCGGGCGGATCACCTGAGGTAGGAAGTTCGA30.13351134846461948No Hit
CCATTTCATACAATAAGAGCTAACTAACATGATCTCAACTAAAACTGCAG30.13351134846461948No Hit
TGTAGCTAAACCTTCCACCAGCGCTTGAGAACTTAATTTGAACCGGATCC30.13351134846461948No Hit
TGGGTGTGTTTGGGTTTTTGGTTATTTTACGTTGACATAGAGTTGAGAGAAAAAACATTACATTCTTTTTAAATG30.13351134846461948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)