FastQCFastQC Report
Thu 1 Sep 2022
EGAF00003685697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00003685697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1541
Sequences flagged as poor quality0
Sequence length69-76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGGGGGGGGGGGGGGGGG191.2329656067488644No Hit
GAAGAGCACACGTCTGAACTCCAGTCACGCTCATGAATCTCGTATGCCGT30.1946787800129786TruSeq Adapter, Index 9 (96% over 33bp)
AGTCGGGGGGGGAGCTTAGGACATTAGACAGAAATGAAGATAAGGTCTGACTCTTAGCATAGCTGGTGGGCATGG20.12978585334198572No Hit
ATGAGGGGGGTGGGCAGGAAGTAGTAAGTTCGAGAGTACCACAAAGTTCT20.12978585334198572No Hit
AGGATGGGGGGTAGCCAATCCCGAGTTCTGTCTGGGCATGTATCACAGCC20.12978585334198572No Hit
GGGGTGTGGGTGGCTACAGGAGGGATAGCATTAGGATAAATACCTAATGT20.12978585334198572No Hit
ATGCGTAGGGTTGGAATTATCACACTGATTTTCCTCTCACATTTGTTCAT20.12978585334198572No Hit
GTGAGTCGTGGTTGGAGAAATAACATTAAGATAAAATATTCTTCCAATTT20.12978585334198572No Hit
GGGTGGGTGAGCTCGGTCCCAGACCCAGGGTACACTGGGCACTGCAGACCCCTCCCGGGGAGGGGGGCCTGGGT20.12978585334198572No Hit
CGTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGCTCATGAATCTC20.12978585334198572TruSeq Adapter, Index 9 (97% over 38bp)
AGGGTGGGGGTGTGGCTGTCAAAGATAATGAGCTATGGAATCACAAGATTCCTTCTGGGAAAGTAGAAGAGAG20.12978585334198572No Hit
AGGGGGTGTGGGAGGTGTAGGCAATTTAGGAGACAGCAAATGAATTCTGA20.12978585334198572No Hit
ATTGTGCTGGGTGTTACCTTTAAGATAGATTTAACTCTTTTGCTTTGCTT20.12978585334198572No Hit
ATTGTGGGGAGGAACGGTGCACTCTGGCACAGATACTGCGCTTTCCTCAC20.12978585334198572No Hit
GGGAGGGGGAGTACAGAAAATATGATTATAGTTCATTTTCATGCCTGCCA20.12978585334198572No Hit
AGTGTTGTGGGGAGAGGTCCCTACTGAATGGCGAAGGAGCTGTCTGCAGA20.12978585334198572No Hit
AGTGTGTGGTGAAGATGCCAGCTGTTATTGTATTGGGATCTCTCTCTTTA20.12978585334198572No Hit
GTGGGGTGGGTCTAAATATTAGGAGTATATGTGAAAAAAACATGATAAAGGTTCCCTAGCTAAAGAAAAGGAT20.12978585334198572No Hit
GGTATGAGGGTCCTGTCTGTTAGAAGGAAAACTAACAAACAGAAAGGACA20.12978585334198572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)